LOCUS       sequence-249439-       13682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PPI (BiFC/TriFC) assay vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13682)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 13682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-498    N-Tag GFP[2] L32 Piezo1 EBPF2
FEATURES             Location/Qualifiers
     source          1..13682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        153..532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        533..736
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     687..707
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     733..757
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          872..1004
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     regulatory      1019..1028
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(8841..9415,9416..9418,9419..9427)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9008..9025)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9235..9254
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(9428..9430,9431..9433,9434..10147)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(9473..9494)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     intron          10193..10325
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    10334..10922
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10387..10407)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10805..10816)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10949..10966)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10955..11066
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11162..11182)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11162..11180)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11201..11217)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11201..11217)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11214..11236)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     complement(11319..11338)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    11384..11439
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(11438..11457)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(11818..12406)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11898..11917)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12577..13368,13369..13437))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13200..13219
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13438..13542)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13610..13628
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcctgcctcc aggcctcaat attggccatt agccatatta ttcattggtt atatagcata
       61 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
      121 tattggctca tgtccaatat gaccgccatg ttggcattga ttattgacta gttattaata
      181 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      241 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      301 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      361 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
      421 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
      481 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      541 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      601 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      661 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
      721 ctatataagc agagctcgtt tagtgaaccg tcagatcact agaagcttta ttgcggtagt
      781 ttatcacagt taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag
      841 ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta
      901 aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc
      961 acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccaggc
     1021 caccatggaa aagagggacc acatggtgct gctcgagtac gtcacagcag ctggcatcac
     1081 cgatgccagc gacgtgggtg gcggagggag cgaaggcggc ggatccggag ggcctggctc
     1141 tggcggagag gggagcgctg gaggcgggtc agccggtggt gggagtgagc cgcacgtgct
     1201 gggcgccggg ctctactggc tgttgctgcc ctgcacgctc ctggcggcct ccctgttacg
     1261 cttcaatgct ctctcgctgg tctatttgct gtttctactg ctgctgccct ggcttccagg
     1321 cccctcaaga cacagcatac caggtcacac aggtcgcctg ctccgtgcac tactctgcct
     1381 cagcctcctc ttcctggtgg cccaccttgc ctttcagata tgcctacaca ccgtgcctca
     1441 cctggaccag tttctgggac aaaacggtag cctttgggtg aaggtgtctc aacacatagg
     1501 ggttacgagg ctggacctga aggacatctt taacaccacc aggctggtag cacctgacct
     1561 gggagtgctg ctggcgtcct ccctttgcct tggcctctgt ggacgcctca cgaggaaagc
     1621 cgggcagagt cggcgcaccc aggagctgca ggatgatgac gatgacgatg atgacgacga
     1681 tgaagacata gatgctgccc cagccgtggg gctgaaggga gcccctgccc tggcaaccaa
     1741 acgcaggctg tggctggcct cccgcttccg ggtcacggcc cactggctgc tgatgacctc
     1801 tggacggacg ctggtcattg tgttgctggc cctggcaggc atagcccacc cttcggcctt
     1861 ctccagcatc tacctggtgg tgttcctggc catctgcacc tggtggtcct gtcactttcc
     1921 tctcagcccc ctgggcttta acaccctctg tgtcatggtg agctgttttg gtgccggcca
