LOCUS       sequence-249435-       13616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PPI (BiFC/TriFC) assay vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13616)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 13616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-494    N-Tag GFP[2] L10 Piezo1 EBPF2
FEATURES             Location/Qualifiers
     source          1..13616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        153..532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        533..736
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     687..707
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     733..757
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          872..1004
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     regulatory      1019..1028
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(8775..9349,9350..9352,9353..9361)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8942..8959)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9169..9188
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(9362..9364,9365..9367,9368..10081)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(9407..9428)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     intron          10127..10259
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    10268..10856
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10321..10341)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10739..10750)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10883..10900)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10889..11000
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11096..11116)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11096..11114)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11135..11151)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11135..11151)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11148..11170)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     complement(11253..11272)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    11318..11373
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(11372..11391)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(11752..12340)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11832..11851)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12511..13302,13303..13371))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13134..13153
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13372..13476)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13544..13562
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcctgcctcc aggcctcaat attggccatt agccatatta ttcattggtt atatagcata
       61 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
      121 tattggctca tgtccaatat gaccgccatg ttggcattga ttattgacta gttattaata
      181 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      241 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      301 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      361 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
      421 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
      481 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      541 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      601 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      661 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
      721 ctatataagc agagctcgtt tagtgaaccg tcagatcact agaagcttta ttgcggtagt
      781 ttatcacagt taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag
      841 ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta
      901 aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc
      961 acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccaggc
     1021 caccatggaa aagagggacc acatggtgct gctcgagtac gtcacagcag ctggcatcac
     1081 cgatgccagc gggagctctg ccagcggtgg cagcacctcc gagccgcacg tgctgggcgc
     1141 cgggctctac tggctgttgc tgccctgcac gctcctggcg gcctccctgt tacgcttcaa
     1201 tgctctctcg ctggtctatt tgctgtttct actgctgctg ccctggcttc caggcccctc
     1261 aagacacagc ataccaggtc acacaggtcg cctgctccgt gcactactct gcctcagcct
     1321 cctcttcctg gtggcccacc ttgcctttca gatatgccta cacaccgtgc ctcacctgga
     1381 ccagtttctg ggacaaaacg gtagcctttg ggtgaaggtg tctcaacaca taggggttac
     1441 gaggctggac ctgaaggaca tctttaacac caccaggctg gtagcacctg acctgggagt
     1501 gctgctggcg tcctcccttt gccttggcct ctgtggacgc ctcacgagga aagccgggca
     1561 gagtcggcgc acccaggagc tgcaggatga tgacgatgac gatgatgacg acgatgaaga
     1621 catagatgct gccccagccg tggggctgaa gggagcccct gccctggcaa ccaaacgcag
     1681 gctgtggctg gcctcccgct tccgggtcac ggcccactgg ctgctgatga cctctggacg
     1741 gacgctggtc attgtgttgc tggccctggc aggcatagcc cacccttcgg ccttctccag
