LOCUS       sequence-248242-       10118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Negative control for ATP measurement, Tol2 transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Yaginuma H, Kawai S, Tabata KV, Tomiyama K, Kakizuka A, Komatsuzaki 
            T, Noji H, Imamura H
  TITLE     Diversity in ATP concentrations in a single bacterial cell 
            population revealed by quantitative single-cell imaging.
  JOURNAL   Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038/srep06522.
  PUBMED    25283467
REFERENCE   2  (bases 1 to 10118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-10-6"; volume: "4:6522. doi"; pages: " 
            10.1038/srep06522"
COMMENT     Sequence Label: pT2MP.EF-QNA
FEATURES             Location/Qualifiers
     source          1..10118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(655..666,667..854)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    2123..2171
                     /note="synthetic polyadenylation signal"
     promoter        2206..3388
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2437..3379
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3336..3356
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3434..3443
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3800..4051
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     3988..4009
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4115..4136)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4376..4395)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    4642..4866
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4913..4936)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5310..5329
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5394..5993
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5394..5413)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5890..5910
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6080..6304
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6332..6348)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6356..6372
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6380..6386,6387..6404,6405..6410))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6425..6446
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    join(7762..7899,7900..7911)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(7930..7948)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7969..7985)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7993..8009
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8017..8023,8024..8041,8042..8047))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8062..8083
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8200..8217)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8371..8959)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8451..8470)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9130..9921,9922..9990))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9753..9772
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9991..10095)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccagagg
      661 tgtaaagtac ttgagtaatt ttacttgatt actgtactta agtattattt ttggggattt
      721 ttactttact tgagtacaat taaaaatcaa tacttttact tttacttaat tacatttttt
      781 tagaaaaaaa agtacttttt actccttaca attttattta cagtcaaaaa gtacttattt
      841 tttggagatc acttgtcgag tagggataac agggtaatga tcccccgggc tgcagtcgcg
      901 acgcgtccta gagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc
      961 ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca
     1021 tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct
     1081 ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa
     1141 aaagctttag gctgaaagag agatttagaa tgacagaatc atagaacggc ctgggttgca
     1201 aaggagcaca gtgctcatcc agatccaacc ccctgctatg tgcagggtca tcaaccagca
     1261 gcccaggctg cccagagcca catccagcct ggccttgaat gcctgcaggg atggggcatc
     1321 cacagcctcc ttgggcaacc tgttcagtgc gtcaccaccc tctgggggaa aaactgcctc
     1381 ctcatatcca acccaaacct cccctgtctc agtgtaaagc cattccccct tgtcctatca
     1441 agggggagtt tgctgtgaca ttgttggtct ggggtgacac atgtttgcca attcagtgca
     1501 tcacggagag gcagatcttg gggataagga agtgcaggac agcatggacg tgggacatgc
     1561 aggtgttgag ggctctggga cactctccaa gtcacagcgt tcagaacagc cttaaggata
     1621 agaagatagg atagaaggac aaagagcaag ttaaaaccca gcatggagag gagcacaaaa
     1681 aggccacaga cactgctggt ccctgtgtct gagcctgcat gtttgatggt gtctggatgc
     1741 aagcagaagg ggtggaagag cttgcctgga gagatacagc tgggtcagta ggactgggac
     1801 aggcagctgg agaattgcca tgtagatgtt catacaatcg tcaaatcatg aaggctggaa
     1861 aagccctcca agatccccaa gaccaacccc aacccaccca ccgtgcccac tggccatgtc
     1921 cctcagtgcc acatccccac agttcttcat cacctccagg gacggtgacc cccccacctc
     1981 cgtgggcagc tgtgccactg cagcaccgct ctttggagaa ggtaaatctt gctaaatcca
     2041 gcccgaccct cccctggcac aacgtaaggc cattatctct catccaactc caggacggag
     2101 tcagtgagga tggggctcta ggaataaaat atctttattt tcattacatc tgtgtgttgg
     2161 ttttttgtgt ggtcgacatt gattattgac tagatcatcg cgtgaggctc cggtgcccgt
     2221 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     2281 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     2341 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     2401 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     2461 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacgcccctg
     2521 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag
     2581 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg
     2641 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc
     2701 tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct
     2761 tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga
     2821 cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc
     2881 gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc
     2941 gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc
     3001 ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc
     3061 gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt
     3121 cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag
     3181 cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca
     3241 cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga
     3301 atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag
     3361 tttttttctt ccatttcagg tgtcgtgagg aattctgcag tcgacggtac cgcgggcccg
     3421 ggatccaccg gtcgccacca tggtgaaaac tgtgaaagtg aatataacaa cccctgatgg
     3481 gccagtctac gacgctgata tcgagatggt gtccgtgcgg gccgagagtg gtgatctcgg
     3541 catcctcccc ggtcacattc ccacaaaggc cccactgaag atcggagctg tgcggctgaa
     3601 gaaggacggc caaaccgaga tggtcgcagt ctcaggcggc actgttgaag tgcggcctga
     3661 ccacgttacc attaatgctc aagccgctga aacagccgaa ggaatcgaca aagagagagc
     3721 agaagccgca agacagaggg cccaggagcg gctgaactct caaacccgtt acaacagcca
