LOCUS       sequence-248148-        3913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part P- Promoter (constitutive); J23100.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 86 to 120)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-J23100_P
FEATURES             Location/Qualifiers
     source          1..3913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        86..120
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     misc_feature    146..166
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             194..214
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             234..941
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     CDS             234..941
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(258..286)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     881..901
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      981..1075
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1148..1239
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1240..1308,1309..2100)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2104..2143
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2290..2398
                     /note="incP origin of transfer"
     rep_origin      2434..3022
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2923..2942
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3176..3193
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3208..3348
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3274..3293)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3434..3456
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3450..3641)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3821..3907)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tggagttgac ggctagctca gtcctaggta cagtgctagc
      121 gtacttgaga cctttcatcg cgacctacta gtagcggccg ctgcagggag taacttctga
      181 ctgagttgca tacttgttta actttaagaa ggagatatac aatggtagcc cgtaaaggag
      241 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      301 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
      361 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg
      421 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca
      481 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
      541 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
      601 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
      661 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
      721 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
      781 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
      841 ctgccctttc gaaagatccc aacgaaaaga gagatcacat ggtccttctt gagtttgtaa
      901 cagctgctgg gattacacat ggcatggatg aactatacaa aggttcgtaa cttctgactg
      961 agttgcacgc tctagtcttg gactcctgtt gatagatcca gtaatgacct cagaactcca
     1021 tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccagg
     1081 ggtccccaat aattacgatt taaattagta gcccgcctaa tgagcgggct tttttttaat
     1141 tcccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     1201 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagcattca gcattttcgt
     1261 gtggcgctga ttccgttttt tgcggcgttt tgcctgccgg tgtttgcgca tccggaaacc
     1321 ctggtgaaag tgaaagatgc ggaagatcaa ctgggtgcgc gcgtgggcta tattgaactg
     1381 gatctgaaca gcggcaaaat tctggaatct tttcgtccgg aagaacgttt tccgatgatg
     1441 agcaccttta aagtgctgct gtgcggtgcg gttctgagcc gtgtggatgc gggccaggaa
     1501 caactgggcc gtcgtattca ttatagccag aacgatctgg tggaatatag cccggtgacc
     1561 gaaaaacatc tgaccgatgg catgaccgtg cgtgaactgt gcagcgcggc gattaccatg
     1621 agcgataaca ccgcggcgaa cctgctgctg acgaccattg gcggtccgaa agaactgacc
     1681 gcgtttctgc ataacatggg cgatcatgtg acccgtctgg atcgttggga accggaactg
     1741 aacgaagcga ttccgaacga tgaacgtgat accaccatgc cggcagcaat ggcgaccacc
     1801 ctgcgtaaac tgctgacggg tgagctgctg accctggcaa gccgccagca actgattgat
     1861 tggatggaag cggataaagt ggcgggtccg ctgctgcgta gcgcgctgcc ggctggctgg
     1921 tttattgcgg ataaaagcgg tgcgggcgaa cgtggcagcc gtggcattat tgcggcgctg
     1981 ggcccggatg gtaaaccgag ccgtattgtg gtgatttata ccaccggcag ccaggcgacg
     2041 atggatgaac gtaaccgtca gattgcggaa attggcgcga gcctgattaa acattggtaa
     2101 accgatacaa ttaaaggctc cttttggagc cttttttttt ggacgaccct tgtccttttc
     2161 cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt
     2221 cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg
     2281 gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac
     2341 tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccgta
     2401 ggccggcccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2461 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2521 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2581 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2641 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2701 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2761 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2821 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2881 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2941 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3001 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3061 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3121 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     3181 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     3241 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     3301 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     3361 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     3421 gctgtgacag tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     3481 gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgca gcgattcaca gatgtctgcc
     3541 tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata
     3601 aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg
     3661 ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg
     3721 gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta
     3781 tggatgcggc gggggcgcgc ccagctgtct agggcggcgg atttgtccta ctcaggagag
     3841 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
     3901 ttatttgatg cct
//
