LOCUS       sequence-248691-        2571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes p38 Translocation Reporter (p38 KTR) fused to mCerulean3 as 
            a Type 3 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3_031
FEATURES             Location/Qualifiers
     source          1..2571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     366..385
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(766..768,769..771,772..1482)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(811..832)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1072..1091)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1419..1440
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      complement(1502..1536)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1607..2266)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2181..2200
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2448..2571,1..465)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
       61 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      121 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      181 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      241 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      301 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      361 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      421 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      481 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      541 tcccctgatt ctgtggataa ccgtagtcgg tctcatatgc gtaagccaga tctccgcgtt
      601 cttatcccac ctggcagcaa gaacacgatg ccatcagtga atcaaaggat aaataactcc
      661 aagcgctcgg ccggactcaa cgaagacgag tcgccatcta agaagctggc gtcgccggag
      721 gtgtcgtcaa ggctggagcg cctgaccctc cagtccagca ctagtatggt gagcaagggc
      781 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      841 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      901 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      961 agctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     1021 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1081 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1141 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1201 gccatccacg gcaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
     1261 ttcggcctga actgcaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1321 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     1381 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1441 accgccgccg ggatcactct cggcatggac gagctgtaca agggatcctg agaccagacc
     1501 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     1561 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
     1621 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     1681 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     1741 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     1801 aactggtgaa actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt
     1861 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     1921 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     1981 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     2041 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     2101 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     2161 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     2221 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     2281 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     2341 gaaagttgga acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt
     2401 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2461 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2521 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag c
//
