LOCUS       sequence-248749-       11121 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GAL4-induced expression of codon-optimised FLP recombinase with 
            unfused mNeongreen (mNG) as a marker in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11121)
  AUTHORS   Ayuso C, Askjaer P
  TITLE     Spatiotemporal control of genome recombination through combined 
            FLP-Frt and GAL4-UAS technologies.
  JOURNAL   MicroPubl Biol. 2019 Jan 29;2019:10.17912/micropub.biology.000089. 
            doi: 10.17912/micropub.biology.000089.
  PUBMED    32550473
REFERENCE   2  (bases 1 to 11121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2019-01-29"; volume: 
            "2019:10.17912/micropub.biology.000089. doi"; pages: " 
            10.17912/micropub.biology.000089"
COMMENT     Sequence Label: pBN449
FEATURES             Location/Qualifiers
     source          1..11121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(43..631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(123..142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(802..1593,1594..1662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1425..1444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1663..1767)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1835..1853
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1891..1913)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2013..2032
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2226..2248
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2240..2257
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2241..2257
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2265..2285
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3333..3357
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    3471..3565
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3604..3698
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3737..3831
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             9318..9329
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(9478..9502)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(11002..11018)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11002..11018)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11015..11037)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 gcaaaagacc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
       61 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      481 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
      721 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
      781 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
      841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
      901 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
      961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     1681 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1741 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     1801 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     1861 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     1921 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     1981 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     2041 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     2101 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     2161 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     2221 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttatcaacta tgtataataa
     2281 agttgcggcc cattaggtca gacaaaaatt tctgatcttt aaaccttatt gattaaaact
     2341 aatttttgcc cgactttttt gacacatgca ctagacgctt gtactcaaaa tttggaaacc