     1801 catctacctg gtggtgttcc tggccatctg cacctggtgg tcctgtcact ttcctctcag
     1861 ccccctgggc tttaacaccc tctgtgtcat ggtgagctgt tttggtgccg gccatctcat
     1921 ttgcctatac tgctatcaga caccatttat ccaggacatg ttaccccctg ggaacatctg
     1981 ggccaggcta tttggtctca agaacttcgt agacctccct aactactcca gccccaacgc
     2041 cctggtactc aacactaagc acgcctggcc catctatgtg agtcccggaa tcctgctgct
     2101 gctatattac acagccacct ctctcctgaa gctccacaag agctgtccct cagagctgag
     2161 gaaggagaca cccagggagg atgaggagca tgagctggaa ctggaccacc tggagccaga
     2221 gccccaggct agggacgcca cccagggtga gatgcccatg accacggaac ctgaccttga
     2281 caactgcacc gtgcatgtac taaccagcca gagccctgtc cgccagcgtc cagttcgccc
     2341 caggctggct gagctgaaag agatgtcacc gctacatggc ctgggccacc tcatcatgga
     2401 ccagagctat gtgtgtgccc tgattgccat gatggtgtgg agcatcatgt accacagttg
     2461 gctgaccttc gtcctgctgc tctgggcctg cctcatctgg acggtgcgta gccgtcacca
     2521 gctggctatg ctctgctcgc cctgcatcct gctgtatggg ctgacgctct gctgcctgag
     2581 gtatgtgtgg gccatggaac ttcctgagct gcccaccacc ctgggccctg tcagcctgca
     2641 ccagttggga ctggaacaca cacgctaccc ttgcctggac ctcggtgcca tgctcctcta
     2701 tctgctcaca ttctggctcc ttctgcgtca gttcgtgaag gagaagctgc tgaagaagca
     2761 aaaggtgcct gcggcgctgc tggaggtcac ggtggccgac actgagccca cacagaccca
     2821 gacgctgctg cggagcctgg gggagctggt caccggcatc tacgtcaaat actggatcta
     2881 tgtgtgcgcc ggcatgttca ttgtggtcag cttcgccggc cgcctggtgg tctacaaaat
     2941 cgtctacatg ttcctcttcc tgctgtgcct caccttgttc caggtctact acaccctgtg
     3001 gaggaagctg ctacgtgtct tctggtggct cgtggtggcc tatacaatgc tcgtgctcat
     3061 cgctgtgtac accttccagt tccaggactt ccccacctat tggcgcaacc tcacgggctt
     3121 cacagacgag cagttgggcg acctgggcct ggagcagttc agtgtgtcgg agctcttttc
     3181 cagtatcctc atccctggct tcttcctgct ggcctgcatc ctgcagctgc actacttcca
     3241 cagaccgttc atgcagctca ctgacctgga gcacgtgccg ccaccaggca cccgccaccc
     3301 tcgatgggct cacaggcagg atgcagtgag cgaggcccct ctgcttgagc atcaggagga
     3361 agaggaagtc ttcagggaag atgggcagag catggatggg ccccaccagg ccacacaggt
     3421 ccctgagggt acggccagca agtggggcct ggtggctgac cggctgctgg acctagcggc
     3481 cagcttctcg gctgtcctca cccgaatcca ggtgttcgtt cggcgcttgc tagaacttca
     3541 cgtcttcaag ctggtggccc tctacactgt ctgggtggcc ctgaaagaag tgtctgtgat
     3601 gaacctgctg ctggtggtgc tatgggcctt cgccttgccc tatccgcgct tccggcccat
     3661 ggcttcctgc ctgtccaccg tgtggacctg tatcatcatt gtatgcaaga tgctctatca
     3721 gctcaagatt gtcaacccgc atgagtactc cagcaactgc actgagccct tccccaacaa
     3781 taccaacttg cagcctttgg agatcaacca gtctttgctg taccgtggcc ctgttgaccc
     3841 tgccaactgg tttggggtgc ggaagggtta ccccaacttg ggctatatcc agaaccacct
     3901 gcagatcctt ctgttgctgg tgtttgaggc cgtggtgtac cggcgccaag agcactaccg
     3961 ccggcagcac cagcaggccc ctctgcccgc ccaggctgtg tgcgcagatg gcacccgcca
     4021 gaggttggac caggacctac ttagctgcct caagtatttc atcaacttct tcttctacaa
     4081 attcgggctg gagatctgct tcttgatggc cgtgaatgtg attgggcagc gtatgaactt
     4141 catggtgatc ttacacggtt gctggttggt ggccatcctt acacgccggc gccgtgaggc
     4201 catcgcccgc ctctggccta actactgtct gttcctcacg ctgttcctgc tgtaccagta
     4261 cctgctgtgt ttgggcatgc cccctgcact gtgcattgac tatccatggc gctggagcaa
     4321 ggccatcccc atgaattccg ccctcatcaa gtggctgtac ctacctgact tcttcagagc
     4381 ccccaactcc accaacctta tcagtgactt cctcctgctg ctttgcgcct cccagcagtg
     4441 gcaggtcttc tcagcggagc gaacggagga gtggcaacgc atggcgggca tcaacactga
     4501 ccacctggag cccctgcgtg gggagcccaa ccctataccc aacttcatcc actgcaggtc
     4561 ctatctggat atgctgaagg tggccgtctt ccgctacctg ttctggctgg tgctcgttgt
     4621 ggtgtttgtt gcgggggcca cccgcataag catcttcggg ctggggtacc tgctagcctg
     4681 cttctacctg ctgctgtttg gcactaccct gctgcagaag gacacgcgag cccagctcgt
     4741 gctgtgggac tgcctcatcc tctataatgt cactgtcatc atctctaaga atatgctgtc
     4801 gctcctgtcc tgtgtcttcg tggagcaaat gcagagcaac ttctgctggg tcatccagct
     4861 cttcagcctc gtgtgcacag tcaaaggcta ctatgatccc aaagagatga tgaccaggga
     4921 ccgggactgc ctgctgcctg tggaggaggc cgggatcatc tgggacagta tctgcttctt
     4981 cttcctgctc ttgcaacggc gcatctttct cagccactac ttcctgcatg tcagcgctga
     5041 cctgaaagcc acagccctgc aggcatccag gggctttgcc ctctacaatg cagccaacct
     5101 gaagagcatc aacttccatc gccagattga ggagaagtcc ctggcccagc tgaaaagaca
     5161 gatgaagcgc atccgtgcca aacaggagaa gtacaggcag agccaggcaa gtcgtggcca
     5221 actccagtcc aaagaccctc aggatcccag ccaggagcca gggcctgaca gcccaggggg
     5281 ctcctccccg ccacggagac agtggtggcg cccctggctg gaccacgcca cagtcatcca
     5341 ctctggcgac tacttcctgt ttgagtcaga tagcgaggag gaagaggagg ccctacctga
     5401 ggaccccagg cctgcagctc agagtgcctt ccagatggca taccaggcat gggtaaccaa
     5461 tgcccagaca gtgctgaggc agcgtcggga gcgggcacgg caggagcggg cagagcagct
