LOCUS       sequence-249154-        2859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes rat Connexin 43 as a Type 3b part to be used in the MTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3b_017
FEATURES             Location/Qualifiers
     source          1..2859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1993..2027)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2098..2757)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2672..2691
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 caatcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtagt cggtctcatt
      781 ctggactccg ctctcgtgct cagggtgact ggagtgcctt ggggaagctt ctggacaagg
      841 tccaagccta ctccaccgct ggagggaagg tgtggctgtc agtgctcttc atattcagaa
      901 tcctgctcct ggggacagct gttgagtcag cttggggtga tgaacagtct gcctttcgct
      961 gtaacactca acaacctggc tgcgaaaacg tctgctatga caagtccttc cccatctctc
     1021 acgtgcgctt ctgggtcctt cagatcatat tcgtgtctgt gcccaccctc ctgtacttgg
     1081 cccatgtgtt ctatgtgatg aggaaggaag agaagctaaa caagaaagaa gaggagctca
     1141 aagtggccca gactgacggg gtcaacgtgg agatgcacct gaagcagatt gaaatcaaga
     1201 agttcaagta cgggattgaa gagcacggca aggtgaaaat gaggggcggc ttgctgagaa
     1261 cctacatcat cagcatcctc ttcaagtctg tcttcgaggt ggccttcctg ctcatccagt
     1321 ggtacatcta tgggttcagc ttgagcgcgg tctacacctg caagagagat ccctgccccc
     1381 accaggtaga ctgcttcctc tcacgtccca cggagaaaac catcttcatc atcttcatgc
     1441 tggtggtgtc cttggtgtct ctcgctttga acatcattga gctcttctac gtcttcttca
     1501 aaggcgttaa ggatcgcgtg aagggaagaa gcgatcctta ccacgccacc actggcccac
     1561 tgagcccatc aaaagactgc ggatctccaa aatacgccta cttcaatggc tgctcctcac
     1621 caacggctcc actctcgcct atgtctcctc ctgggtacaa gctggttact ggtgacagaa
     1681 acaattcctc gtgccgcaat tacaacaagc aagctagcga gcaaaactgg gcgaactaca
     1741 gcgcagagca aaatcgcatg gggcaggccg gaagcaccat ctccaactcg cacgcccagc
     1801 cgttcgattt ccccgacgac aaccagaatg ccaaaaaagt tgctgctgga catgaactcc
     1861 agccattagc catcgtggac caacgacctt ccagcagagc cagcagccgc gccagcagca
     1921 ggcctcggcc tgatgacctg gagatttccg cccgggatcc accggtcgcc accggatcct
     1981 gagaccagac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     2041 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
     2101 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     2161 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     2221 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     2281 gtttaaatca aaactggtga aactcaccca gggattggct gaaacgaaaa acatattctc
     2341 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     2401 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     2461 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     2521 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
     2581 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     2641 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     2701 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     2761 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     2821 ttcattatgg tgaaagttgg aacctcttac gtgcccgat
//
