LOCUS       sequence-249437-       13646 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PPI (BiFC/TriFC) assay vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13646)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 13646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-496    N-Tag GFP[2] L20 Piezo1 EBPF2
FEATURES             Location/Qualifiers
     source          1..13646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        153..532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        533..736
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     687..707
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     733..757
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          872..1004
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     regulatory      1019..1028
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(8805..9379,9380..9382,9383..9391)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8972..8989)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9199..9218
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(9392..9394,9395..9397,9398..10111)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(9437..9458)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     intron          10157..10289
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    10298..10886
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10351..10371)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10769..10780)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10913..10930)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10919..11030
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11126..11146)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11126..11144)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11165..11181)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11165..11181)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11178..11200)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     complement(11283..11302)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    11348..11403
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(11402..11421)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(11782..12370)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11862..11881)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12541..13332,13333..13401))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13164..13183
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13402..13506)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13574..13592
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcctgcctcc aggcctcaat attggccatt agccatatta ttcattggtt atatagcata
       61 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
      121 tattggctca tgtccaatat gaccgccatg ttggcattga ttattgacta gttattaata
      181 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      241 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      301 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      361 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
      421 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
      481 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      541 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      601 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      661 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
      721 ctatataagc agagctcgtt tagtgaaccg tcagatcact agaagcttta ttgcggtagt
      781 ttatcacagt taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag
      841 ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta
      901 aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc
      961 acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccaggc
     1021 caccatggaa aagagggacc acatggtgct gctcgagtac gtcacagcag ctggcatcac
     1081 cgatgccagc gggagtcctg gcggtggatc cggaggctct gggagctctg ccagcggtgg
     1141 cagcacctcc gagccgcacg tgctgggcgc cgggctctac tggctgttgc tgccctgcac
     1201 gctcctggcg gcctccctgt tacgcttcaa tgctctctcg ctggtctatt tgctgtttct
     1261 actgctgctg ccctggcttc caggcccctc aagacacagc ataccaggtc acacaggtcg
     1321 cctgctccgt gcactactct gcctcagcct cctcttcctg gtggcccacc ttgcctttca
     1381 gatatgccta cacaccgtgc ctcacctgga ccagtttctg ggacaaaacg gtagcctttg
     1441 ggtgaaggtg tctcaacaca taggggttac gaggctggac ctgaaggaca tctttaacac
     1501 caccaggctg gtagcacctg acctgggagt gctgctggcg tcctcccttt gccttggcct
     1561 ctgtggacgc ctcacgagga aagccgggca gagtcggcgc acccaggagc tgcaggatga
     1621 tgacgatgac gatgatgacg acgatgaaga catagatgct gccccagccg tggggctgaa
     1681 gggagcccct gccctggcaa ccaaacgcag gctgtggctg gcctcccgct tccgggtcac
     1741 ggcccactgg ctgctgatga cctctggacg gacgctggtc attgtgttgc tggccctggc
