LOCUS       sequence-24815-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Lundby A, Mutoh H, Dimitrov D, Akemann W, Knoepfel T
  TITLE     Engineering of a genetically encodable fluorescent voltage sensor 
            exploiting fast Ci-VSP voltage-sensing movements.
  JOURNAL   PLoS ONE. 2008  . 3(6):e2514.
  PUBMED    18575613
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE. 
            2008  . 3(6):e2514."
COMMENT     Sequence Label: pcDNA 3.1(-) VSFP3.1
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgggggcttt agcgagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa
       61 ttaatacgac tcactatagg gagacccaag ctggctagcg ccaccatgga gggattcgac
      121 ggttcagatt ttagtcctcc agctgattta gttggcgttg acggtgcagt catgcggaac
      181 gtcgttgacg tcacgataaa tggtgacgtc actgctccgc cgaaagctgc gccaagaaaa
      241 agtgaatcgg taaagaaagt tcattggaat gatgtagacc aaggaccgag tgaaaaacca
      301 gagacaagac aggaggaacg aatagatata cccgagattt caggtctatg gtggggcgag
      361 aatgaacatg gagtggacga tgggagaatg gagataccta ctactggtgt aggtcgcgtc
      421 cagtttcgtg tccgagcagt gattgatcat ctagggatgc gagtctttgg agtcttccta
      481 attttcttgg acatcatcct catgatcatt gatctcagtc ttccaggaaa aagtgaatct
      541 tcacaatcct tttatgacgg gatggctttg gctctttctt gttatttcat gctggattta
      601 ggattaagga tatttgccta cgggcccaag aatttcttca ccaacccctg ggaggttgct
      661 gatggtttga ttatcgttgt cacattcgtc gtcacgatat tttacactgt gttagatgaa
      721 tacgtgcaag aaacaggagc cgatggtttg gggcagttgg ttgtgttggc ccgtttgctg
      781 cgtgtggttc gattagcaag aatattttat tcccatcaca atgcggccgc atggtg
//