     2401 ttgctcaaat ccactactca gatatgacag tagcatcgat aattgaacca ttatcctgaa
     2461 atcccctttc ctgtcccggt tcattttcca attttttcaa cggtggaaga aggggcaaat
     2521 tttataccaa aacgtctccg tttatgaccc accccctatg caaaataaac ggctctggga
     2581 gaaatagtac agaaaacgcg gagcctgctt gattccttct ccgaggcgac aaaaaacgca
     2641 tcattcgtat tgtaagatgc atgtgtggct ggcctctttt gaattttact ggttttttat
     2701 atttctacta atatttcgaa aaattttgta agtaaatcat tgattacgtt tacaaaaatt
     2761 tagaaacatt tcaaaatgat tacttctaaa ttaattatat ttacaggtta gttcgagtta
     2821 cttatgacct tccaatccgc catatccgcc aaggacgctc caattaattt tttagaatta
     2881 ttttttaaca catgtattat tttttaacaa taaattattc atgaaatcat caaatgtata
     2941 tgttctacta taatatttag gtcaaactat agaaaagaaa tccttgtttt tggaacaaag
     3001 gagttcagat cctgtgcatt tgatcttgga tccacatctt tggtccatgg gaaagcttgg
     3061 cactgatcaa gaaggattct tcaaatatct aaaattgccg gctgcccgta gaattccatc
     3121 agttttccag agttttagtg aaaatttgca tcccgttaga aacaatctac acaacggtaa
     3181 aaaggaccca ttatttttac cgtaccgttt tcctttatgg ccctctatgc aaaaaattat
     3241 agcgtggagg ggcagtacag aataataaag tgtgagaaaa aaaactgctc tgcgagaaat
     3301 agtacagcaa acgcggagca tgcttgattc tatccacttt gtacaagaaa gctgggttaa
     3361 tacgactcac tagtgggcag atcttcgaat gcatcgcgcg caccgtacgt ctcgaggagc
     3421 cggctgtagc atgcggccct tgctagccca tgattacgcc aagctacggg cggagtactg
     3481 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
     3541 tccgagcgga gtactgtcct ccgagcggag actccctagc ccatgattac gccaagctac
     3601 gggcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag
     3661 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagactccct agcccatgat
     3721 tacgccaagc tacgggcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
     3781 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagactc
     3841 cctaggggcg gccgcactga ctgggccggc caaaaatgca taaggttttg ctggcactgt
     3901 tctttatctt tctggcacca gcatccgcac tggcagtctc cgaaccggcc tgcaggtcga
     3961 ttttttgcaa attacgagcg ttgtaggggg cggagcgata ggtcctatag gttttggtat
     4021 atcatcattc attcattcat tggtacattc atttacccac cttcctcttt ctgagcttct
     4081 ctggagttct gtgcttcctt tttcccttat ctttatactg taatttttaa ctttcaggca
     4141 ttgattggcg cgcctctaga ggatccccgg gattggccaa aggacccaaa ggtatgtttc
     4201 gaatgatact aacataacat agaacatttt caggaggacc cttggctagc gtcgacggta
     4261 ccaaaaatgc cacaattcga cattctctgt aagacaccac caaaagtcct cgtccgtcaa
     4321 ttcgtcgagc gtttcgagcg tccatccgga gagaagatcg ccctctgcgc cgccgagctc
     4381 acctacctct gctggatgat cacccacaac ggaaccgcca tcaagcgtgc caccttcatg
     4441 tcctacaaca ccatcatctc caactccctc tccttcgaca tcgtcaacaa gtccctccaa
     4501 ttcaagtaca agacccaaaa ggccaccatc ctcgaggcct ccctcaaggt aagtttaaac
     4561 atatatatac taactaaccc tgattattta aattttcaga agctcatccc agcctgggag
     4621 ttcaccatca tcccatacta cggacaaaag caccaatccg acatcaccga catcgtctcc
     4681 tccctccaac tccaattcga gtcctccgag gaggccgaca agggaaactc ccactccaag
     4741 aagatgctca aggccctcct ctccgaggga gagtccatct gggagatcac cgagaagatc
     4801 ctcaactcct tcgagtacac ctcccgtttc accaagacca agaccctcta ccaattcctc
     4861 ttcctcgcca ccttcatcaa ctgcggacgt ttctccgaca tcaagaacgt cgacccaaag
     4921 tccttcaagc tcgtccaaaa caagtacctc ggagtcatca tccaatgcct cgtcaccgag
     4981 accaagacct ccgtctcccg tcacatctac ttcttctccg cccgtggacg tatcgaccca
     5041 ctcgtctacc tcgacgagtt cctccgtaac tccgagccag tcctcaagcg tgtcaaccgt
     5101 accggaaact cctcctccaa caagcaagag taccaactcc tcaaggacaa cctcgtccgt
     5161 tcctacaaca aggccctcaa gaagaacgcc ccatactcca tcttcgccat caagaacgga
     5221 ccaaagtccc acatcggacg tcacctcatg acctccttcc tctccatgaa ggtaagttta
     5281 aacatgattt tactaactaa ctaatctgat ttaaattttc agggactcac cgagctcacc
