LOCUS       sequence-247607-       16201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16201)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX185
FEATURES             Location/Qualifiers
     source          1..16201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6315..6339)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6368..6457
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6491..6743
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7172..7424)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7892..8917)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7892..8917)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8927..9271)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9413..9430)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9413..9429)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9422..9444)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9569..9588)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9779..9800
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10047..10071
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11667..12815)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13114..13908)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14100..14867
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15560..16191
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgaatca
     4621 ctctgttctt aattttgaaa tggatgacga caacccaaat attcctataa cttctggtat
     4681 caccaccccg tcaaagaagt ccacagtaat ggatgaagat ataatggagc taatatggca
     4741 aaatggacaa ataattggcc aaagccaaaa tcaaagatct caaacggtag cagcttcagc
     4801 tccaccaata gttttacaag aggatgaaat ggccacgtgg cttcagtatc cacttgacga
     4861 ttcttctttc gagcgtgatc tctatgccga ttttctctat actacgccga gctcctccgt
     4921 tgtcaccgct gccgttccgc cgccaggaga gattgatact gccacgacgg agatccgtcc
     4981 gaatcagcct ccgcctcaat tgagatgtac tgagggagaa ttttcacatc gcttacagaa
     5041 tttcgggcat ttttcacgat tgcctaaagc aaaatcgcaa aattatacat caacttctcg
     5101 taacacagct agggtatcaa cgattgtcga ttcgaatgaa actccaatgg cagagttttc
     5161 acgcgtatcg gataaagtag cgccagtttc cgccgggaat gacgaaggaa aggccacgac
     5221 tgatactact actacgacgg cggtggaaac aaccagcggc agggaaacgt cgacgacggc
     5281 atgtgagttc acggggacgt catcggcaag tggttcgaga ggtagtgtaa gtgccagcgc
     5341 ggaaccaccg ccgccgcagc ctccgcagga ggcggcgctt atgccggcgg agaatcggaa
     5401 acggaaagta agagaaaggg gaaaggagga caatgcagaa catagtgagg atgctgaatt
     5461 tgagtcagct gatgcaaaga agcaaaccag cagttctaca tccacaagga gatctcgtgc
     5521 cgcagaggtc cataatcttt ctgaaaggag acgtcgagat agaataaatg agaagatgaa
     5581 ggctctacaa gaactcatac cgcggagcaa taagtcagac aaagcttcaa tgctggatga
     5641 agcgattgag taccttaaat cattgcaaat gcaagtgcag atgatgtcca tgggatgtag
     5701 catggtccct atgatgtatc ctggagtccc gcaatacatg ccaacgatgg gaatgggtat
     5761 gcgtatgggg atgggtacgg gaatgggtat gaaccggcca atggttccat atccatctct
     5821 aatgccaggt ccggcaatgc agaatgcagc tgcagtagca caaatgacac ctagatttcc
     5881 tgttccagca tatcatttgc cgtcagttcc tgtacctgat ccgtccagaa tccgagcagc
     5941 gaaccagcca gattatccaa taatgaactc acttgttgga cataatagta atcagcccag
     6001 acttccaaat cttagtgatc catatcaaca atattatgct ctacaccagg cacaagtgtt
     6061 accacagaat cagcgagtgg aacagcccag cagcagtaac ccaagcagcc gcatagaaga
     6121 aggaagtcca gcaaatcatc aagctgtttt tcgggtggtc catggaggag gagatggagg
     6181 tggtagagat ggggttagag aaatgaggtt gattacaagg tggatcaacc aagagagctt
     6241 ttcaccaacc aaattttgta gtgtaagaga attaagagaa acaagagtgc agtctagact
     6301 ggctgaaccc ggggacccag ctttcttgta caaagtggtt gataacagcg ggttaattaa
     6361 catcttttac ccatacgatg ttcctgacta tgcgggctat ccctatgacg tcccggacta
     6421 tgcaggatcc tatccatatg acgttccaga ttacgctgct cagtgcagcg cttagagctc
     6481 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     6541 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
     6601 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     6661 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     6721 ggtgtcatct atgttactag atcgggaatt agcttcatca acgcaagaca tgcgcacgac
     6781 cgtctgacag gagaggaatt tccgacgagc acagaaagga cttgctcttg gacgtaggcc
     6841 tatttctcag gcacatgtat caagtgttcg gacgtgggtt ttcgatggtg tatcagccgc
     6901 cgccaactgg gagatgagga ggctttcttg gggggcagtc agcagttcat ttcacaagac
     6961 agaggaactt gtaaggagat gcactgattt atcttggcgc aaaccagcag gacgaattag
     7021 tgggaatagc ccgcgaatat ctaagttatg cctgtcggca tgagcagaaa cttccaattc
     7081 gaaacagttt ggagaggttg tttttgggca taccttttgt tagtcagcct ctcgattgct
     7141 catcgtcatt acacagtacc gaagtttgat cgatctagta acatagatga caccgcgcgc
     7201 gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat
     7261 tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt
     7321 attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca
     7381 ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatctgcttc gacgcactcc
     7441 ttctttactc caccatctcg tccttattga aaacgtgggt agcaccaaaa cgaatcaagt
     7501 cgctggaact gaagttacca atcacgctgg atgatttgcc agttggatta atcttgcctt
     7561 tccccgcatg aataatattg atgaatgcat gcgtgagggg tatttcgatt ttggcaatag
     7621 ctgcaattgc cgcgacatcc tccaacgagc ataattcttc agaaaaatag cgatgttcca
