LOCUS       sequence-248585-        2053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ConL3 and human D1 insulator as a Type 1 part to be used in 
            the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK1_025
FEATURES             Location/Qualifiers
     source          1..2053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     423..442
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1041..1075)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1146..1805)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1720..1739
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1987..2053,1..522)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
       61 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
      121 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
      181 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      241 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      301 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      361 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      421 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      481 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      541 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      601 cctgattctg tggataaccg tagtcggtct caccctgaat tcgcatctag agcagaccta
      661 gaccacatga ggctgatgta ggacagccac cagtggcagc taatcaacac cgcaagatgc
      721 cgatgcacgc tcatatcatc gtcgtcagcc tggtagccat tcgacatacg gatcagggaa
      781 ctcgaaccca gtaccgtctc agttctgtat gggcacagac aacctaacta atttcctggc
      841 cctcaaggtg acttgcttga acttgccacc ttcgccacta gggggcagca ttggtttaca
      901 cagggtgaac aaacgttcaa ttctaaacta atattctttg gtgggaaagt gtgttcattt
      961 tggtttgttt tgaagaagtc acttcatacc tttgaactgg ggttaagctg gttaactccc
     1021 aaaaacgtga gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     1081 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     1141 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     1201 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     1261 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     1321 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac
     1381 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     1441 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     1501 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     1561 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
     1621 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     1681 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     1741 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     1801 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     1861 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa
     1921 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     1981 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2041 gctaccagcg gtg
//
