LOCUS       sequence-247974-        2447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-rrn16 5'UTR (native)
FEATURES             Location/Qualifiers
     source          1..2447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(653..670)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(763..1351)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(843..862)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1525..2319)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2320..2424)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccag agctcggtct catactgcga actccgggcg aatacgaagc
      421 gcatggatac aagttatgcc ttggaatgaa agacaattcc gaatccgctt tgtctacgaa
      481 caaggaagct ataagtaatg caactatgaa aatgtgagac cggtacctgg agcacaagac
      541 tggcctcatg ggccttcctt tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
      601 tgcattaaca tggtcatagc tgtttccttg cgtattgggc gctctccgct tcctcgctca
      661 ctgactcgct gcgctcggtc gttcgggtaa agcctggggt gcctaatgag caaaaggcca
      721 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      781 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      841 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      901 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      961 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1021 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1081 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1141 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1201 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1261 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1321 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1381 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1441 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1501 tgagtaaact tggtctgaca gttattagaa aaattcatcc agcagacgat aaaacgcaat
     1561 acgctggcta tccggtgccg caatgccata cagcaccaga aaacgatccg cccattcgcc
     1621 gcccagttct tccgcaatat cacgggtggc cagcgcaata tcctgataac gatccgccac
     1681 gcccagacgg ccgcaatcaa taaagccgct aaaacggcca ttttccacca taatgttcgg
     1741 caggcacgca tcaccatggg tcaccaccag atcttcgcca tccggcatgc tcgctttcag
     1801 acgcgcaaac agctctgccg gtgccaggcc ctgatgttct tcatccagat catcctgatc
     1861 caccaggccc gcttccatac gggtacgcgc acgttcaata cgatgtttcg cctgatgatc
     1921 aaacggacag gtcgccgggt ccagggtatg cagacgacgc atggcatccg ccataatgct
     1981 cactttttct gccggcgcca gatggctaga cagcagatcc tgacccggca cttcgcccag
     2041 cagcagccaa tcacggcccg cttcggtcac cacatccagc accgccgcac acggaacacc
     2101 ggtggtggcc agccagctca gacgcgccgc ttcatcctgc agctcgttca gcgcaccgct
     2161 cagatcggtt ttcacaaaca gcaccggacg accctgcgcg ctcagacgaa acaccgccgc
     2221 atcagagcag ccaatggtct gctgcgccca atcatagcca aacagacgtt ccacccacgc
     2281 tgccgggcta cccgcatgca ggccatcctg ttcaatcata ctcttccttt ttcaatatta
     2341 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2401 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