     5341 aacgtcgtcg gaaactggtc cgacaagcgt gcctccgccg tcgcccgtac cacctacacc
     5401 caccaaatca ccgccatccc agaccactac ttcgccctcg tctcccgtta ctacgcctac
     5461 gacccaatct ccaaggagat gatcgccctc aaggacgaga ccaacccaat cgaggagtgg
     5521 caacacatcg agcaactcaa gggatccgcc gagggatcca tccgttaccc agcctggaac
     5581 ggaatcatct cccaagaggt cctcgactac ctctcctcct acatcaaccg tcgtatctaa
     5641 cgcgtgctgt ctcatcctac tttcacctag ttaactgctt gtcttaaaat ctatgcttct
     5701 ctttagtatc taaaattttc ctagaagctt acaagtatat aaatggtctc ttctcaataa
     5761 aggttgtata tttattcatc ttattgaatc tgccatttcc tcgtttttgc gagtttatat
     5821 accttccaat tttctttcta ttgtattttc aacttctaat tttaattcag ggaaactgct
     5881 tcaacgcatc atggtgtcga agggagaaga ggataacatg gcttcactcc cagctacaca
     5941 cgaactccac atcttcggat cgatcaacgg agtggatttc gatatggtcg gacaaggtaa
     6001 gtttaaacat atatatacta actaaccctg attatttaaa ttttcaggaa ctggaaaccc
     6061 aaacgatgga tacgaggaac tcaacctcaa gtcgacaaag ggagatctgc aattctcgcc
     6121 atggattctc gtgccacaca tcggatacgg attccaccaa tacctcccat acccaggtaa
     6181 gtttaaactg agttctacta actaacgagt aatatttaaa ttttcagatg gaatgtcacc
     6241 attccaagct gccatggtgg atggatcggg ataccaagtt caccgaacaa tgcaattcga
     6301 ggatggagcc tcgctcacag tgaactaccg atacacatac gagggatcgc acatcaaggt
     6361 aagtttaaac agttcggtac taactaacca tacatattta aattttcagg gagaggctca
     6421 agttaaggga acaggattcc cagctgatgg accagtgatg acaaactcac tcacagctgc
     6481 tgattggtgc cgatcgaaaa agacataccc aaatgataag acaatcatct cgacattcaa
     6541 gtggtcgtac actactggaa acggaaagcg ataccgatcg acagcccgaa caacatacac
     6601 attcgctaag ccaatggccg ccaactacct caaggtaagt ttaaacatga ttttactaac
     6661 taactaatct gatttaaatt ttcagaatca accaatgtac gtgttccgaa agacagaact
     6721 caagcactca aagacagagc tgaacttcaa agagtggcaa aaggccttca cagatgtgat
     6781 gggaatggat gaactctaca agtaacgcgt atgtatctgc atatccttca aatgttgtcc
     6841 atattgtatc agtaaattat aatgccccct ttttatatat tctccttatc atgtaaatgt
     6901 tctattgatt ttttgctggt gtaaaacgtt tttatagtaa atcctctcca cagtgaattt
     6961 gttaaattgt atcaaaacaa gacattgatt aaatttgttc gagacttgtg gattgtaaaa
     7021 tattttgcag aaaatggcaa tattcattta gaaacattac ttccataata ggaaaaacta
     7081 tttatgagtt ttcatgtgcc tatgcaagtt cccactctgt gtgaatttca tcatacaaat
     7141 tgagcaaaca taaggtttat gtccagtatg ggttctcaga tgttttgtaa gagttgagct
     7201 gtcggtgaat gccttctcac attgagcaca tggatacggt ctctctccag tgtgtgttct
     7261 gaaataatga aaaatagtct tgcaatatgt ttctactatt cgagaaaaaa tgtaattttt
     7321 tttcttatgg taacccggct ttccccgggc ctagttctag acattctcta atgaaaaaat
     7381 ctttcagttg aaattgaaaa tgagttaaag ttggagtttt tattgaaaac agatttccgt
     7441 gtgattagtg tttttagcga gtgtgacagg acagcgaaaa aatatagaaa caagggggga
     7501 actgaaaagc ttaggaatgc attgaacatg agaaggggaa ggggaaggaa caaactagac
     7561 aggaattatt ggaatttaat cacatttgga gttttttttc tattcgacag aataattatc
     7621 cagaacattt ttgtattaaa tatttatgca tcatatgagt agtcggcttt gttgtgcatg
     7681 acgagtttgt tatcgacgaa atagaagctg tcagaacgag tctcgtttgg attgttgatc
     7741 atgtcgtcca ctgaaaaaga gattagtctt ttgaattgta ctttttagaa taatgactca
     7801 ctgagttgtt gagagagttg agggaactca tagatatgtt cacagttgtt tcgtgaattc
     7861 ggaatacaga atccgaattc aaagtcaaaa cacttcagaa ggcgatcttt gaagaagtga
     7921 cgttcgatca ttcggaaatg atggattggg atgtctccta ctttgaattc cacagttgct
     7981 ccgaccgttt tgagtttcaa aaagtttgga gcaaatctat aacgcacgta tcttgccgac
     8041 tcctgtggcg actcatcatt ctcctgatcg ttctccggtt tggcgatctc gaaaagcact
     8101 tgctcagtgt ccagatcacg gatttggaac ttggtgaact cgatgttata gatgttcgca
     8161 gatggggagc ataagaatcc taaatttatg ttttaaactg aaatccaaag ggagcaagat
