LOCUS       sequence-248905-        3844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  contains the chaperonin-60 universal target from phytoplasma 
            baceria; used as a control for development of molecular diagnostic 
            assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3844)
  TITLE     Dumonceaux lab phytoplasma plasmids
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SF1
FEATURES             Location/Qualifiers
     source          1..3844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        133..151
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     133..150
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1074..1083
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1078..1087
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1117..1136)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1118..1136)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1143..1160)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1143..1159)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1152..1174)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1300..1755
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1387..1406)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1597..1618
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1833..1937
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1938..2006,2007..2798)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2156..2175)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2969..3557
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3458..3477
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3711..3728
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag ctatttaggt gacactatag aatactcaag ctatgcatcc aacgcgttgg
      181 gagctctccc atatggtcga cctgcaggcg gccgcgaatt cactagtgat tggcaaagaa
      241 gcaagaaaag ctttattaca aggagtagat gcaattgcaa atactgttaa agtgacttta
      301 gggcctaaag gacgtaatgt tattttagaa aaagcctatg attcacctgc tattgtaaat
      361 gatggtgttt ctattgctaa agaaattgaa ttaaaaaatc cttatcaaaa tatgggagca
      421 aagttagtat atgaagtagc ttccaaaact aacgataaag caggagatgg aacaactaca
      481 gcaactgttt tggcacaaag tatgattcat cgtgggtttg atgcaattga tgcaggagct
      541 aatcctgttt tagtaaaaga aggaattgag ttagctgcat taacagttgc caaaaaactt
      601 ttagctaaat ctaaaaaagt agacgcccaa gaagatattc aaaatgtggc tgctgtttca
      661 tcaggtagtc aagaaattgg taaaatcatt gcccaagcga tgcaaaaagt aggaaaagat
      721 ggagttatta atgttgatga atccaaaggt tttgaaacag aattagaagt tgttgaagga
      781 ttgcagtacg ataaaggata tgcttctcct tattttgtct ctgatagaga aagtatgaca
      841 gtacagttag aaaatgcgtt agttttagta actgatcata aaattagtac tgtgcaagaa
      901 attgtaccta ttttggaaga agtagtaaaa gcatctagac ctttattaat tgtagctgaa
      961 gctgtggaaa atgaagtttt aggggttttg gtagctaata aattaagagg aacttttaat
     1021 gtagttgtaa ctaatgcccc tggttttggc gacaaccaaa tcgaattccc gcggccgcca
     1081 tggcggccgg gagcatgcga cgtcgggccc aattcgccct atagtgagtc gtattacaat
     1141 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     1201 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     1261 cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat
     1321 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     1381 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     1441 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     1501 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     1561 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     1621 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     1681 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     1741 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     1801 ccgcatcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1861 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1921 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1981 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     2041 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     2101 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     2161 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     2221 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     2281 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     2341 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     2401 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     2461 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     2521 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     2581 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2641 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2701 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2761 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2821 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2881 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2941 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3001 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3061 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3121 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3181 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3241 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3301 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3361 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3421 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3481 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3541 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3601 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3661 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3721 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3781 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3841 caat
//
