LOCUS       sequence-248754-        8303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina 
            library preperation. Developed for the large scale ChIP-Seq and 
            ATAC-Seq experiments for the fruitENCODE and Maize Cistrome 
            projects..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Tu X, Mejia-Guerra MK, Valdes Franco JA, Tzeng D, Chu PY, Shen W, 
            Wei Y, Dai X, Li P, Buckler ES, Zhong S
  TITLE     Reconstructing the maize leaf regulatory network using ChIP-seq data
            of 104 transcription factors.
  JOURNAL   Nat Commun. 2020 Oct 9;11(1):5089. doi: 10.1038/s41467-020-18832-8.
  PUBMED    33037196
REFERENCE   2  (bases 1 to 8303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-10-9"; volume: "11(1):5089. doi"; pages: " 
            10.1038/s41467-020-18832-8"
COMMENT     Sequence Label: pET30a-HS-Tn5
FEATURES             Location/Qualifiers
     source          1..8303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             938..2362
                     /codon_start=1
                     /product="transposase from the bacterial Tn5 transposon 
                     (Reznikoff, 1993)"
                     /label=Tn5 transposase
                     /translation="ITSALHRAADWAKSVFSSAALGDPRRTARLVNVAAQLAKYSGKSI
                     TISSEGSKAMQEGAYRFIRNPNVSAEAIRKAGAMQTVKLAQEFPELLAIEDTTSLSYRH
                     QVAEELGKLGSIQDKSRGWWVHSVLLLEATTFRTVGLLHQEWWMRPDDPADADEKESGK
                     WLAAAATSRLRMGSMMSNVIAVCDREADIHAYLQDKLAHNERFVVRSKHPRKDVESGLY
                     LYDHLKNQPELGGYQISIPQKGVVDKRGKRKNRPARKASLSLRSGRITLKQGNITLNAV
                     LAEEINPPKGETPLKWLLLTSEPVESLAQALRVIDIYTHRWRIEEFHKAWKTGAGAERQ
                     RMEEPDNLERMVSILSFVAVRLLQLRESFTPPQALRAQGLLKEAEHVESQSAETVLTPD
                     ECQLLGYLDKGKRKRKEKAGSLQWAYMAIARLGGFMDSKRTGIASWGALWEGWEALQSK
                     LDGFLAAKDLMAQGIKI"
     CDS             2363..2956
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     CDS             2987..3142
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     CDS             3164..3181
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3234..3252)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3248..3295
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3332..3787
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3419..3438)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3629..3650
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3879..4694)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3879..4694)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4602..4621
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4686..4705
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4816..5404
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5305..5324
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5558..5575
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5590..5729
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5655..5674)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5815..5837
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5831..6022)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6831..7913)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7875..7894
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7914..7991)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     8197..8216
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atatacatat ggctgaaatt accgcatccc tggtaaaaga
      121 gctgcgtgag cgtactggcg caggcatgat ggattgcaaa aaagcactga ctgaagctaa
      181 cggcgacatc gagctggcaa tcgaaaacat gcgtaagtcc ggtgctatta aagcagcgaa
      241 aaaagcaggc aacgttgctg ctgacggcgt gatcaaaacc aaaatcgacg gcaactacgg
      301 catcattctg gaagttaact gccagactga cttcgttgca aaagacgctg gtttccaggc
      361 gttcgcagac aaagttctgg acgcagctgt tgctggcaaa atcactgacg ttgaagttct
      421 gaaagcacag ttcgaagaag aacgtgttgc gctggtagcg aaaattggtg aaaacatcaa
      481 cattcgccgc gttgctgcgc tggaaggcga cgttctgggt tcttatcagc acggtgcgcg
      541 tatcggcgtt ctggttgctg ctaaaggcgc tgacgaagag ctggttaaac acatcgctat
      601 gcacgttgct gcaagcaagc cagaattcat caaaccggaa gacgtatccg ctgaagtggt
      661 agaaaaagaa taccaggtac agctggatat cgcgatgcag tctggtaagc cgaaagaaat
      721 cgcagagaaa atggttgaag gccgcatgaa gaaattcacc ggcgaagttt ctctgaccgg
      781 tcagccgttc gttatggaac caagcaaaac tgttggtcag ctgctgaaag agcataacgc
      841 tgaagtgact ggcttcatcc gcttcgaagt gggtgaaggc atcgagaaag ttgagactga
      901 ctttgcagca gaagttgctg cgatgtccaa gcagtctatt accagtgcac tgcatcgtgc
      961 ggcggattgg gcgaaaagcg tgttttctag tgctgcgctg ggtgatccgc gtcgtaccgc
     1021 gcgtctggtg aatgttgcgg cgcaactggc caaatatagc ggcaaaagca ttaccattag
     1081 cagcgaaggc agcaaagcca tgcaggaagg cgcgtatcgt tttattcgta atccgaacgt
     1141 gagcgcggaa gcgattcgta aagcgggtgc catgcagacc gtgaaactgg cccaggaatt
     1201 tccggaactg ctggcaattg aagataccac ctctctgagc tatcgtcatc aggtggcgga
     1261 agaactgggc aaactgggta gcattcagga taaaagccgt ggttggtggg tgcatagcgt
     1321 gctgctgctg gaagcgacca cctttcgtac cgtgggcctg ctgcatcaag aatggtggat
     1381 gcgtccggat gatccggcgg atgcggatga aaaagaaagc ggcaaatggc tggccgctgc
     1441 tgcaacttcg cgtctgagaa tgggcagcat gatgagcaac gtgattgcgg tgtgcgatcg
     1501 tgaagcggat attcatgcgt atctgcaaga taaactggcc cataacgaac gttttgtggt
     1561 gcgtagcaaa catccgcgta aagatgtgga aagcggcctg tatctgtatg atcacctgaa
     1621 aaaccagccg gaactgggcg gctatcagat tagcattccg cagaaaggcg tggtggataa
     1681 acgtggcaaa cgtaaaaacc gtccggcgcg taaagcgagc ctgagcctgc gtagcggccg
     1741 tattaccctg aaacagggca acattaccct gaacgcggtg ctggccgaag aaattaatcc
     1801 gccgaaaggc gaaaccccgc tgaaatggct gctgctgacc agcgagccgg tggaaagtct
     1861 ggcccaagcg ctgcgtgtga ttgatattta tacccatcgt tggcgcattg aagaatttca
     1921 caaagcgtgg aaaacgggtg cgggtgcgga acgtcagcgt atggaagaac cggataacct
     1981 ggaacgtatg gtgagcattc tgagctttgt ggcggtgcgt ctgctgcaac tgcgtgaatc
     2041 ttttactccg ccgcaagcac tgcgtgcgca gggcctgctg aaagaagcgg aacacgttga
     2101 aagccagagc gcggaaaccg tgctgacccc ggatgaatgc caactgctgg gctatctgga
     2161 taaaggcaaa cgcaaacgca aagaaaaagc gggcagcctg caatgggcgt atatggcgat
     2221 tgcgcgtctg ggcggcttta tggatagcaa acgtaccggc attgcgagct ggggtgcgct
     2281 gtgggaaggt tgggaagcgc tgcaaagcaa actggatggc tttctggccg cgaaagacct
     2341 gatggcgcag ggcattaaaa tctgcatcac gggagatgca ctagttgccc tacccgaggg
     2401 cgagtcggta cgcatcgccg acatcgtgcc gggtgcgcgg cccaacagtg acaacgccat
