LOCUS       sequence-247674-        2414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-Prrn (-64 to +17)
FEATURES             Location/Qualifiers
     source          1..2414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(620..637)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(730..1318)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(810..829)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1492..2286)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2287..2391)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccag agctcggtct caggagatga gaatggataa gaggctcgtg
      421 ggattgacgt gagggggcag ggatggctat atttctggga gcgaactccg ggcgaattac
      481 ttgagaccgg tacctggagc acaagactgg cctcatgggc cttcctttca ctgcccgctt
      541 tccagtcggg aaacctgtcg tgccagctgc attaacatgg tcatagctgt ttccttgcgt
      601 attgggcgct ctccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cgggtaaagc
      661 ctggggtgcc taatgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      721 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      781 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      841 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      901 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      961 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1021 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1081 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1141 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1201 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1261 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1321 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1381 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1441 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt attagaaaaa
     1501 ttcatccagc agacgataaa acgcaatacg ctggctatcc ggtgccgcaa tgccatacag
     1561 caccagaaaa cgatccgccc attcgccgcc cagttcttcc gcaatatcac gggtggccag
     1621 cgcaatatcc tgataacgat ccgccacgcc cagacggccg caatcaataa agccgctaaa
     1681 acggccattt tccaccataa tgttcggcag gcacgcatca ccatgggtca ccaccagatc
     1741 ttcgccatcc ggcatgctcg ctttcagacg cgcaaacagc tctgccggtg ccaggccctg
     1801 atgttcttca tccagatcat cctgatccac caggcccgct tccatacggg tacgcgcacg
     1861 ttcaatacga tgtttcgcct gatgatcaaa cggacaggtc gccgggtcca gggtatgcag
     1921 acgacgcatg gcatccgcca taatgctcac tttttctgcc ggcgccagat ggctagacag
     1981 cagatcctga cccggcactt cgcccagcag cagccaatca cggcccgctt cggtcaccac
     2041 atccagcacc gccgcacacg gaacaccggt ggtggccagc cagctcagac gcgccgcttc
     2101 atcctgcagc tcgttcagcg caccgctcag atcggttttc acaaacagca ccggacgacc
     2161 ctgcgcgctc agacgaaaca ccgccgcatc agagcagcca atggtctgct gcgcccaatc
     2221 atagccaaac agacgttcca cccacgctgc cgggctaccc gcatgcaggc catcctgttc
     2281 aatcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2341 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     2401 ccgaaaagtg ccac
//