     5521 ggcttctgga ggtgacttga acccagatgt ggaaccagta gatgtcccag aagatgagat
     5581 ggcaggccgt agccacatga tgcagcgtgt gctaagcacc atgcagttcc tgtgggtgct
     5641 gggccaggcc acggtagacg ggctgacgcg ctggctgcgt gcattcacga agcaccaccg
     5701 caccatgagc gatgtgctgt gcgcagagcg ctacctgctc acccaggagc ttcttcgggt
     5761 tggagaggta cgccgaggtg tgctggacca gctttatgtg ggtgaagatg aggccacatt
     5821 gtcaggtccc gtggagaccc gggatggacc cagcacagcc tcaagtgggc tgggagccga
     5881 agagcctttg agtagcatga cagacgacac cagcagcccc ctgagcacag gctataacac
     5941 ccgcagtggc agtgaggaga ttgtcaccga cgctggggac ctccaggctg ggacctccct
     6001 gcacggctcc caagagcttt tagccaatgc tcgtacccgg atgcgcacgg ccagcgagct
     6061 gctactggat aggcgcctgc atatccctga gctggaggag gccgagcggt ttgaggcaca
     6121 gcagggccgg actctgcggc tgctcagggc tgggtaccag tgcgtggcgg cacactcgga
     6181 gctgctctgt tacttcatca tcatccttaa ccacatggtg acagcctcgg ctgcctccct
     6241 ggtgctgccc gtgcttgtgt tcctgtgggc catgctgacc atcccgaggc ctagcaagcg
     6301 cttttggatg acagctatcg tcttcactga ggtcatggtg gtcaccaaat acctgttcca
     6361 gttcggcttc ttcccctgga acagctacgt tgtgctgcgg cgctatgaga acaagcccta
     6421 cttccctccg cgaatcctgg gccttgagaa aacggacagc tacatcaagt atgacctggt
     6481 gcagctcatg gccctcttct tccaccgctc gcagctactg tgttatggcc tctgggacca
     6541 tgaggaggat cgctatccca aggaccattg caggagtagt gtgaaggacc gggaggccaa
     6601 ggaagagcca gaagctaagc tggaatcgca gtctgagaca ggcactgggc atcccaagga
     6661 gccagtgttg gccggtactc ccagggacca catccaaggg aaaggaagta ttagatccaa
     6721 ggatgttatc caagatcccc cagaggacct taagccccgg cacacgaggc acatcagcat
     6781 acgcttcagg aggcgcaagg agactccagg acccaaagga acagcagtca tggagactga
     6841 gcacgaggag ggagaaggaa aagaaactac agagagaaag aggccgcgtc acactcaaga
     6901 aaaatcgaag tttcgggaga gaatgaaggc agctgggcgc cggctgcaga gcttctgtgt
     6961 gtcactggcc cagagcttct accaaccctt gcagcgtttc ttccatgaca ttctgcacac
     7021 aaagtaccgg gcggccaccg acgtctacgc cctcatgttc ctggccgata ttgtcgacat
     7081 catcatcatc atctttggtt tttgggcttt tgggaagcac tctgcagcca cagacattgc
     7141 atcctcgctg tcagatgacc aggtgccaca ggccttcctg ttcatgctgc tggtccagtt
     7201 tggcaccatg gtcatcgacc gtgccctcta cctgcgcaag actgtcctgg gaaagctggc
     7261 ctttcaggtg gtcctggtgg tggcgattca catctggatg ttctttatct taccggctgt
     7321 cactgagagg atgttcagcc agaatgcggt ggcacagctg tggtacttcg tcaagtgcat
     7381 ttactttgcc ctgtccgcct accagatccg ctgtggctac cccacccgta tcttgggcaa
     7441 cttcctcacc aagaaataca accatctaaa cctcttcctt ttccaggggt tccgtctagt
     7501 gccgttcctg gtggagctgc gggccgtcat ggactgggtg tggaccgaca ccacgctgtc
     7561 cctgtccaac tggatgtgtg tggaagacat ctatgccaac atctttatca tcaagtgcag
     7621 ccgagagaca gagaagaaat acccccagcc caaggggcag aagaagaaga aaattgtcaa
     7681 gtatggtatg ggaggcctca ttatcctctt cctcatcgcc atcatctggt tccctctgct
     7741 cttcatgtca ctgatccgct ctgtggtcgg ggtcgtcaac cagcccattg atgtcaccgt
     7801 caccctcaag ctaggcggct atgagccact gttcaccatg agcgcccagc agccatccat
     7861 tgtgccattc acaccccagg cctacgagga gctgtcccag cagtttgacc cctatccact
     7921 agccatgcag ttcattagcc agtacagtcc tgaggacatc gtcactgcac agatcgaggg
     7981 cagctcgggg gcgctgtggc gcatcagccc acccagccga gcccagatga agcaggagct
     8041 gtacaacggc acagccgaca ttacactgcg ctttacctgg aatttccaaa gggacctggc
     8101 caagggtggc actgtggagt atactaatga gaagcacacc ttggagctgg cccccaacag
     8161 tacggcacga aggcagctgg cccaactgct cgagggcaga cctgaccagt cagtggtcat
     8221 tccccatctc ttccccaagt acattcgtgc tcccaatggg cctgaagcca accctgtgaa
     8281 gcagctgcag ccagatgagg aagaggacta ccttggtgtg cgcatccagc tgcggaggga
     8341 gcaagtgggc acaggggcct ctggggagca agcgggcacc aaggcctccg acttcctcga
     8401 gtggtgggtc atcgagctgc aggactgcaa ggctgactgc aacctgctgc ccatggtcat
     8461 cttcagtgac aaggtcagcc cacctagcct gggcttcctg gccggctacg ggattgtggg
     8521 gctgtatgtc tccatcgtgc tggtggttgg caagtttgtg cggggcttct tcagcgagat
     8581 ctctcactcc atcatgttcg aggaactgcc gtgtgtggac cgcatcctca agctgtgcca
     8641 ggacatcttc ttggtgcgcg agacccggga gctggagctg gaggaggagc tatacgccaa
     8701 gctcatcttc ctgtaccgat ctccagagac catgattaag tggacacgtg agagggagta
     8761 agaattcaga tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
     8821 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     8881 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     8941 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggta
     9001 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     9061 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     9121 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     9181 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     9241 ctggggcctc ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     9301 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac
