LOCUS       sequence-249099-        2724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes bacterial light-activated adenylyl cyclase (bPAC) as a Type 
            3b part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3b_007
FEATURES             Location/Qualifiers
     source          1..2724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      56..644
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     545..564
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1834..1868)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1939..2598)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2513..2532
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
       61 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      721 cccctgattc tgtggataac cgtagtcggt ctcattctaa gcggctggtg tacatcagca
      781 agatcagcgg ccacctgagc ctggaagaga tccagcggat cggcaaggtg tccatcaaga
      841 acaaccagcg ggacaacatc accggcgtgc tgctgtacct gcagggcctg ttcttccaga
      901 tcctggaagg cgagaacgag aaggtggaca agctgtacaa gaaaatcctg gtggacgacc
      961 ggcacaccaa catcctgtgc ctgaaaaccg agtacgacat caccgaccgg atgttcccca
     1021 actgggccat gaaaaccatc aacctgaacg agaacagcga gctgatgatc cagcccatca
     1081 agagcctgct gcagaccatc acccagagcc accgggtgct ggaaaagtac atgcccgcca
     1141 gagtgatcta cctgatcaac cagggcatca accccctgac cgtggagccc cagctggtcg
     1201 agaagatcat cttcttcagc gacatcctgg ccttcagcac cctgaccgag aagctgcccg
     1261 tgaacgaggt ggtcatcctg gtcaaccggt acttcagcat ctgcacccgg atcatcagcg
     1321 cctacggcgg cgaagtgacc aagttcatcg gcgactgcgt gatggccagc ttcaccaaag
     1381 agcagggcga cgccgccatc cggaccagcc tggacatcat ctccgagctg aagcagctgc
     1441 ggcaccacgt ggaggccacc aaccccctgc acctgctgta caccggcatc ggcctgagct
     1501 acggccacgt gatcgagggc aacatgggca gcagcctgaa gatggaccac accctgctgg
     1561 gggacgccgt gaacgtggcc gccaggctgg aagccctgac aagacagctg ccctacgccc
     1621 tggcctttac agccggcgtg aagaagtgct gccaggccca gtggaccttc atcaacctgg
     1681 gagcccacca ggtcaagggc aagcaggaag ccatcgaggt ctacaccgtg aatgaggccc
     1741 agaagtacta cgacaccctg cagatcaccc agctgatccg gcagaccctg gaaaacgaca
     1801 agccgcggac gtacggatcc tgagaccaga ccaataaaaa acgcccggcg gcaaccgagc
     1861 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
     1921 agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc
     1981 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
     2041 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
     2101 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
     2161 tgaaacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
     2221 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
     2281 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
     2341 atcccatatc accagctcac cgtctttcat tgccatacga aattccggat gagcattcat
     2401 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
     2461 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
     2521 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     2581 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
     2641 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat
     2701 caatcatgac caaaatccct taac
//
