LOCUS       sequence-249118-        3637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses wild type R bodies with mNeonGreen-tagged second copy of 
            RebB under a weak RBS downstream as well as soluble mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Polka JK, Silver PA
  TITLE     A Tunable Protein Piston That Breaks Membranes to Release 
            Encapsulated Cargo.
  JOURNAL   ACS Synth Biol. 2016 Feb 3.
  PUBMED    26814170
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Feb 3."
COMMENT     Sequence Label: Reb_206 aka PAS629
FEATURES             Location/Qualifiers
     source          1..3637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3478..3498
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     primer_bind     complement(3551..3569)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3565..3612
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 agagaatcag tacacacttt aaattaattc aaaacaagga gctcaaaatg gcgactacta
       61 cttctactgc tgatgtaggc aacacaactg acatcgtaaa tagccaaatt actgattcag
      121 taacacaaac taacacaatg gttcttggtt ctactccagc acaaatcatg acaaatttaa
      181 tgcaaatgag cgcacaagct aatgctttag caatgcaaaa tgctgttgtt ggtcaaaaac
      241 aaacaaatat gctatcagat gcagcaacca ctcaaggctt atctattatg tatacgcttt
      301 cagctgcttc agatggtcaa gcagtcagta ctgttgacaa ttcaactgac atggctggac
      361 tgattgatgc gcttggcgtt gataaagcat aagtattaaa aatagattat ttataaaaca
      421 ccgtaagata aaatcttacg atttaaaacc cagacaaagt aaggaataaa aaatgagtaa
      481 tgtaaattca caaattacag attcagtaac acaaacaaac actaaaatat taggtgaaat
      541 gcctgcgttt accactggaa gcttaatgca aatggcaaca caagctgctg gcttatctat
      601 acaaaactct gtaaccaatc agcaacaatc taatatgctt catcaggcat caaccactca
      661 aggtatgagt attctatatt ctgttgacac ggcagctaat gctcaagcca ttggttcagt
      721 gaaccgttct aatgacacaa gccgtttaac tgatgccctt gctgttatta aagccgctaa
      781 aaatggttaa tgcttatttc gatatgctaa aataatgcca atcagataaa ttggctaaat
      841 taaagtatga taagcgaaat aaaatcattt aaagtagata aaaatcatca gagacggtta
      901 attgttctcg tgggggaaga aagctggggt taggaagtat acgttgattt gtattattga
      961 gtttctcctc aacgcataac gtattacttc ctgacccaac gctatcgttt tccttatttt
     1021 atttttttat tttgcttttt gatatcaatc cattttaact agagcttcaa actatggcag
     1081 ataatgcaat aacacccaat ctttatgtga atccacaagt gaccgattct gtcacttcaa
     1141 atatgatgaa tgttataggt atggcaccag ctcaagcaat gggtgtgctg tatcaaggcg
     1201 ttgcttcaac cgctaattta gctattcaaa atgctcaatc aagctctcaa cagttaaatc
     1261 aaatcggtca agccgtcact tctgttgcct gtgaacgtat tatgaaaatg atgggataat
     1321 cttgtaaatg gcggatatag aacatttctc agaagatatc aatcaacgga tgatggatcc
     1381 taaaaataat gtcgcctctc aaggtgctta tgatattgtt tcaatggctg ccgctagcgc
     1441 tgtgcaaaat gaactggcta tgcattctgg ctttgaaaca acatcaaata cggtgctagc
     1501 acttgctatg caaaaaatgg ctgaagctgc cgcaatcgac aacgaacctc aaattaaaat
     1561 atgggaagga atcatgaaat ccattagcag taatcgagat gctgaagtgg ctaatctcag
     1621 taaatttaat gagatgacac aaaacttagt gaaaaacttt ccacgaggct aaaggaggta
     1681 aaaaatggtt agtaaaggag aagaaaataa catggcaatc attaaggagt tcatgagatt
     1741 caaagttcac atggaaggtt ctgtaaatgg acatgaattt gaaatagaag gtgaaggaga
     1801 aggaaggcct tatgaaggaa cccaaaccgc gaagctaaaa gttactaagg gtggcccatt
     1861 accatttgca tgggatatcc ttagccctca attcatgtat gggtcaaagg cttatgtcaa
     1921 gcaccccgcc gacattccag actatctaaa gttatctttt cccgaagggt ttaagtggga
     1981 gcgtgtgatg aacttcgaag acggtggcgt ggtaacagtg actcaggatt cgtccctgca
     2041 agatggtgaa tttatctaca aagtcaaatt aagaggaact aactttccat ctgacggccc
     2101 ggttatgcaa aaaaagacaa tgggctggga ggcctcctca gaacgaatgt accctgaaga
     2161 tggtgccttg aagggtgaga ttaaacaaag attgaaattg aaagatggtg gacattatga
     2221 cgctgaggtt aaaacgacat acaaagctaa gaaacctgtc cagctcccag gtgcttacaa
     2281 tgtaaatata aaacttgata ttacatcaca taatgaagat tatacgatag ttgaacaata
     2341 cgaaagggct gaggggagac atagtactgg tggcatggat gaactataca aaggttctgg
     2401 tacagcgtaa ctcgagtcac acaggactac tagatggtta gtaagggtga ggaagacaat
     2461 atggcaagcc tgccggcaac gcacgaactg catatctttg gttcaatcaa cggcgtggac
     2521 tttgacatgg tcggccaggg gactggtaac ccgaacgatg gatacgagga actgaactta
     2581 aaatcgacaa aaggagatct gcaattctcg ccatggattc tcgtaccgca tattgggtac
     2641 ggctttcacc agtatttgcc gtatccagat ggcatgagtc cgtttcaagc cgctatggtc
     2701 gatggtagcg gctatcaggt gcatcgcact atgcagtttg aagacggcgc cagtttgaca
     2761 gtcaattacc gttacactta tgaaggctct catattaaag gtgaggcgca agttaagggt
     2821 accgggtttc ccgccgacgg tcccgtaatg actaatagct taaccgcggc tgattggtgc
     2881 cgtagcaaaa aaacctaccc gaacgacaaa accatcattt ccacgtttaa atggagctat
     2941 actacaggta acgggaagcg ctatcgctca acggcgcgca ctacatacac gttcgcgaaa
     3001 ccgatggccg cgaattacct caaaaaccag ccgatgtatg tatttcgtaa aaccgagctg
     3061 aaacatagca agacagaact gaactttaaa gagtggcaga aagcattcac agacgtcatg
     3121 ggaatggatg aactttacaa gtcaggccaa ggatccggca tgagtaatgt aaattcacaa
     3181 attacagatt cagtaacaca aacaaacact aaaatattag gtgaaatgcc tgcgtttacc
     3241 actggaagct taatgcaaat ggcaacacaa gctgctggct tatctataca aaactctgta
     3301 accaatcagc aacaatctaa tatgcttcat caggcatcaa ccactcaagg tatgagtatt
     3361 ctatattctg ttgacacggc agctaatgct caagccattg gttcagtgaa ccgttctaat
     3421 gacacaagcc gtttaactga tgcccttgct gttattaaag ccgctaaaaa tggttaacac
     3481 caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg
     3541 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     3601 aggggttttt tgctgaaagg aggaactata tccggat
//