     1801 aggcatagcc cacccttcgg ccttctccag catctacctg gtggtgttcc tggccatctg
     1861 cacctggtgg tcctgtcact ttcctctcag ccccctgggc tttaacaccc tctgtgtcat
     1921 ggtgagctgt tttggtgccg gccatctcat ttgcctatac tgctatcaga caccatttat
     1981 ccaggacatg ttaccccctg ggaacatctg ggccaggcta tttggtctca agaacttcgt
     2041 agacctccct aactactcca gccccaacgc cctggtactc aacactaagc acgcctggcc
     2101 catctatgtg agtcccggaa tcctgctgct gctatattac acagccacct ctctcctgaa
     2161 gctccacaag agctgtccct cagagctgag gaaggagaca cccagggagg atgaggagca
     2221 tgagctggaa ctggaccacc tggagccaga gccccaggct agggacgcca cccagggtga
     2281 gatgcccatg accacggaac ctgaccttga caactgcacc gtgcatgtac taaccagcca
     2341 gagccctgtc cgccagcgtc cagttcgccc caggctggct gagctgaaag agatgtcacc
     2401 gctacatggc ctgggccacc tcatcatgga ccagagctat gtgtgtgccc tgattgccat
     2461 gatggtgtgg agcatcatgt accacagttg gctgaccttc gtcctgctgc tctgggcctg
     2521 cctcatctgg acggtgcgta gccgtcacca gctggctatg ctctgctcgc cctgcatcct
     2581 gctgtatggg ctgacgctct gctgcctgag gtatgtgtgg gccatggaac ttcctgagct
     2641 gcccaccacc ctgggccctg tcagcctgca ccagttggga ctggaacaca cacgctaccc
     2701 ttgcctggac ctcggtgcca tgctcctcta tctgctcaca ttctggctcc ttctgcgtca
     2761 gttcgtgaag gagaagctgc tgaagaagca aaaggtgcct gcggcgctgc tggaggtcac
     2821 ggtggccgac actgagccca cacagaccca gacgctgctg cggagcctgg gggagctggt
     2881 caccggcatc tacgtcaaat actggatcta tgtgtgcgcc ggcatgttca ttgtggtcag
     2941 cttcgccggc cgcctggtgg tctacaaaat cgtctacatg ttcctcttcc tgctgtgcct
     3001 caccttgttc caggtctact acaccctgtg gaggaagctg ctacgtgtct tctggtggct
     3061 cgtggtggcc tatacaatgc tcgtgctcat cgctgtgtac accttccagt tccaggactt
     3121 ccccacctat tggcgcaacc tcacgggctt cacagacgag cagttgggcg acctgggcct
     3181 ggagcagttc agtgtgtcgg agctcttttc cagtatcctc atccctggct tcttcctgct
     3241 ggcctgcatc ctgcagctgc actacttcca cagaccgttc atgcagctca ctgacctgga
     3301 gcacgtgccg ccaccaggca cccgccaccc tcgatgggct cacaggcagg atgcagtgag
     3361 cgaggcccct ctgcttgagc atcaggagga agaggaagtc ttcagggaag atgggcagag
     3421 catggatggg ccccaccagg ccacacaggt ccctgagggt acggccagca agtggggcct
     3481 ggtggctgac cggctgctgg acctagcggc cagcttctcg gctgtcctca cccgaatcca
     3541 ggtgttcgtt cggcgcttgc tagaacttca cgtcttcaag ctggtggccc tctacactgt
     3601 ctgggtggcc ctgaaagaag tgtctgtgat gaacctgctg ctggtggtgc tatgggcctt
     3661 cgccttgccc tatccgcgct tccggcccat ggcttcctgc ctgtccaccg tgtggacctg
     3721 tatcatcatt gtatgcaaga tgctctatca gctcaagatt gtcaacccgc atgagtactc
     3781 cagcaactgc actgagccct tccccaacaa taccaacttg cagcctttgg agatcaacca
     3841 gtctttgctg taccgtggcc ctgttgaccc tgccaactgg tttggggtgc ggaagggtta
     3901 ccccaacttg ggctatatcc agaaccacct gcagatcctt ctgttgctgg tgtttgaggc
     3961 cgtggtgtac cggcgccaag agcactaccg ccggcagcac cagcaggccc ctctgcccgc
     4021 ccaggctgtg tgcgcagatg gcacccgcca gaggttggac caggacctac ttagctgcct
     4081 caagtatttc atcaacttct tcttctacaa attcgggctg gagatctgct tcttgatggc
     4141 cgtgaatgtg attgggcagc gtatgaactt catggtgatc ttacacggtt gctggttggt
     4201 ggccatcctt acacgccggc gccgtgaggc catcgcccgc ctctggccta actactgtct
     4261 gttcctcacg ctgttcctgc tgtaccagta cctgctgtgt ttgggcatgc cccctgcact
     4321 gtgcattgac tatccatggc gctggagcaa ggccatcccc atgaattccg ccctcatcaa
     4381 gtggctgtac ctacctgact tcttcagagc ccccaactcc accaacctta tcagtgactt
     4441 cctcctgctg ctttgcgcct cccagcagtg gcaggtcttc tcagcggagc gaacggagga
     4501 gtggcaacgc atggcgggca tcaacactga ccacctggag cccctgcgtg gggagcccaa
     4561 ccctataccc aacttcatcc actgcaggtc ctatctggat atgctgaagg tggccgtctt
     4621 ccgctacctg ttctggctgg tgctcgttgt ggtgtttgtt gcgggggcca cccgcataag
     4681 catcttcggg ctggggtacc tgctagcctg cttctacctg ctgctgtttg gcactaccct
     4741 gctgcagaag gacacgcgag cccagctcgt gctgtgggac tgcctcatcc tctataatgt
     4801 cactgtcatc atctctaaga atatgctgtc gctcctgtcc tgtgtcttcg tggagcaaat
     4861 gcagagcaac ttctgctggg tcatccagct cttcagcctc gtgtgcacag tcaaaggcta
     4921 ctatgatccc aaagagatga tgaccaggga ccgggactgc ctgctgcctg tggaggaggc
     4981 cgggatcatc tgggacagta tctgcttctt cttcctgctc ttgcaacggc gcatctttct
     5041 cagccactac ttcctgcatg tcagcgctga cctgaaagcc acagccctgc aggcatccag
     5101 gggctttgcc ctctacaatg cagccaacct gaagagcatc aacttccatc gccagattga
     5161 ggagaagtcc ctggcccagc tgaaaagaca gatgaagcgc atccgtgcca aacaggagaa
     5221 gtacaggcag agccaggcaa gtcgtggcca actccagtcc aaagaccctc aggatcccag
     5281 ccaggagcca gggcctgaca gcccaggggg ctcctccccg ccacggagac agtggtggcg
     5341 cccctggctg gaccacgcca cagtcatcca ctctggcgac tacttcctgt ttgagtcaga
     5401 tagcgaggag gaagaggagg ccctacctga ggaccccagg cctgcagctc agagtgcctt
     5461 ccagatggca taccaggcat gggtaaccaa tgcccagaca gtgctgaggc agcgtcggga
     5521 gcgggcacgg caggagcggg cagagcagct ggcttctgga ggtgacttga acccagatgt
