LOCUS       sequence-24858-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Bissonnette SA, Glazier CM, Stewart MQ, Brown GE, Ellson CD, Yaffe 
            MB
  TITLE     Phosphatidylinositol 3-phosphate-dependent and -independent 
            functions of p40phox in activation of the neutrophil NADPH oxidase.
  JOURNAL   J Biol Chem. 2008 Jan 25. 283(4):2108-19.
  PUBMED    18029359
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2008 Jan 25. 283(4):2108-19."
COMMENT     Sequence Label: p40 E259A
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             65..82
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             92..109
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             113..145
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 tgnggaanat tccntctana atattttgtt tactttaaga aggagatata ccatgggcag
       61 cagccatcat catcatcatc acagcagcgg cctggtgccg cgcggcagcc atatggctag
      121 catgactggt ggacagcaaa tgggtcgcgg atccgaattc atggctgtgg cccagcagct
      181 gcgggccgag agtgactttg aacagcttcc ggatgatgtt gccatctcgg ccaacattgc
      241 tgacatcgag gagaagagag gcttcaccag ccactttgtt ttcgtcatcg aggtgaagac
      301 aaaaggagga tccaagtacc tcatctaccg ccgctaccgc cagttccatg ctttgcagag
      361 caagctggag gagcgcttcg ggccagacag caagagcagt gccctggcct gtaccctgcc
      421 cacactccca gccaaagtct acgtgggtgt gaaacaggag atcgccgaga tgcggatacc
      481 tgccctcaac gcctacatga agagcctgct cagcctgccg gtctgggtgc tgatggatga
      541 ggacgtccgg atcttctttt accagtcgcc ctatgactca gagcaggtgc cccaggcact
      601 ccgccggctc cgcccgcgca cccggaaagt caagagcgtg tccccacagg gcaacagcgt
      661 ggaccgcatg gcagctccga gagcagaggc tctatttgac ttcntggaaa acagcaactt
      721 cganctgant tcaagctgga gatgtgatct tcctcctcag tcgatcaaca agatggctgn
      781 aggcntgtcg ggagcacggc tcttccttct cttctgagnc tca
//
