LOCUS       sequence-247976-        2355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-rrn16 5'UTR (+37)
FEATURES             Location/Qualifiers
     source          1..2355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(561..578)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(671..1259)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(751..770)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1433..2227)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2228..2332)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatc aacgagctcg gtctcatact gcgaactccg ggcgaatacg aagcgcatgg
      421 atacaagaat gtgagaccgg taccctctgg gcctcatggg ccttcctttc actgcccgct
      481 ttccagtcgg gaaacctgtc gtgccagctg cattaacatg gtcatagctg tttccttgcg
      541 tattgggcgc tctccgcttc ctcgctcact gactcgctgc gctcggtcgt tcgggtaaag
      601 cctggggtgc ctaatgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      661 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      721 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      781 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      841 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
      901 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
      961 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1021 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1081 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1141 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1201 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1261 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1321 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1381 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt tattagaaaa
     1441 attcatccag cagacgataa aacgcaatac gctggctatc cggtgccgca atgccataca
     1501 gcaccagaaa acgatccgcc cattcgccgc ccagttcttc cgcaatatca cgggtggcca
     1561 gcgcaatatc ctgataacga tccgccacgc ccagacggcc gcaatcaata aagccgctaa
     1621 aacggccatt ttccaccata atgttcggca ggcacgcatc accatgggtc accaccagat
     1681 cttcgccatc cggcatgctc gctttcagac gcgcaaacag ctctgccggt gccaggccct
     1741 gatgttcttc atccagatca tcctgatcca ccaggcccgc ttccatacgg gtacgcgcac
     1801 gttcaatacg atgtttcgcc tgatgatcaa acggacaggt cgccgggtcc agggtatgca
     1861 gacgacgcat ggcatccgcc ataatgctca ctttttctgc cggcgccaga tggctagaca
     1921 gcagatcctg acccggcact tcgcccagca gcagccaatc acggcccgct tcggtcacca
     1981 catccagcac cgccgcacac ggaacaccgg tggtggccag ccagctcaga cgcgccgctt
     2041 catcctgcag ctcgttcagc gcaccgctca gatcggtttt cacaaacagc accggacgac
     2101 cctgcgcgct cagacgaaac accgccgcat cagagcagcc aatggtctgc tgcgcccaat
     2161 catagccaaa cagacgttcc acccacgctg ccgggctacc cgcatgcagg ccatcctgtt
     2221 caatcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     2281 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     2341 cccgaaaagt gccac
//
