LOCUS       sequence-24802-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Groth AC, Olivares EC, Thyagarajan B, Calos MP
  TITLE     A phage integrase directs efficient site-specific integration in 
            human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 May 23. 97(11):5995-6000.
  PUBMED    10801973
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2000 May 23. 97(11):5995-6000."
COMMENT     Sequence Label: pTA-attP
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    125..224
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     primer_bind     complement(356..375)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(357..375)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(382..399)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(382..398)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(391..413)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(626..645)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actggtcgag ctggatcact agtaacggcc gccagtgtgc tggaattcgg cttcgactag
       61 tactgacgga cacaccgaag ccccggcggc aaccctcagc ggatgccccg gggcttcacg
      121 ttttcccagg tcagaagcgg ttttcgggag tagtgcccca actggggtaa cctttgagtt
      181 ctctcagttg ggggcgtagg gtcgccgaca tgacacaagg ggttgtgacc ggggtggaca
      241 cgtacgcggg tgcttacgac cgtcagtcgc gcgagcgcga ctagtacaag ccgaattctg
      301 cagatatcca tcacactggc ggccgctcga gcatgcatct agagggccca attcgcccta
      361 tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      421 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      481 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac
      541 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
      601 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      661 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt cnaatttagt
      721 gctttnggna cctcganccc aaaaattgat tanggtgatg ntcacgtagt ggncatcgcc
      781 tgaaagtntt tccccntnga gtggancnct tnttaaagga antttgtcaa ngnanaactc
      841 accttntgt
//
