LOCUS       sequence-247706-        2185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB part for pOR1-OR2 (lambda 
            cI-repressable strong promoter) - Please see Supplemental Documents 
            for annotated Genbank file.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2185)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P9m
FEATURES             Location/Qualifiers
     source          1..2185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..27
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(31..74)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    77..98
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    205..225
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(231..248)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(402..990)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(482..501)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1161..1952,1953..2021))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1784..1803
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2022..2126)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg
       61 ctaaggatga tttctggaat tcgcggccgc ttctagagac tagtgggtct caggagtgct
      121 gttccgctgg gcatgctgag ctaacaccgt gcgtgttgac aattttacct ctggcggtga
      181 taatggttgc agctactaga gacctactag tagcggccgc tgcaggcttc ctcgctcact
      241 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      301 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      361 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc
      421 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      481 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      541 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      601 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      661 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      721 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      781 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      841 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      901 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      961 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1021 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     1081 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1141 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     1201 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     1261 gagggcttac catctggccc cagtgctgca atgataccgc gcgacccacg ctcaccggct
     1321 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1381 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1441 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1501 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1561 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     1621 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     1681 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     1741 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     1801 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     1861 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     1921 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1981 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     2041 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2101 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     2161 gaaaccatta ttatcatgac attaa
//
