LOCUS       sequence-24924-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Oka T, Mazack V, Sudol M
  TITLE     Mst2 and Lats kinases regulate apoptotic function of Yes 
            kinase-associated protein (YAP).
  JOURNAL   J Biol Chem. 2008 Oct 10;283(41):27534-27546. doi: 
            10.1074/jbc.M804380200. Epub 2008 Jul 17.
  PUBMED    18640976
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M804380200"; journalName: "J Biol Chem"; date: 
            "2008-10-10- 10"; volume: "283"; issue: "41"; pages: "27534-27546"
COMMENT     Sequence Label: pcDNA4/HisMaxB-YAP1-S103A
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    24..187
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             200..217
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             221..253
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(257..265,266..280)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 cacngctncg tttacttaag cttagcgcag aggcttgggg cagccgagcg gcagccaggc
       61 cccggccccc nttctcggtt ccagaaggga gaggagcccg ccaaggcgcg caagagagcg
      121 ggctgcctcg cagtccgagc cggagaggga gcgcgagccg cgccggcccc ggacggcctc
      181 cgaaaccatg gggggttctc atcatcatca tcatcatggt atggctagca tgactggtgg
      241 acagcaaatg ggtcgggatc tgtacgacga tgacgataag gtacctaagg atcccgggca
      301 gcagccgccg cctcaaccgg ccccccaggg ccaagggcag ccgccttcgc agcccccgca
      361 ggggcagggc ccgccgtccg gacccgggca accggcaccc gcggcgaccc aggcggcgcc
      421 gcaggcaccc cccgccgggc atcagatcgt gcacgtccgc ggggactcgg agaccgacct
      481 ggaggcgctc ttcaacgccg tcatgaaccc caagacggcc aacgtgcccc agaccgtgcc
      541 catgaggctc cggaagctgc ccgactcctt cttcaagccg ccggagccca aagcccactc
      601 ccgacaaggc aantantgat tcaggcactg canganccct gactccacag catgttcgag
      661 ctcattnctt ctcantttnt ntnnanttgg aatttttttt tcttggaaat tnacccnttt
      721 ganatatttt tttctggcca ntactaacca nagttttnga tctttcaaag tcttctttga
      781 annncttgat natgtactct gcancanntn gganatgcaa gaatcttngg tca
//
