LOCUS       sequence-248070-        6524 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Abscisic acid-inducible FUT8 expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Chang MM, Gaidukov L, Jung G, Tseng WA, Scarcelli JJ, Cornell R, 
            Marshall JK, Lyles JL, Sakorafas P, Chu AA, Cote K, Tzvetkova B, 
            Dolatshahi S, Sumit M, Mulukutla BC, Lauffenburger DA, Figueroa B 
            Jr, Summers NM, Lu TK, Weiss R
  TITLE     Small-molecule control of antibody N-glycosylation in engineered 
            mammalian cells.
  JOURNAL   Nat Chem Biol. 2019 May 20. pii: 10.1038/s41589-019-0288-4. doi: 
            10.1038/s41589-019-0288-4.
  PUBMED    31110306
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2019 May 20. pii: 10.1038/s41589-019-0288-4. doi: 
            10.1038/s41589-019-0288-4."
COMMENT     Sequence Label: FUT8-ABA
FEATURES             Location/Qualifiers
     source          1..6524
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    388..1224
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(1334..1353)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1345..2154
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1412..1431)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(2407..2995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2487..2506)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3166..3957,3958..4026))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3789..3808
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4027..4131)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4157..4612)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4294..4315)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4506..4525
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4739..4761
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4753..4770
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4754..4770
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4780..4799
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4780..4798
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4844..4864
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4882..4919
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    4929..4953
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4963..4972
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4969..5367
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    complement(5369..5393)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5483..5502)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5548..5603
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5602..5621)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5964..5980)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5964..5980)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5977..5999)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5988..6004
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6012..6018,6019..6036,6037..6042))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6057..6078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggcgcgcctg tcattctaaa tctctctttc agcctaaagc tttttccccg tatcccccca
       61 ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc
      121 ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg
      181 accggagcgg agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac
      241 atccctgggg gctttggggg ggggctgtcc ccgtgagctc ccaggcgcgc caactcggta
      301 ccgtatcgat gtcgacggtg cgtttttatg cttgtagtat tgtataatgt ttttaagatc
      361 ctagggataa cagggtaatc ggccgctact agggacagga ttggtgacag aaaagcccca
      421 tccttaggcc tcctccttcc tagtctcctg atattgggtc taacccccac ctcctgttag
      481 gcagattcct tatctggtga cacaccccca tttcctggag ccatctctct ccttgccaga
      541 acctctaagg tttgcttacg atggagccag agaggatcct gggagggaga gcttggcagg
      601 gggtgggagg gaaggggggg atgcgtgacc tgcccggttc tcagtggcca ccctgcgcta
      661 ccctctccca gaacctgagc tgctctgacg cggctgtctg gtgcgtttca ctgatcctgg
      721 tgctgcagct tccttacact tcccaagagg agaagcagtt tggaaaaaca aaatcagaat
      781 aagttggtcc tgagttctaa ctttggctct tcacctttct agtccccaat ttatattgtt
      841 cctccgtgcg tcagttttac ctgtgagata aggccagtag ccagccccgt cctggcaggg
      901 ctgtggtgag gaggggggtg tccgtgtgga aaactccctt tgtgagaatg gtgcgtccta
      961 ggtgttcacc aggtcgtggc cgcctctact ccctttctct ttctccatcc ttctttcctt
     1021 aaagagtccc cagtgctatc tgggacatat tcctccgccc agagcagggt cccgcttccc
     1081 taaggccctg ctctgggctt ctgggtttga gtccttggca agcccaggag aggcgctcag
     1141 gcttccctgt cccccttcct cgtccaccat ctcatgcccc tggctctcct gccccttccc
     1201 tacaggggtt cctggctctg ctctccaccg cgggagctct ggcccgtgtc tcaaaatctc
     1261 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     1321 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     1381 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     1441 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     1501 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     1561 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     1621 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     1681 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     1741 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     1801 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     1861 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     1921 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     1981 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     2041 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     2101 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     2161 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     2221 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agacggtatc
     2281 actagtatta cagaggtaag ttataacagt cgcctaaccc aattgagggg ataacgcagg
     2341 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2401 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2461 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2521 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2581 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2641 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2701 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2761 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2821 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2881 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2941 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3001 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3061 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3121 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3181 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3241 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3301 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3361 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3421 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3481 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3541 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3601 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3661 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3721 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3781 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3841 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3901 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3961 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     4021 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4081 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4141 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     4201 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     4261 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     4321 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     4381 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     4441 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     4501 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     4561 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     4621 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     4681 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     4741 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc
     4801 gaattgggta ccgtatcgat gtcgacgtta acgctagtga tatcaacttt gtatagaaaa
     4861 gttggctccg aatttctcga gggcttgtcg acgacggcgg actccgtcgt caggatcatg
     4921 aattcgaccc aagtttgtac aaaaaagcag gctgaacggc ctgccaccat ggccaagcct
     4981 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
     5041 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
     5101 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
     5161 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
     5221 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
     5281 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
     5341 ccctctggtt atgtgtggga gggctaatac ccagctttct tgtacaaagt ggtacgcgtg
     5401 aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     5461 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
     5521 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     5581 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     5641 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
     5701 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     5761 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
     5821 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     5881 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
     5941 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     6001 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     6061 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     6121 tcgtgccagc ggatcgacag tactaagctt ggttgcttgc aaaagcagta attggaaagc
     6181 actctcaaag aatcccaatt gcatcgatgg taccagtgaa agtctgcaat gaattcgagt
     6241 tggcgcgcct gtcattctaa atctctcttt cagcctaaag ctttttcccc gtatcccccc
     6301 aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat cccgtgccac
     6361 cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg
     6421 gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta
     6481 catccctggg ggctttggga gggggctgtc cccgtgagct ccca
//
