LOCUS       sequence-248456-        3999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part T- Terminator;  L3S1P32; synthetic terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 13 to 47)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-L3S1P32_T
FEATURES             Location/Qualifiers
     source          1..3999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..47
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     misc_feature    101..122
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    232..252
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             280..300
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             320..1027
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     CDS             320..1027
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(344..372)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     967..987
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1067..1161
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1234..1325
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1326..1394,1395..2186)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2190..2229
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2376..2484
                     /note="incP origin of transfer"
     rep_origin      2520..3108
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3009..3028
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3262..3279
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3294..3434
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3360..3379)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3520..3542
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3536..3727)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3907..3993)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttgacggc tagctcagtc ctaggtacag tgctagcgta cttaacttct
       61 gactgagttg cacgcttaac ttctgactga gttgcacgct gaattcgcgg ccgcttctag
      121 agggtctgcg atgtttggtc tctgctttcg agacgaacaa taaggcctcc caaatcgggg
      181 ggccttttta tttttcaaca aaatgttcgc ttgagacctt tcatcgcgac ctactagtag
      241 cggccgctgc agggagtaac ttctgactga gttgcatact tgtttaactt taagaaggag
      301 atatacaatg gtagcccgta aaggagaaga acttttcact ggagttgtcc caattcttgt
      361 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      421 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      481 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
      541 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
      601 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
      661 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
      721 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
      781 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
      841 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
      901 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
      961 tcacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     1021 atacaaaggt tcgtaacttc tgactgagtt gcacgctcta gtcttggact cctgttgata
     1081 gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg
     1141 gcgtttttta ttggtgagaa tccaggggtc cccaataatt acgatttaaa ttagtagccc
     1201 gcctaatgag cgggcttttt tttaattccc ctatttgttt atttttctaa atacattcaa
     1261 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     1321 agagtatgag cattcagcat tttcgtgtgg cgctgattcc gttttttgcg gcgttttgcc
     1381 tgccggtgtt tgcgcatccg gaaaccctgg tgaaagtgaa agatgcggaa gatcaactgg
     1441 gtgcgcgcgt gggctatatt gaactggatc tgaacagcgg caaaattctg gaatcttttc
     1501 gtccggaaga acgttttccg atgatgagca cctttaaagt gctgctgtgc ggtgcggttc
     1561 tgagccgtgt ggatgcgggc caggaacaac tgggccgtcg tattcattat agccagaacg
     1621 atctggtgga atatagcccg gtgaccgaaa aacatctgac cgatggcatg accgtgcgtg
     1681 aactgtgcag cgcggcgatt accatgagcg ataacaccgc ggcgaacctg ctgctgacga
     1741 ccattggcgg tccgaaagaa ctgaccgcgt ttctgcataa catgggcgat catgtgaccc
     1801 gtctggatcg ttgggaaccg gaactgaacg aagcgattcc gaacgatgaa cgtgatacca
     1861 ccatgccggc agcaatggcg accaccctgc gtaaactgct gacgggtgag ctgctgaccc
     1921 tggcaagccg ccagcaactg attgattgga tggaagcgga taaagtggcg ggtccgctgc
     1981 tgcgtagcgc gctgccggct ggctggttta ttgcggataa aagcggtgcg ggcgaacgtg
     2041 gcagccgtgg cattattgcg gcgctgggcc cggatggtaa accgagccgt attgtggtga
     2101 tttataccac cggcagccag gcgacgatgg atgaacgtaa ccgtcagatt gcggaaattg
     2161 gcgcgagcct gattaaacat tggtaaaccg atacaattaa aggctccttt tggagccttt
     2221 ttttttggac gacccttgtc cttttccgct gcataaccct gcttcggggt cattatagcg
     2281 attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt
     2341 gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca
     2401 cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg
     2461 aatcctgctc tgcgaggctg gccgtaggcc ggccccgtag aaaagatcaa aggatcttct
     2521 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2581 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2641 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     2701 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2761 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2821 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2881 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2941 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3001 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3061 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3121 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3181 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3241 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     3301 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     3361 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     3421 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     3481 tgctcccggc atccgcttac agacaagctg tgacagtctc cgggagctgc atgtgtcaga
     3541 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     3601 cgtgcagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     3661 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     3721 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     3781 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     3841 gttgtgaggg taaacaactg gcggtatgga tgcggcgggg gcgcgcccag ctgtctaggg
     3901 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     3961 ccagtctttc gactgagcct ttcgttttat ttgatgcct
//
