LOCUS       sequence-249007-        2379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes EGFP as a Type 3a part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3a_011
FEATURES             Location/Qualifiers
     source          1..2379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     388..407
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(599..601,602..1312)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     terminator      complement(1332..1366)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1437..2096)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2011..2030
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2278..2379,1..487)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
       61 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      121 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      181 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      241 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      301 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      361 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      421 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      481 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      541 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtagtc ggtctcatat
      601 gtcaaaaggc gaggaactat tcacaggtgt cgtaccaata ctggtagaat tggatggcga
      661 cgtaaacggg cataagtttt ctgtgagtgg tgagggtgaa ggggacgcaa cttatggcaa
      721 acttacactt aagttcatct gcaccacagg gaagctgcct gtcccatggc caactctagt
      781 tacaactctc acttacggag tacaatgttt ttctcgatac ccagaccaca tgaaacaaca
      841 tgactttttc aaatctgcca tgcctgaggg atatgtgcaa gagagaacta ttttctttaa
      901 agatgatggt aattacaaaa ctcgtgctga agtgaagttt gaaggagata cacttgttaa
      961 tagaattgag ttaaagggta ttgactttaa ggaagatggt aatatcttag gtcacaaatt
     1021 ggaatacaac tacaattcac ataacgttta catcatggca gataaacaga aaaacggcat
     1081 aaaagttaat ttcaaaatta gacataatat cgaagatggt tccgttcaac tcgctgatca
     1141 ttatcaacaa aatacaccaa tcggagatgg acctgttcta ttgccagaca accattactt
     1201 atctacacag agcgccttgt caaaagatcc taatgaaaag agagatcaca tggttttgtt
     1261 agaatttgtc accgctgcgg gtataacgtt gggcatggat gaattataca agggttcttg
     1321 agaccagacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
     1381 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac
     1441 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
     1501 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
     1561 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
     1621 tttaaatcaa aactggtgaa actcacccag ggattggctg aaacgaaaaa catattctca
     1681 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     1741 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
     1801 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
     1861 tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
     1921 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
     1981 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
     2041 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
     2101 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     2161 tcattatggt gaaagttgga acctcttacg tgcccgatca atcatgacca aaatccctta
     2221 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2281 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2341 ggtggtttgt ttgccggatc aagagctacc aactctttt
//
