LOCUS       sequence-248395-        2987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part O- ORF; arsR; transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP18-arsR_O
FEATURES             Location/Qualifiers
     source          1..2987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..60
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    457..477
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      511..605
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        678..769
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(770..838,839..1630)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1634..1673
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1820..1928
                     /note="incP origin of transfer"
     rep_origin      1964..2552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2453..2472
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2706..2723
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2840..2861
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(2895..2981)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttttgcag gtgcacctgc ttttcgctga attcgcggcc gcttctagag
       61 ggtctgcgat gtttggtctc tagcctcatt tctgttaccc atccaattgt tcaaaattct
      121 tgctgatgaa acccgtctgg gcatcgtttt actgctcagc gaactgggag agttatgcgt
      181 ctgcgatctc tgcactgctc tcgaccagtc gcagcccaag atctcccgcc acctggcatt
      241 gctgcgtgaa agcgggctat tgctggaccg caagcaaggt aagtgggttc attaccgctt
      301 atcaccgcat attccagcat gggcggcgaa aattattgat gaggcctggc gatgtgaaca
      361 ggaaaaggtt caggcgattg tccgcaacct ggctcgacaa aactgttccg gggacagtaa
      421 gaacatttgc agttcgtgag acctttcatc gcgacctact agtagcggcc gctgcaggga
      481 gttgtcttcg aagacttcgc tctagtcttg gactcctgtt gatagatcca gtaatgacct
      541 cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg
      601 agaatccagg ggtccccaat aattacgatt taaattagta gcccgcctaa tgagcgggct
      661 tttttttaat tcccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      721 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagcattca
      781 gcattttcgt gtggcgctga ttccgttttt tgcggcgttt tgcctgccgg tgtttgcgca
      841 tccggaaacc ctggtgaaag tgaaagatgc ggaagatcaa ctgggtgcgc gcgtgggcta
      901 tattgaactg gatctgaaca gcggcaaaat tctggaatct tttcgtccgg aagaacgttt
      961 tccgatgatg agcaccttta aagtgctgct gtgcggtgcg gttctgagcc gtgtggatgc
     1021 gggccaggaa caactgggcc gtcgtattca ttatagccag aacgatctgg tggaatatag
     1081 cccggtgacc gaaaaacatc tgaccgatgg catgaccgtg cgtgaactgt gcagcgcggc
     1141 gattaccatg agcgataaca ccgcggcgaa cctgctgctg acgaccattg gcggtccgaa
     1201 agaactgacc gcgtttctgc ataacatggg cgatcatgtg acccgtctgg atcgttggga
     1261 accggaactg aacgaagcga ttccgaacga tgaacgtgat accaccatgc cggcagcaat
     1321 ggcgaccacc ctgcgtaaac tgctgacggg tgagctgctg accctggcaa gccgccagca
     1381 actgattgat tggatggaag cggataaagt ggcgggtccg ctgctgcgta gcgcgctgcc
     1441 ggctggctgg tttattgcgg ataaaagcgg tgcgggcgaa cgtggcagcc gtggcattat
     1501 tgcggcgctg ggcccggatg gtaaaccgag ccgtattgtg gtgatttata ccaccggcag
     1561 ccaggcgacg atggatgaac gtaaccgtca gattgcggaa attggcgcga gcctgattaa
     1621 acattggtaa accgatacaa ttaaaggctc cttttggagc cttttttttt ggacgaccct
     1681 tgtccttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc
     1741 catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt
     1801 gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt
     1861 ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag
     1921 gctggccgta ggccggcccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1981 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2041 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2101 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2161 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2221 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2281 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2341 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2401 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2461 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2521 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2581 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     2641 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     2701 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     2761 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     2821 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaggc gcgcccagct
     2881 gtctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
     2941 cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcct
//
