LOCUS       sequence-248695-        4293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Gibberellin Receptor fused to VPR activation domain as a 
            Type 3 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3_033
FEATURES             Location/Qualifiers
     source          1..4293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     366..385
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1639..1788
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             1795..1815
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2635..3204
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     terminator      complement(3224..3258)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(3329..3988)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3903..3922
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(4170..4293,1..465)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
       61 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      121 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      181 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      241 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      301 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      361 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      421 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      481 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      541 tcccctgatt ctgtggataa ccgtagtcgg tctcatatgg ctgcgagcga tgaagttaat
      601 cttattgaga gcagaacagt ggttcctctc aatacatggg ttttaatatc caacttcaaa
      661 gtagcctaca atatccttcg tcgccctgat ggaaccttta accgacactt agctgagtat
      721 ctagaccgta aagtcactgc aaacgccaat ccggttgatg gggttttctc gttcgatgtc
      781 ttgattgatc gcaggatcaa tcttctaagc agagtctata gaccagctta tgcagatcaa
      841 gagcaacctc ctagtatttt agatctcgag aagcctgttg atggcgacat tgtccctgtt
      901 atattgttct tccatggagg tagctttgct cattcttctg caaacagtgc catctacgat
      961 actctttgtc gcaggcttgt tggtttgtgc aagtgtgttg ttgtctctgt gaattatcgg
     1021 cgtgcaccag agaatccata cccttgtgct tatgatgatg gttggattgc tcttaattgg
     1081 gttaactcga gatcttggct taaatccaag aaagactcaa aggtccatat tttcttggct
     1141 ggtgatagct ctggaggtaa catcgcgcat aatgtggctt taagagcggg tgaatcggga
     1201 atcgatgttt tggggaacat tctgctgaat cctatgtttg gtgggaatga gagaacggag
     1261 tctgagaaaa gtttggatgg gaaatacttt gtgacggtta gagatcgcga ttggtactgg
     1321 aaagcgtttt tacccgaggg agaagataga gagcatccag cgtgtaatcc gtttagcccg
     1381 agagggaaaa gcttagaagg agtgagtttc cccaagagtc ttgtggttgt cgcgggtttg
     1441 gatttgatta gagattggca gttggcatac gcggaagggc tcaagaaagc gggtcaagag
     1501 gttaagctta tgcatttaga gaaagcaact gttgggtttt acctcttgcc taataacaat
     1561 catttccata atgttatgga tgagatttcg gcgtttgtaa acgcggaatg tgaattctca
     1621 agcgctgcag gtactagtga tgctttagac gattttgact tagatatgct tggttcagac
     1681 gcgttagacg acttcgacct agacatgtta ggctcagatg cattggacga cttcgattta
     1741 gatatgttgg gctccgatgc cctagatgac tttgatctag atatgctagg tagtcccaaa
     1801 aagaagagga aagtgggatc ccagtatctg cccgacacag atgatagaca ccgaatcgaa
     1861 gagaaacgca agcgaacgta tgaaaccttc aaatcgatca tgaagaaatc gcccttctcg
     1921 ggtccgaccg atcccaggcc cccaccgaga aggattgcgg tcccgtcccg ctcgtcggcc
     1981 agcgtgccga agcctgcgcc gcagccctac cccttcacgt cgagcctgag cacaatcaat
     2041 tatgacgagt tcccgacgat ggtgttcccc tcgggacaaa tctcacaagc ctcggcgctc
     2101 gcaccagcgc ctccccaagt ccttccgcaa gcgcctgccc cagcgcctgc accggcaatg
     2161 gtgtccgccc tcgcacaggc ccctgcgccc gtccccgtgc tcgcgcctgg accgccccag
     2221 gcggtcgctc caccggctcc gaagccgacg caggccggag agggaacact ctccgaagca
     2281 cttcttcaac tccagtttga tgacgaggat cttggagcac tccttggaaa ctcgacagac
     2341 cctgcggtgt ttaccgacct cgcgtcagta gataactccg aatttcagca gcttttgaac
     2401 cagggtatcc cggtcgcgcc acatacaacg gagcccatgt tgatggaata ccccgaagca
     2461 atcacgagac ttgtgacggg agcgcagcgg cctcccgatc ccgcacccgc acctttgggg
     2521 gcacctggcc tccctaacgg acttttgagc ggcgacgagg atttctcctc catcgccgat
     2581 atggatttct cagtcttgct gtcacagatt tccagcggct ctggcagcgg cagccgggat
     2641 tccagggaag ggatgttttt gccgaagcct gaggccggct ccgctattag tgacgtgttt
     2701 gagggccgcg aggtgtgcca gccaaaacga atccggccat ttcatcctcc aggaagtcca
     2761 tgggccaacc gcccactccc cgccagcctc gcaccaacac caaccggtcc agtacatgag
     2821 ccagtcgggt cactgacccc ggcaccagtc cctcagccac tggatccagc gcccgcagtg
     2881 actcccgagg ccagtcacct gttggaggat cccgatgaag aaacgagcca ggctgtcaaa
     2941 gcccttcggg agatggccga tactgtgatt ccccagaagg aagaggctgc aatctgtggc
     3001 caaatggacc tttcccatcc gcccccaagg ggccatctgg atgagctgac aaccacactt
     3061 gagtccatga ccgaggatct gaacctggac tcacccctga ccccggaatt gaacgagatt
     3121 ctggatacct tcctgaacga cgagtgcctc ttgcatgcca tgcatatcag cacaggactg
     3181 tccatcttcg acacatctct gtttggatcc tgagaccaga ccaataaaaa acgcccggcg
     3241 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa
     3301 caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact
     3361 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
     3421 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     3481 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     3541 agggattggc tgaaacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     3601 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     3661 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     3721 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga aattccggat
     3781 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     3841 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     3901 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     3961 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     4021 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     4081 cgtgcccgat caatcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4141 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4201 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4261 ccaactcttt ttccgaaggt aactggcttc agc
//
