LOCUS       sequence-249188-        6171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 400 / 504, Emission: 515 / 
            517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6171)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-Fascin-C-10
FEATURES             Location/Qualifiers
     source          1..6171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        63..366
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        367..570
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     521..541
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      609..618
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             615..1322
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(760..778)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    2961..3082
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2998..3017)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3052..3071
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3089..3544)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3226..3247)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3438..3457
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3571..3675
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3677..4034
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3677..3868
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3700..3720)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3885..4020
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4069..4863
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4069..4863
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4123..4142)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4733..4752
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5051..5070)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5095..5142
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5471..6059
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5960..5979
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      541 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
      601 taccggtcgc caccatggtg agcaagggcg aggaggataa catggccatc attaaggagt
      661 tcatgcgctt caaggtgcac atggaggggt ccgtgaacgg ccacgtgttc gagatcgagg
      721 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      781 gcggccccct gcccttcacc tgggacatcc tgagccctca gttcatgtac ggctccaatg
      841 cctacgtgaa gcaccccgcc gacatccccg actactttaa gctgtccttc cccgagggct
      901 tcaagtggga gcgcgtgatg aaattcgagg acggcggcgt ggtgaccgtg acccaggact
      961 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     1021 ccgacggccc cgtgatgcag aagaagacca tgggctggga ggccctctcc gagcggatgt
     1081 accccgagga cggcgccctg aagggcgagg tcaagcccag agtgaagctg aaggacggcg
     1141 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     1201 gcgcctacaa cgtcaaccgc aagctggaca tcaccagcca caacgaggac tacaccatcg
     1261 tggagcagta cgagagagcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     1321 agtccggagc tggtagtggt ggaagcgcag gaaccgccaa cggcacagcc gaggcggtgc
     1381 agatccagtt cggcctcatc aactgcggca acaagtacct gacggccgag gcgttcgggt
     1441 tcaaggtgaa cgcgtccgcc agcagcctga agaagaagca gatctggacg ctggagcagc
     1501 cccctgacga ggcgggcagc gcggccgtgt gcctgcgcag ccacctgggc cgctacctgg
     1561 cggcggacaa ggacggcaac gtgacctgcg agcgcgaggt gcccggtccc gactgccgtt
     1621 tcctcatcgt ggcgcacgac gacggtcgct ggtcgctgca gtccgaggcg caccggcgct
     1681 acttcggcgg caccgaggac cgcctgtcct gcttcgcgca gacggtgtcc cccgccgaga
     1741 agtggagcgt gcacatcgcc atgcaccctc aggtcaacat ctacagcgtc acccgtaagc
     1801 gctacgcgca cctgagcgcg cggccggccg acgagatcgc cgtggaccgc gacgtgccct
     1861 ggggcgtcga ctcgctcatc accctcgcct tccaggacca gcgctacagc gtgcagaccg
     1921 ccgaccaccg cttcctgcgc cacgacgggc gcctggtggc gcgccccgag ccggccactg
     1981 gctacacgct ggagttccgc tccggcaagg tggccttccg cgactgcgag ggccgttacc
     2041 tggcgccgtc ggggcccagc ggcacgctca aggcgggcaa ggccaccaag gtgggcaagg
     2101 acgagctctt tgctctggag cagagctgcg cccaggtcgt gctgcaggcg gccaacgaga
     2161 ggaacgtgtc cacgcgccag ggtatggacc tgtctgccaa tcaggacgag gagaccgacc
     2221 aggagacctt ccagctggag atcgaccgcg acaccaaaaa gtgtgccttc cgtacccaca
     2281 cgggcaagta ctggacgctg acggccaccg ggggcgtgca gtccaccgcc tccagcaaga
     2341 atgccagctg ctactttgac atcgagtggc gtgaccggcg catcacactg agggcgtcca
     2401 atggcaagtt tgtgacctcc aagaagaatg ggcagctggc cgcctcggtg gagacggcag
     2461 gggactcaga gctcttcctc atgaagctca tcaaccgccc catcatcgtg ttccgcgggg
     2521 agcatggctt catcggctgc cgcaaggtca cgggcaccct ggacgccaac cgctccagct
     2581 atgacgtctt ccagctggag ttcaacgatg gcgcctacaa catcaaagac tccacaggca
     2641 aatactggac ggtgggcagt gactccgtgg tcaccagcag cggcgacact cctgtggact
     2701 tcttcttcga gttctgcgac tataacaagg tggccatcaa ggtgggcggg cgctacctga
     2761 agggcgacca cgcaggcgtc ctgaaggcct cggcggaaac cgtggacccc gcctcgctct
     2821 gggagtacta gggatccacc ggatctagat aactgatcat aatcagccat accacatttg
     2881 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     2941 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     3001 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3061 ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc
     3121 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     3181 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     3241 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     3301 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     3361 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     3421 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     3481 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     3541 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3601 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3661 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     3721 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     3781 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3841 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     3901 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     3961 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     4021 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     4081 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     4141 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     4201 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     4261 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     4321 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     4381 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     4441 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     4501 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     4561 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     4621 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     4681 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     4741 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     4801 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     4861 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     4921 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     4981 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     5041 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     5101 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     5161 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     5221 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     5281 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt
     5341 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5401 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5461 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5521 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5581 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5641 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5701 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5761 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5821 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5881 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5941 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6001 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6061 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6121 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg c
//
