LOCUS       sequence-248664-        2928 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Gs-coupled rM3D as a Type 3 part to be used in the MTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3_016
FEATURES             Location/Qualifiers
     source          1..2928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     500..519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             714..740
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      complement(1993..2027)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2098..2757)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2672..2691
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
       61 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      661 tctttcctgc gttatcccct gattctgtgg ataaccgtag tcggtctcat atgtacccat
      721 acgatgttcc cgactacgcc accttgcaca gtaacagtac aacctcgcct ttgtttccca
      781 acatcagctc ttcctgggtg cacagtccct cggaggcagg gctgcccttg gggacagtca
      841 ctcagttggg cagctacaac atttcacaag aaactgggaa tttctcctca aacgacacct
      901 ccagcgaccc tctcgggggt cacaccatct ggcaagtggt cttcattgcc ttcttaactg
      961 gcttcctggc attggtgacc atcattggca acatccttgt cattgtggcc ttcaaggtca
     1021 acaaacagct gaagacagtc aacaactact tcctcttaag cctggcctgt gcagacctga
     1081 tcatcggggt catttccatg aacctgttca ctacctacat cattatgaac cgttgggcac
     1141 tggggaactt agcctgcgac ctctggctct ccattgactg tgtggccagc aatgcctctg
     1201 tcatgaatct gctggtcatc agctttgaca ggtacttttc catcacttct ccattccgct
     1261 accagagcct gatgaccagg gctcgagctg gtgtgatgat tggtctggct tgggtcatct
     1321 cctttgtcct atgggctcct gccatcttgt tctggcaata ctttgtaggg aagagaactg
     1381 tgcccccagg agaatgtttc attcagtttc tgagtgagcc caccatcacc ttcggcacgg
     1441 cgatcgctgg cttttacatg cctgtcacca tcatgactat tttatactgg cgggtgtacc
     1501 gggaggccaa ggagcagatc aggaagatcg accgctgcga gggccggttc tatggcagcc
     1561 aggagcagcc gcagccaccc ccgctccccc aacaccagcc catcctcggc aacggccgtg
     1621 ccagcaagag gaagacgtcc cgtgtcatgg ccatgaggga acacaaagct ctgcagacgc
     1681 tcagtgccat cttgctagcc ttcatcatca cgtggacccc ctacaacatc atggtcctgg
     1741 tgaacacctt ctgtgacagc tgcataccca aaacctattg gaatctgggc tactggctgt
     1801 gctatatcaa cagcaccgtg aaccctgtgt gctatgccct gtgcaacaaa acattcagaa
     1861 ccaccttcaa gacgctcctc ttgtgccagt gtgacaaaag gaagaggcgc aaacagcagt
     1921 accagcagag acagtcggtc atttttcaca agcgagtgcc ggagcaggcc ttgggatcct
     1981 gagaccagac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     2041 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
     2101 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     2161 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     2221 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     2281 gtttaaatca aaactggtga aactcaccca gggattggct gaaacgaaaa acatattctc
     2341 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     2401 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     2461 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     2521 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
     2581 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     2641 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     2701 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     2761 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     2821 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aatcatgacc aaaatccctt
     2881 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatca
//