     8221 accttgagtg attcccggaa gtgctaaaac gtcgttcgga gtgatttgag ctttcttcgc
     8281 aagctccgat tccgttgtga ttccttgttc ggtgcttggt ggtggccgtg gcatctggaa
     8341 atatggaaaa gttcaacaaa aagaaaagag aaaagaatga aatcggatat caagagttag
     8401 ttgagcggtt tctctagttt tctgagtctc acctgcgacg ggaaggtcgc cgagccgggt
     8461 ggaatcgatc gttgttgctc ggctttcata tcggtttggt tggaagcggc tgaaaacgga
     8521 aagaagtgga agaaggaaaa gagtgtggtg tgacaggaaa atggtaatta gagggtgcca
     8581 aataaccagc tatattttgt ttttttttga aaacattttt aaaaagaaaa atacgataat
     8641 gatatcagat ggatttccgg aaaactggta tgaaaaattt caaccttttt gagtacatgt
     8701 aatcaaaata cactttgtaa attatcattt ttattgaaac tccaccattt ttctatttat
     8761 aacgctaata atttgaaaaa gaaacctatt gcgaaccgcg gggtgaatcc caaaaacgaa
     8821 tgcgttttgg tggagtgatt gattcgaatc gaagaagaaa aagaagaaga cgtggaatag
     8881 agagctcact cttaaccgag cagcacacac cgacagaaaa aaaaatgaaa tgaatgaggg
     8941 tcttcttctt cttcttcttc gaatgattga cagaaatggg aaaaagagga agattgagaa
     9001 gggaaaaagg aaggagaaaa gaagcagaag aagacgtcag agaggagagg aacgagcgga
     9061 aaagcagcgg gcgcaagtca tagaagtagc agagctgggg agaagaagac actatccaag
     9121 aaaggaatga cgagagagta tgcaaagggg tatagggtgc agacagaata ggaacagaat
     9181 aacagatgat gagccaagaa gagttgaaaa gggcgatgaa tttgtcatgt aacttaattt
     9241 gggtcaattt gagcatgatg aattgaaatc atcccttgtt gggagttaat aaccggtttg
     9301 ttatcagaaa ccctgtaata gaagggcgcc ctaactttga gccaattcat cccggtttct
     9361 gtcaaatata tcaaaaagtg gtcaactgac aaattgtttt tgatattata ataaacattt
     9421 tatccgttaa caattttcga atacttttta caaggacttg gataaattgg ctcaaagagc
     9481 ctgctttttt gtacaaactt gaattcaaaa acgcagaact cgtactgttg gatgcctgtg
     9541 tagcggtcct ctattgattt ttgctatttt ttgataattt tactgtaaat tatgagttat
     9601 tccaattaac attcctagct aaatgtaagt tagcgaccaa ttttttagca accccatttt
     9661 atgacttttc agaatatcgc ctactgggtc aacgacactt gtttggaact aagggtcttc
     9721 ccaatttttt ttaaatcttc ataggcttga cagaatcagg gttcttcaag cagctttgag
     9781 agggaatcaa ttgcaacatt tgatgttaaa tttatatttt ttatagttct ctggtgaaat
     9841 ggggaccacc gcaaagcccc ttttatgttt ttaacttgaa taaaatcttg tattaaattg
     9901 tgatttatag atctgtatgg gtaatttgaa ggaaaagaaa gggagctccc aaaagtgcct
     9961 tacagatcca ggcccaataa atgtaaacta ttgggcagta gtatggcaaa aatcgtgcgg
    10021 aatttgaaaa acctagaatt aaaataaata ttcaacacaa atgttcgaga tattttaagt
    10081 tcggagtgta tatctagaac atattgtaca agtaggtcta cgggatcgtc tacgggaaaa
    10141 tttgatttcc tcgaagatgt atttaatgaa ttctctaatg ttgcaggtct ttgtatttga
    10201 cagaatgttg gtttttgaaa cttattcgcc ataattgctt ggctactaca tgtctcatgc
    10261 cattgacact atcctgtact cgaaatttgt taagtaacta cccaccgtcc acttatgacg
    10321 agagggtcaa ttaaggtcac tccacaaaag taaatttgaa aacacatgag aaaaagtgat
    10381 ctgaattccc ttgaagtggc taaaacaaag cagatgaggg atacgacgta tgcgactact
    10441 gagagataag gtcgaaactt gataaggatg ctgcttacct gcttagcaag tttcttcgca
    10501 caggagaaag tgacaagcta aactatcaaa ttcatctaaa acctcaaaaa attattttgg
    10561 atgaaataat gttttatttt gagcaacgca taaggcaaag aataaattaa aggcgatccg
    10621 cttagcggcg agttccgtcc tcgaagaaga ttcctccatc gatggcgcag tactttggac
    10681 agattccacg ctctcctggg gttcctggtg gtccgactgg tcctggctgt cctgggtttc
    10741 cgtcggctcc tggcttgccg tctggtcctg gtggtccctt tggtccaggt tgtcctgaaa
    10801 gatgtggtga ttagatatgg attgaaaaac tatgaaatga ttacctgggg ttccgtcgtt
    10861 tcctggttgt ccggatggtc ctggtggtcc gactggtcct ggctctccgg tggatcctgg
    10921 ctctcccttt tggactggct cgcagacagc tggaagtccc tccaactttc tatacaaagt
    10981 tgatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctggt
    11041 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    11101 gaacatgtga gcaaaaggcc a
//