     7681 tgttgtcagg gcatgcatga tgcacgttat gaggtgacgg tgctaggcag tattccctca
     7741 aagtttcata gtcagtatca tattcatcat tgcattcctg caagagagaa ttgagacgca
     7801 atccacacgc tgcggcaacc ttccggcgtt cgtggtctat ttgctcttgg acgttgcaaa
     7861 cgtaagtgtt ggatcccggt cggcatctac tctattcctt tgccctcgga cgagtgctgg
     7921 ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc
     7981 ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc
     8041 atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt
     8101 ggtcaagacc aatgcggagc atatacgccc ggagccgcgg cgatcctgca agctccggat
     8161 gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga
     8221 agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg
     8281 ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt
     8341 gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg
     8401 acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg
     8461 cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga
     8521 acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catggcctcc gcgaccggct
     8581 gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc
     8641 gggagatgca ataggtcagg ctctcgctga attccccaat gtcaagcact tccggaatcg
     8701 ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc
     8761 agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt
     8821 cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact
     8881 tctcgacaga cgtcgcggtg agttcaggct ttttcatatc ggggtcgtcc tctccaaatg
     8941 aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc
     9001 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
     9061 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
     9121 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
     9181 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
     9241 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
     9301 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttgacgg atctctagga
     9361 cgcgtcgacg cgtcgacgcg tcgacgcgtc gacgcgtcct agaagctaat tcactggccg
     9421 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     9481 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     9541 aacagttgcg cagcctgaat ggcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     9601 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     9661 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
     9721 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     9781 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta
     9841 taagggattt tgccgatttc ggaaccacca tcaaacagga ttttcgcctg ctggggcaaa
     9901 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt
     9961 tgcccgtctc actggtgaaa agaaaaacca ccccagtaca ttaaaaacgt ccgcaatgtg
    10021 ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc
    10081 caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca
    10141 gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg
    10201 atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga
    10261 gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa atatcatctc
    10321 cctcgcagag atccgaatta tcagccttct tattcatttc tcgcttaacc gtgacaggct
    10381 gtcgatcttg agaactatgc cgacataata ggaaatcgct ggataaagcc gctgaggaag
    10441 ctgagtggcg ctatttcttt agaagtgaac gttgacgata tcaactcccc tatccattgc
    10501 tcaccgaatg gtacaggtcg gggacccgaa gttccgactg tcggcctgat gcatccccgg
    10561 ctgatcgacc ccagatctgg ggctgagaaa gcccagtaag gaaacaactg taggttcgag
    10621 tcgcgagatc ccccggaacc aaaggaagta ggttaaaccc gctccgatca ggccgagcca
    10681 cgccaggccg agaacattgg ttcctgtagg catcgggatt ggcggatcaa acactaaagc
    10741 tactggaacg agcagaagtc ctccggccgc cagttgccag gcggtaaagg tgagcagagg
    10801 cacgggaggt tgccacttgc gggtcagcac ggttccgaac gccatggaaa ccgcccccgc
    10861 caggcccgct gcgacgccga caggatctag cgctgcgttt ggtgtcaaca ccaacagcgc
    10921 cacgcccgca gttccgcaaa tagcccccag gaccgccatc aatcgtatcg ggctacctag
    10981 cagagcggca gagatgaaca cgaccatcag cggctgcaca gcgcctaccg tcgccgcgac
    11041 cccgcccggc aggcggtaga ccgaaataaa caacaagctc cagaatagcg aaatattaag
    11101 tgcgccgagg atgaagatgc gcatccacca gattcccgtt ggaatctgtc ggacgatcat
    11161 cacgagcaat aaacccgccg gcaacgcccg cagcagcata ccggcgaccc ctcggcctcg
    11221 ctgttcgggc tccacgaaaa cgccggacag atgcgccttg tgagcgtcct tggggccgtc
    11281 ctcctgtttg aagaccgaca gcccaatgat ctcgccgtcg atgtaggcgc cgaatgccac
    11341 ggcatctcgc aaccgttcag cgaacgcctc catgggcttt ttctcctcgt gctcgtaaac
    11401 ggacccgaac atctctggag ctttcttcag ggccgacaat cggatctcgc ggaaatcctg
    11461 cacgtcggcc gctccaagcc gtcgaatctg agccttaatc acaattgtca attttaatcc
    11521 tctgtttatc ggcagttcgt agagcgcgcc gtgcgtcccg agcgatactg agcgaagcaa
    11581 gtgcgtcgag cagtgcccgc ttgttcctga aatgccagta aagcgctggc tgctgaaccc
    11641 ccagccggaa ctgaccccac aaggccctag cgtttgcaat gcaccaggtc atcattgacc
    11701 caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg
    11761 cgccacttct tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt ttccagcttg
    11821 agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac
    11881 agcttgcggt acttctccca tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg
    11941 atttcctcgt cgatcaggac ctggcaacgg gacgttttct tgccacggtc caggacgcgg