     5581 ggaaccagta gatgtcccag aagatgagat ggcaggccgt agccacatga tgcagcgtgt
     5641 gctaagcacc atgcagttcc tgtgggtgct gggccaggcc acggtagacg ggctgacgcg
     5701 ctggctgcgt gcattcacga agcaccaccg caccatgagc gatgtgctgt gcgcagagcg
     5761 ctacctgctc acccaggagc ttcttcgggt tggagaggta cgccgaggtg tgctggacca
     5821 gctttatgtg ggtgaagatg aggccacatt gtcaggtccc gtggagaccc gggatggacc
     5881 cagcacagcc tcaagtgggc tgggagccga agagcctttg agtagcatga cagacgacac
     5941 cagcagcccc ctgagcacag gctataacac ccgcagtggc agtgaggaga ttgtcaccga
     6001 cgctggggac ctccaggctg ggacctccct gcacggctcc caagagcttt tagccaatgc
     6061 tcgtacccgg atgcgcacgg ccagcgagct gctactggat aggcgcctgc atatccctga
     6121 gctggaggag gccgagcggt ttgaggcaca gcagggccgg actctgcggc tgctcagggc
     6181 tgggtaccag tgcgtggcgg cacactcgga gctgctctgt tacttcatca tcatccttaa
     6241 ccacatggtg acagcctcgg ctgcctccct ggtgctgccc gtgcttgtgt tcctgtgggc
     6301 catgctgacc atcccgaggc ctagcaagcg cttttggatg acagctatcg tcttcactga
     6361 ggtcatggtg gtcaccaaat acctgttcca gttcggcttc ttcccctgga acagctacgt
     6421 tgtgctgcgg cgctatgaga acaagcccta cttccctccg cgaatcctgg gccttgagaa
     6481 aacggacagc tacatcaagt atgacctggt gcagctcatg gccctcttct tccaccgctc
     6541 gcagctactg tgttatggcc tctgggacca tgaggaggat cgctatccca aggaccattg
     6601 caggagtagt gtgaaggacc gggaggccaa ggaagagcca gaagctaagc tggaatcgca
     6661 gtctgagaca ggcactgggc atcccaagga gccagtgttg gccggtactc ccagggacca
     6721 catccaaggg aaaggaagta ttagatccaa ggatgttatc caagatcccc cagaggacct
     6781 taagccccgg cacacgaggc acatcagcat acgcttcagg aggcgcaagg agactccagg
     6841 acccaaagga acagcagtca tggagactga gcacgaggag ggagaaggaa aagaaactac
     6901 agagagaaag aggccgcgtc acactcaaga aaaatcgaag tttcgggaga gaatgaaggc
     6961 agctgggcgc cggctgcaga gcttctgtgt gtcactggcc cagagcttct accaaccctt
     7021 gcagcgtttc ttccatgaca ttctgcacac aaagtaccgg gcggccaccg acgtctacgc
     7081 cctcatgttc ctggccgata ttgtcgacat catcatcatc atctttggtt tttgggcttt
     7141 tgggaagcac tctgcagcca cagacattgc atcctcgctg tcagatgacc aggtgccaca
     7201 ggccttcctg ttcatgctgc tggtccagtt tggcaccatg gtcatcgacc gtgccctcta
     7261 cctgcgcaag actgtcctgg gaaagctggc ctttcaggtg gtcctggtgg tggcgattca
     7321 catctggatg ttctttatct taccggctgt cactgagagg atgttcagcc agaatgcggt
     7381 ggcacagctg tggtacttcg tcaagtgcat ttactttgcc ctgtccgcct accagatccg
     7441 ctgtggctac cccacccgta tcttgggcaa cttcctcacc aagaaataca accatctaaa
     7501 cctcttcctt ttccaggggt tccgtctagt gccgttcctg gtggagctgc gggccgtcat
     7561 ggactgggtg tggaccgaca ccacgctgtc cctgtccaac tggatgtgtg tggaagacat
     7621 ctatgccaac atctttatca tcaagtgcag ccgagagaca gagaagaaat acccccagcc
     7681 caaggggcag aagaagaaga aaattgtcaa gtatggtatg ggaggcctca ttatcctctt
     7741 cctcatcgcc atcatctggt tccctctgct cttcatgtca ctgatccgct ctgtggtcgg
     7801 ggtcgtcaac cagcccattg atgtcaccgt caccctcaag ctaggcggct atgagccact
     7861 gttcaccatg agcgcccagc agccatccat tgtgccattc acaccccagg cctacgagga
     7921 gctgtcccag cagtttgacc cctatccact agccatgcag ttcattagcc agtacagtcc
     7981 tgaggacatc gtcactgcac agatcgaggg cagctcgggg gcgctgtggc gcatcagccc
     8041 acccagccga gcccagatga agcaggagct gtacaacggc acagccgaca ttacactgcg
     8101 ctttacctgg aatttccaaa gggacctggc caagggtggc actgtggagt atactaatga
     8161 gaagcacacc ttggagctgg cccccaacag tacggcacga aggcagctgg cccaactgct
     8221 cgagggcaga cctgaccagt cagtggtcat tccccatctc ttccccaagt acattcgtgc
     8281 tcccaatggg cctgaagcca accctgtgaa gcagctgcag ccagatgagg aagaggacta
     8341 ccttggtgtg cgcatccagc tgcggaggga gcaagtgggc acaggggcct ctggggagca
     8401 agcgggcacc aaggcctccg acttcctcga gtggtgggtc atcgagctgc aggactgcaa
     8461 ggctgactgc aacctgctgc ccatggtcat cttcagtgac aaggtcagcc cacctagcct
     8521 gggcttcctg gccggctacg ggattgtggg gctgtatgtc tccatcgtgc tggtggttgg
     8581 caagtttgtg cggggcttct tcagcgagat ctctcactcc atcatgttcg aggaactgcc
     8641 gtgtgtggac cgcatcctca agctgtgcca ggacatcttc ttggtgcgcg agacccggga
     8701 gctggagctg gaggaggagc tatacgccaa gctcatcttc ctgtaccgat ctccagagac
     8761 catgattaag tggacacgtg agagggagta agaattcaga tccgcccctc tccctccccc
     8821 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     8881 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     8941 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     9001 aatgtcgtga aggaagcagt tcctctggta gcttcttgaa gacaaacaac gtctgtagcg
     9061 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     9121 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     9181 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     9241 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt
     9301 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     9361 tgaaaaacac gatgataata tggccacaac catggtgagc aagggcgagg agctgttcac