     9361 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     9421 cggcgacgta aacggccaca agttcagcgt gaggggcgag ggcgagggcg atgccaccaa
     9481 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     9541 cctcgtgacc accctgagcc acggcgtgca gtgcttcgcc cgctaccccg accacatgaa
     9601 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     9661 cttcaaggac gacggcacct acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     9721 ggtgaaccgc atcgagctga agggcgtcga cttcaaggag gacggcaaca tcctggggca
     9781 caagctggag tacaacttca acagccacaa catctatatc atggccgtca agcagaagaa
     9841 cggcatcaag gtgaacttca agatccgcca caacgtggag gacggcagcg tgcagctcgc
     9901 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacagcca
     9961 ctacctgagc acccagtccg tgctgagcaa agaccccaac gagaagcgcg atcacatggt
    10021 cctgctggag ttccgcaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
    10081 aaccggtgct cgaggtaccg atgaatagct aaggtagagg ccgcaggtaa gtatcaaggt
    10141 tacaagacag gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt
    10201 gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg
    10261 tgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
    10321 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
    10381 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
    10441 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
    10501 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
    10561 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
    10621 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
    10681 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
    10741 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
    10801 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
    10861 aattcgagct cggtacgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
    10921 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
    10981 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
    11041 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcccagcttt tgttcccttt
    11101 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
    11161 gttatccgct aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
    11221 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg
    11281 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
    11341 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
    11401 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg
    11461 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct
    11521 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt
    11581 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga
    11641 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcg
    11701 gccggccgcc ggcgtcgacg cgcgcggccg cggcgcgccg ttgctggcgt ttttccatag
    11761 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    11821 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    11881 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    11941 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    12001 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    12061 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    12121 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    12181 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    12241 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    12301 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    12361 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    12421 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    12481 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    12541 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    12601 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    12661 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    12721 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    12781 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    12841 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    12901 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    12961 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    13021 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    13081 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    13141 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    13201 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    13261 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    13321 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    13381 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    13441 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    13501 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    13561 gccctttcgt ctacgcgcgc gccggcggcg cgccgtcgac ggccggccgc ggccgc
//