     3781 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     3841 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     3901 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
     3961 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     4021 gatcactctc ggcatggacg agctgtacaa gggtggcacc ggagggagca tggtgagcaa
     4081 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     4141 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     4201 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     4261 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     4321 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     4381 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4441 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     4501 caacctgggt gacaccgata ttcgccgggc cgagctggca ctgcagaagg ccctgaacaa
     4561 gctggacgtg gctgggaagg caaactgagc ggccgctcga taagcttgat cgatgatcta
     4621 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4681 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4741 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4801 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     4861 ctatggcttc tgaggcggaa agaacctgca gcttttccca aggcagtctg gagcatgcgc
     4921 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     4981 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     5041 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     5101 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     5161 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     5221 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     5281 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     5341 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgtggccaca accatgaccg
     5401 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     5461 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     5521 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     5581 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     5641 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     5701 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     5761 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     5821 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     5881 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     5941 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
     6001 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg atgatctaga
     6061 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     6121 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     6181 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     6241 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     6301 atggctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6361 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6421 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6481 gaaacctgtc gtgccagcgg atccctagag ggacagcccc cccccaaagc ccccagggat
     6541 gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt
     6601 ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa
     6661 ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc
     6721 tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga cagaatcata
     6781 gaacggcctg ggttgcaaag gagcacagtg ctcatccaga tccaaccccc tgctatgtgc
     6841 agggtcatca accagcagcc caggctgccc agagccacat ccagcctggc cttgaatgcc
     6901 tgcagggatg gggcatccac agcctccttg ggcaacctgt tcagtgcgtc accaccctct
     6961 gggggaaaaa ctgcctcctc atatccaacc caaacctccc ctgtctcagt gtaaagccat
     7021 tcccccttgt cctatcaagg gggagtttgc tgtgacattg ttggtctggg gtgacacatg
     7081 tttgccaatt cagtgcatca cggagaggca gatcttgggg ataaggaagt gcaggacagc
     7141 atggacgtgg gacatgcagg tgttgagggc tctgggacac tctccaagtc acagcgttca
     7201 gaacagcctt aaggataaga agataggata gaaggacaaa gagcaagtta aaacccagca
     7261 tggagaggag cacaaaaagg ccacagacac tgctggtccc tgtgtctgag cctgcatgtt
     7321 tgatggtgtc tggatgcaag cagaaggggt ggaagagctt gcctggagag atacagctgg
     7381 gtcagtagga ctgggacagg cagctggaga attgccatgt agatgttcat acaatcgtca
     7441 aatcatgaag gctggaaaag ccctccaaga tccccaagac caaccccaac ccacccaccg
     7501 tgcccactgg ccatgtccct cagtgccaca tccccacagt tcttcatcac ctccagggac
     7561 ggtgaccccc ccacctccgt gggcagctgt gccactgcag caccgctctt tggagaaggt
     7621 aaatcttgct aaatccagcc cgaccctccc ctggcacaac gtaaggccat tatctctcat
     7681 ccaactccag gacggagtca gtgaggatgg ggctctagcc ggaccgacgc gtctcgatag
     7741 ggataacagg gtaattgatc caatactcaa gtacaatttt aatggagtac ttttttactt
     7801 ttactcaagt aagattctag ccagatactt ttacttttaa ttgagtaaaa ttttccctaa
     7861 gtacttgtac tttcacttga gtaaaatttt tgagtacttt ttacacctct ggagctccag
     7921 cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt
     7981 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8041 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8101 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8161 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8221 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8281 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8341 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8401 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8461 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8521 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8581 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8641 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8701 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8761 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     8821 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8881 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8941 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9001 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9061 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9121 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9181 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9241 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9301 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9361 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9421 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9481 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9541 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9601 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9661 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9721 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9781 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9841 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9901 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9961 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10021 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10081 aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