     9421 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     9481 gaggggcgag ggcgagggcg atgccaccaa cggcaagctg accctgaagt tcatctgcac
     9541 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgagcc acggcgtgca
     9601 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     9661 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcacct acaagacccg
     9721 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcgtcga
     9781 cttcaaggag gacggcaaca tcctggggca caagctggag tacaacttca acagccacaa
     9841 catctatatc atggccgtca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     9901 caacgtggag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     9961 cgacggcccc gtgctgctgc ccgacagcca ctacctgagc acccagtccg tgctgagcaa
    10021 agaccccaac gagaagcgcg atcacatggt cctgctggag ttccgcaccg ccgccgggat
    10081 cactctcggc atggacgagc tgtacaagta aaccggtgct cgaggtaccg atgaatagct
    10141 aaggtagagg ccgcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
    10201 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
    10261 tgacatccac tttgcctttc tctccacagg tgtcgacaat caacctctgg attacaaaat
    10321 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
    10381 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
    10441 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
    10501 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
    10561 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
    10621 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
    10681 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
    10741 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
    10801 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
    10861 gatctccctt tgggccgcct ccccgcctgg aattcgagct cggtacgatc agcctcgact
    10921 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
    10981 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
    11041 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
    11101 gaagacaata gcccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
    11161 catggtcata gctgtttcct gtgtgaaatt gttatccgct aattcactcc tcaggtgcag
    11221 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
    11281 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
    11341 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
    11401 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
    11461 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
    11521 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
    11581 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
    11641 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
    11701 atagctgtcc ctcttctctt atggagatcg gccggccgcc ggcgtcgacg cgcgcggccg
    11761 cggcgcgccg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    11821 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    11881 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    11941 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    12001 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    12061 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    12121 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    12181 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
    12241 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    12301 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    12361 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    12421 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    12481 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    12541 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    12601 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    12661 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    12721 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    12781 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    12841 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    12901 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    12961 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    13021 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    13081 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    13141 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    13201 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    13261 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    13321 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    13381 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    13441 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    13501 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
    13561 attaacctat aaaaataggc gtatcacgag gccctttcgt ctacgcgcgc gccggcggcg
    13621 cgccgtcgac ggccggccgc ggccgc
//