    12001 aagcggtgca gcagcgacac cgattccagg tgcccaacgc ggtcggacgt gaagcccatc
    12061 gccgtcgcct gtaggcgcga caggcattcc tcggccttcg tgtaataccg gccattgatc
    12121 gaccagccca ggtcctggca aagctcgtag aacgtgaagg tgatcggctc gccgataggg
    12181 gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc
    12241 tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc
    12301 agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg
    12361 tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga aagctgcatt
    12421 tccttgatct gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc gctgacctgt
    12481 tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg
    12541 atggtcatcg acttcgccaa acctgccgcc tcctgttcga gacgacgcga acgctccacg
    12601 gcggccgatg gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg
    12661 gccgtagctt gctggaccat cgagccgacg gactggaagg tttcgcgggg cgcacgcatg
    12721 acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc gatcagttct
    12781 tgcctgtatg ccttccggtc aaacgtccga ttcattcacc ctccttgcgg gattgccccg
    12841 actcacgccg gggcaatgtg cccttattcc tgatttgacc cgcctggtgc cttggtgtcc
    12901 agataatcca ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc gtccttctcg
    12961 tacttggtat tccgaatctt gccctgcacg aataccagcg accccttgcc caaatacttg
    13021 ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt gcgctcctgc
    13081 ttgtcgccgg catcgttgcg ccacatctag gtactaaaac aattcatcca gtaaaatata
    13141 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
    13201 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
    13261 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
    13321 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
    13381 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc
    13441 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
    13501 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
    13561 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
    13621 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
    13681 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
    13741 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
    13801 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta
    13861 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
    13921 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
    13981 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
    14041 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc acaattatgg
    14101 gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct
    14161 tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
    14221 agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag
    14281 ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat
    14341 ggcgttggat gccgggcaac agcccgcatt atgggcgttg gcctcaacac gattttacgt
    14401 cacttaaaaa actcaggccg cagtcggtaa cctcgcgcat acagccgggc agtgacgtca
    14461 tcgtctgcgc ggaaatggac gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct
    14521 ggctgtttta cgcgtatgac agtctccgga agacggttgt tgcgcacgta ttcggtgaac
    14581 gcactatggc gacgctgggg cgtcttatga gcctgctgtc accctttgac gtggtgatat
    14641 ggatgacgga tggctggccg ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca
    14701 gcaagcgata tacgcagcga attgagcggc ataacctgaa tctgaggcag cacctggcac
    14761 ggctgggacg gaagtcgctg tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg
    14821 ggcattatct gaacataaaa cactatcaat aagttggagt cattacccaa ttatgataga
    14881 atttacaagc tataaggtta ttgtcctggg tttcaagcat tagtccatgc aagtttttat
    14941 gctttgccca ttctatagat atattgataa gcgcgctgcc tatgccttgc cccctgaaat
    15001 ccttacatac ggcgatatct tctatataaa agatatatta tcttatcagt attgtcaata
    15061 tattcaaggc aatctgcctc ctcatcctct tcatcctctt cgtcttggta gctttttaaa
    15121 tatggcgctt catagagtaa ttctgtaaag gtccaattct cgttttcata cctcggtata
    15181 atcttaccta tcacctcaaa tggttcgctg ggtttatcgc acccccgaac acgagcacgg
    15241 cacccgcgac cactatgcca agaatgccca aggtaaaaat tgccggcccc gccatgaagt
    15301 ccgtgaatgc cccgacggcc gaagtgaagg gcaggccgcc acccaggccg ccgccctcac
    15361 tgcccggcac ctggtcgctg aatgtcgatg ccagcacctg cggcacgtca atgcttccgg
    15421 gcgtcgcgct cgggctgatc gcccatcccg ttactgcccc gatcccggca atggcaagga
    15481 ctgccagcgc tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg
    15541 gggatgggag gcccgcgtta gcgggccggg agggttcgag aagggggggc accccccttc
    15601 ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt tataaatatt
    15661 ggtttaaaag caggttaaaa gacaggttag cggtggccga aaaacgggcg gaaacccttg
    15721 caaatgctgg attttctgcc tgtggacagc ccctcaaatg tcaataggtg cgcccctcat
    15781 ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg
    15841 cccctcaagt gtcaataccg cagggcactt atccccaggc ttgtccacat catctgtggg
    15901 aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg ctggccagct ccacgtcgcc
    15961 ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa
    16021 gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt tttccgcgag gtatccacaa
    16081 cgccggcggc cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg
    16141 ccatagacgg ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt cggaaaggcg
    16201 c
//
