LOCUS       sequence-248371-        3353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part O- ORF; sfGFP; fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP18-sfGFP_O
FEATURES             Location/Qualifiers
     source          1..3353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             86..796
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     misc_feature    823..843
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      877..971
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1044..1135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1136..1204,1205..1996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2000..2039
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2186..2294
                     /note="incP origin of transfer"
     rep_origin      2330..2918
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2819..2838
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3072..3089
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3206..3227
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3261..3347)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tagcccgtaa aggcgaagag ctgttcactg gtgtcgtccc
      121 tattctggtg gaactggatg gtgatgtcaa cggtcataag ttttccgtgc gtggcgaggg
      181 tgaaggtgac gcaactaatg gtaaactgac gctgaagttc atctgtacta ctggtaaact
      241 gccggtacct tggccgactc tggtaacgac gctgacttat ggtgttcagt gctttgctcg
      301 ttatccggac catatgaagc agcatgactt cttcaagtcc gccatgccgg aaggctatgt
      361 gcaggaacgc acgatttcct ttaaggatga cggcacgtac aaaacgcgtg cggaagtgaa
      421 atttgaaggc gataccctgg taaaccgcat tgagctgaaa ggcattgact ttaaagaaga
      481 tggcaatatc ctgggccata agctggaata caattttaac agccacaatg tttacatcac
      541 cgccgataaa caaaaaaatg gcattaaagc gaattttaaa attcgccaca acgtggagga
      601 tggcagcgtg cagctggctg atcactacca gcaaaacact ccaatcggtg atggtcctgt
      661 tctgctgcca gacaatcact atctgagcac gcaaagcgtt ctgtctaaag atccgaacga
      721 gaaacgcgat catatggttc tgctggagtt cgtaaccgca gcgggcatca cgcatggtat
      781 ggatgaactg tacaaaggtt cgtgagacct ttcatcgcga cctactagta gcggccgctg
      841 cagggagttg tcttcgaaga cttcgctcta gtcttggact cctgttgata gatccagtaa
      901 tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta
      961 ttggtgagaa tccaggggtc cccaataatt acgatttaaa ttagtagccc gcctaatgag
     1021 cgggcttttt tttaattccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     1081 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     1141 cattcagcat tttcgtgtgg cgctgattcc gttttttgcg gcgttttgcc tgccggtgtt
     1201 tgcgcatccg gaaaccctgg tgaaagtgaa agatgcggaa gatcaactgg gtgcgcgcgt
     1261 gggctatatt gaactggatc tgaacagcgg caaaattctg gaatcttttc gtccggaaga
     1321 acgttttccg atgatgagca cctttaaagt gctgctgtgc ggtgcggttc tgagccgtgt
     1381 ggatgcgggc caggaacaac tgggccgtcg tattcattat agccagaacg atctggtgga
     1441 atatagcccg gtgaccgaaa aacatctgac cgatggcatg accgtgcgtg aactgtgcag
     1501 cgcggcgatt accatgagcg ataacaccgc ggcgaacctg ctgctgacga ccattggcgg
     1561 tccgaaagaa ctgaccgcgt ttctgcataa catgggcgat catgtgaccc gtctggatcg
     1621 ttgggaaccg gaactgaacg aagcgattcc gaacgatgaa cgtgatacca ccatgccggc
     1681 agcaatggcg accaccctgc gtaaactgct gacgggtgag ctgctgaccc tggcaagccg
     1741 ccagcaactg attgattgga tggaagcgga taaagtggcg ggtccgctgc tgcgtagcgc
     1801 gctgccggct ggctggttta ttgcggataa aagcggtgcg ggcgaacgtg gcagccgtgg
     1861 cattattgcg gcgctgggcc cggatggtaa accgagccgt attgtggtga tttataccac
     1921 cggcagccag gcgacgatgg atgaacgtaa ccgtcagatt gcggaaattg gcgcgagcct
     1981 gattaaacat tggtaaaccg atacaattaa aggctccttt tggagccttt ttttttggac
     2041 gacccttgtc cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg
     2101 tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc
     2161 cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg
     2221 ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc
     2281 tgcgaggctg gccgtaggcc ggccccgtag aaaagatcaa aggatcttct tgagatcctt
     2341 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2401 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2461 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2521 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2581 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2641 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2701 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2761 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2821 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2881 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2941 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3001 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3061 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3121 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     3181 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaggcgcgc
     3241 ccagctgtct agggcggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
     3301 ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg cct
//
