LOCUS       sequence-249064-        1947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes HELB-CDK2 Translocation Reporter as a Type 3a part to be 
            used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3a_021
FEATURES             Location/Qualifiers
     source          1..1947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      236..824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     725..744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1237..1271)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(1342..1947,1..54))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1916..1935
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
       61 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
      121 attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac
      181 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
      241 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      301 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      361 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      421 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      481 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      541 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      601 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      661 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      721 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      781 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      841 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      901 cccctgattc tgtggataac cgtagtcggt ctcatatgac aaatgatgtc acctggagcg
      961 aggcctcttc gcctgatgag aggacactca cctttgctga aagatggcaa ttatcttcac
     1021 ctgatggagt agatacagat gatgatttac caaaatcgcg agcatccaaa agaacctgtg
     1081 gtgtgaatga tgatgaaagt ccaagcaaaa tttttatggt gggagaatct ccacaagtgt
     1141 cttccagact tcagaatttg agactgaata atttaattcc caggcaactt ttcaagccca
     1201 ccgataatca agaaactggt tcttgagacc agaccaataa aaaacgcccg gcggcaaccg
     1261 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     1321 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa
     1381 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
     1441 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     1501 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     1561 ggctgaaacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     1621 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     1681 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     1741 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt
     1801 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     1861 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     1921 tgactgaaat gcctcaaaat gttcttt
//