     2461 cgacctgaaa gtccttgacc ggcatggcaa tcccgtgctc gccgaccggc tgttccactc
     2521 cggcgagcat ccggtgtaca cggtgcgtac ggtcgaaggt ctgcgtgtga cgggcaccgc
     2581 gaaccacccg ttgttgtgtt tggtcgacgt cgccggggtg ccgaccctgc tgtggaagct
     2641 gatcgacgaa atcaagccgg gcgattacgc ggtgattcaa cgcagcgcat tcagcgtcga
     2701 ctgtgcaggt tttgcccgcg ggaaacccga atttgcgccc acaacctaca cagtcggcgt
     2761 ccctggactg gtgcgtttct tggaagcaca ccaccgagac ccggacgccc aagctatcgc
     2821 cgacgagctg accgacgggc ggttctacta cgcgaaagtc gccagtgtca ccgacgccgg
     2881 cgtgcagccg gtgtatagcc ttcgtgtcga cacggcagac cacgcgttta tcacgaacgg
     2941 gttcgtcagc cacgctactg gcctcaccgg tctgaactca ggcctcacga caaatcctgg
     3001 tgtatccgct tggcaggtca acacagctta tactgcggga caattggtca catataacgg
     3061 caagacgtat aaatgtttgc agccccacac ctccttggca ggatgggaac catccaacgt
     3121 tcctgccttg tggcagcttc aaaagcttgc ggccgcactc gagcaccacc accaccacca
     3181 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
     3241 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
     3301 aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     3361 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     3421 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3481 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3541 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     3601 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     3661 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     3721 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     3781 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3841 aaatacattc aaatatgtat ccgctcatga attaattctt agaaaaactc atcgagcatc
     3901 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     3961 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     4021 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     4081 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     4141 agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     4201 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
     4261 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     4321 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     4381 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     4441 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     4501 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     4561 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     4621 ccatataaat cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt
     4681 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     4741 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4801 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4861 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4921 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     4981 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5041 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5101 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5161 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5221 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5281 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5341 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5401 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5461 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5521 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5581 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa
     5641 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     5701 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     5761 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5821 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa
     5881 gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct
     5941 cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
     6001 cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg
     6061 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg
     6121 cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga
     6181 gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg
     6241 gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc
     6301 gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg
     6361 cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct
     6421 aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc
     6481 gcacccgtgg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg
     6541 cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca
     6601 ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg
     6661 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag
     6721 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc
     6781 gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg
     6841 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6901 gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac
     6961 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt
     7021 tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg
     7081 tcttcggtat cgtcgtatcc cactaccgag atgtccgcac caacgcgcag cccggactcg
     7141 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga
     7201 acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg
     7261 ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc
     7321 agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga
     7381 cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata
     7441 ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca
     7501 gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg
     7561 cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc
     7621 atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt
     7681 tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg
     7741 cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc
     7801 actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc
     7861 tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc
     7921 accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat
     7981 tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc
     8041 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
     8101 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     8161 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     8221 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga
     8281 gatcgatctc gatcccgcga aat
//
