LOCUS       sequence-248014-        3911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Terminator acceptor vector. Minimal cargo vector; with eGFP in 
            downstream secondary site and constitutive promoter J23100 in 
            upstream site. Allows introducing any part using BsmBI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 13 to 47)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-Tac
FEATURES             Location/Qualifiers
     source          1..3911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..47
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     misc_feature    101..122
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    144..164
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             192..212
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             232..939
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     CDS             232..939
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(256..284)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     879..899
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      979..1073
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1146..1237
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1238..1306,1307..2098)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2102..2141
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2288..2396
                     /note="incP origin of transfer"
     rep_origin      2432..3020
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2921..2940
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3174..3191
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3206..3346
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3272..3291)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3432..3454
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3448..3639)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3819..3905)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttgacggc tagctcagtc ctaggtacag tgctagcgta cttaacttct
       61 gactgagttg cacgcttaac ttctgactga gttgcacgct gaattcgcgg ccgcttctag
      121 agggtcttga gacgtctctg acctactagt agcggccgct gcagggagta acttctgact
      181 gagttgcata cttgtttaac tttaagaagg agatatacaa tggtagcccg taaaggagaa
      241 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
      301 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
      361 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt
      421 tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag
      481 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
      541 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
      601 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat
      661 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc
      721 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
      781 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
      841 gccctttcga aagatcccaa cgaaaagaga gatcacatgg tccttcttga gtttgtaaca
      901 gctgctggga ttacacatgg catggatgaa ctatacaaag gttcgtaact tctgactgag
      961 ttgcacgctc tagtcttgga ctcctgttga tagatccagt aatgacctca gaactccatc
     1021 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccagggg
     1081 tccccaataa ttacgattta aattagtagc ccgcctaatg agcgggcttt tttttaattc
     1141 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     1201 ctgataaatg cttcaataat attgaaaaag gaagagtatg agcattcagc attttcgtgt
     1261 ggcgctgatt ccgttttttg cggcgttttg cctgccggtg tttgcgcatc cggaaaccct
     1321 ggtgaaagtg aaagatgcgg aagatcaact gggtgcgcgc gtgggctata ttgaactgga
     1381 tctgaacagc ggcaaaattc tggaatcttt tcgtccggaa gaacgttttc cgatgatgag
     1441 cacctttaaa gtgctgctgt gcggtgcggt tctgagccgt gtggatgcgg gccaggaaca
     1501 actgggccgt cgtattcatt atagccagaa cgatctggtg gaatatagcc cggtgaccga
     1561 aaaacatctg accgatggca tgaccgtgcg tgaactgtgc agcgcggcga ttaccatgag
     1621 cgataacacc gcggcgaacc tgctgctgac gaccattggc ggtccgaaag aactgaccgc
     1681 gtttctgcat aacatgggcg atcatgtgac ccgtctggat cgttgggaac cggaactgaa
     1741 cgaagcgatt ccgaacgatg aacgtgatac caccatgccg gcagcaatgg cgaccaccct
     1801 gcgtaaactg ctgacgggtg agctgctgac cctggcaagc cgccagcaac tgattgattg
     1861 gatggaagcg gataaagtgg cgggtccgct gctgcgtagc gcgctgccgg ctggctggtt
     1921 tattgcggat aaaagcggtg cgggcgaacg tggcagccgt ggcattattg cggcgctggg
     1981 cccggatggt aaaccgagcc gtattgtggt gatttatacc accggcagcc aggcgacgat
     2041 ggatgaacgt aaccgtcaga ttgcggaaat tggcgcgagc ctgattaaac attggtaaac
     2101 cgatacaatt aaaggctcct tttggagcct ttttttttgg acgacccttg tccttttccg
     2161 ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg
     2221 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc
     2281 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
     2341 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg
     2401 ccggccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2461 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2521 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2581 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2641 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2701 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2761 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2821 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2881 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2941 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3001 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3061 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3121 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3181 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     3241 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     3301 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     3361 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     3421 tgtgacagtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     3481 gaggcagctg cggtaaagct catcagcgtg gtcgtgcagc gattcacaga tgtctgcctg
     3541 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
     3601 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
     3661 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt
     3721 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg
     3781 gatgcggcgg gggcgcgccc agctgtctag ggcggcggat ttgtcctact caggagagcg
     3841 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
     3901 atttgatgcc t
//