     1981 tctcatttgc ctatactgct atcagacacc atttatccag gacatgttac cccctgggaa
     2041 catctgggcc aggctatttg gtctcaagaa cttcgtagac ctccctaact actccagccc
     2101 caacgccctg gtactcaaca ctaagcacgc ctggcccatc tatgtgagtc ccggaatcct
     2161 gctgctgcta tattacacag ccacctctct cctgaagctc cacaagagct gtccctcaga
     2221 gctgaggaag gagacaccca gggaggatga ggagcatgag ctggaactgg accacctgga
     2281 gccagagccc caggctaggg acgccaccca gggtgagatg cccatgacca cggaacctga
     2341 ccttgacaac tgcaccgtgc atgtactaac cagccagagc cctgtccgcc agcgtccagt
     2401 tcgccccagg ctggctgagc tgaaagagat gtcaccgcta catggcctgg gccacctcat
     2461 catggaccag agctatgtgt gtgccctgat tgccatgatg gtgtggagca tcatgtacca
     2521 cagttggctg accttcgtcc tgctgctctg ggcctgcctc atctggacgg tgcgtagccg
     2581 tcaccagctg gctatgctct gctcgccctg catcctgctg tatgggctga cgctctgctg
     2641 cctgaggtat gtgtgggcca tggaacttcc tgagctgccc accaccctgg gccctgtcag
     2701 cctgcaccag ttgggactgg aacacacacg ctacccttgc ctggacctcg gtgccatgct
     2761 cctctatctg ctcacattct ggctccttct gcgtcagttc gtgaaggaga agctgctgaa
     2821 gaagcaaaag gtgcctgcgg cgctgctgga ggtcacggtg gccgacactg agcccacaca
     2881 gacccagacg ctgctgcgga gcctggggga gctggtcacc ggcatctacg tcaaatactg
     2941 gatctatgtg tgcgccggca tgttcattgt ggtcagcttc gccggccgcc tggtggtcta
     3001 caaaatcgtc tacatgttcc tcttcctgct gtgcctcacc ttgttccagg tctactacac
     3061 cctgtggagg aagctgctac gtgtcttctg gtggctcgtg gtggcctata caatgctcgt
     3121 gctcatcgct gtgtacacct tccagttcca ggacttcccc acctattggc gcaacctcac
     3181 gggcttcaca gacgagcagt tgggcgacct gggcctggag cagttcagtg tgtcggagct
     3241 cttttccagt atcctcatcc ctggcttctt cctgctggcc tgcatcctgc agctgcacta
     3301 cttccacaga ccgttcatgc agctcactga cctggagcac gtgccgccac caggcacccg
     3361 ccaccctcga tgggctcaca ggcaggatgc agtgagcgag gcccctctgc ttgagcatca
     3421 ggaggaagag gaagtcttca gggaagatgg gcagagcatg gatgggcccc accaggccac
     3481 acaggtccct gagggtacgg ccagcaagtg gggcctggtg gctgaccggc tgctggacct
     3541 agcggccagc ttctcggctg tcctcacccg aatccaggtg ttcgttcggc gcttgctaga
     3601 acttcacgtc ttcaagctgg tggccctcta cactgtctgg gtggccctga aagaagtgtc
     3661 tgtgatgaac ctgctgctgg tggtgctatg ggccttcgcc ttgccctatc cgcgcttccg
     3721 gcccatggct tcctgcctgt ccaccgtgtg gacctgtatc atcattgtat gcaagatgct
     3781 ctatcagctc aagattgtca acccgcatga gtactccagc aactgcactg agcccttccc
     3841 caacaatacc aacttgcagc ctttggagat caaccagtct ttgctgtacc gtggccctgt
     3901 tgaccctgcc aactggtttg gggtgcggaa gggttacccc aacttgggct atatccagaa
     3961 ccacctgcag atccttctgt tgctggtgtt tgaggccgtg gtgtaccggc gccaagagca
     4021 ctaccgccgg cagcaccagc aggcccctct gcccgcccag gctgtgtgcg cagatggcac
     4081 ccgccagagg ttggaccagg acctacttag ctgcctcaag tatttcatca acttcttctt
     4141 ctacaaattc gggctggaga tctgcttctt gatggccgtg aatgtgattg ggcagcgtat
     4201 gaacttcatg gtgatcttac acggttgctg gttggtggcc atccttacac gccggcgccg
     4261 tgaggccatc gcccgcctct ggcctaacta ctgtctgttc ctcacgctgt tcctgctgta
     4321 ccagtacctg ctgtgtttgg gcatgccccc tgcactgtgc attgactatc catggcgctg
     4381 gagcaaggcc atccccatga attccgccct catcaagtgg ctgtacctac ctgacttctt
     4441 cagagccccc aactccacca accttatcag tgacttcctc ctgctgcttt gcgcctccca
     4501 gcagtggcag gtcttctcag cggagcgaac ggaggagtgg caacgcatgg cgggcatcaa
     4561 cactgaccac ctggagcccc tgcgtgggga gcccaaccct atacccaact tcatccactg
     4621 caggtcctat ctggatatgc tgaaggtggc cgtcttccgc tacctgttct ggctggtgct
     4681 cgttgtggtg tttgttgcgg gggccacccg cataagcatc ttcgggctgg ggtacctgct
     4741 agcctgcttc tacctgctgc tgtttggcac taccctgctg cagaaggaca cgcgagccca
     4801 gctcgtgctg tgggactgcc tcatcctcta taatgtcact gtcatcatct ctaagaatat
     4861 gctgtcgctc ctgtcctgtg tcttcgtgga gcaaatgcag agcaacttct gctgggtcat
     4921 ccagctcttc agcctcgtgt gcacagtcaa aggctactat gatcccaaag agatgatgac
     4981 cagggaccgg gactgcctgc tgcctgtgga ggaggccggg atcatctggg acagtatctg
     5041 cttcttcttc ctgctcttgc aacggcgcat ctttctcagc cactacttcc tgcatgtcag
     5101 cgctgacctg aaagccacag ccctgcaggc atccaggggc tttgccctct acaatgcagc
     5161 caacctgaag agcatcaact tccatcgcca gattgaggag aagtccctgg cccagctgaa
     5221 aagacagatg aagcgcatcc gtgccaaaca ggagaagtac aggcagagcc aggcaagtcg
     5281 tggccaactc cagtccaaag accctcagga tcccagccag gagccagggc ctgacagccc
     5341 agggggctcc tccccgccac ggagacagtg gtggcgcccc tggctggacc acgccacagt
     5401 catccactct ggcgactact tcctgtttga gtcagatagc gaggaggaag aggaggccct
     5461 acctgaggac cccaggcctg cagctcagag tgccttccag atggcatacc aggcatgggt
     5521 aaccaatgcc cagacagtgc tgaggcagcg tcgggagcgg gcacggcagg agcgggcaga
     5581 gcagctggct tctggaggtg acttgaaccc agatgtggaa ccagtagatg tcccagaaga
     5641 tgagatggca ggccgtagcc acatgatgca gcgtgtgcta agcaccatgc agttcctgtg
     5701 ggtgctgggc caggccacgg tagacgggct gacgcgctgg ctgcgtgcat tcacgaagca
     5761 ccaccgcacc atgagcgatg tgctgtgcgc agagcgctac ctgctcaccc aggagcttct
     5821 tcgggttgga gaggtacgcc gaggtgtgct ggaccagctt tatgtgggtg aagatgaggc
     5881 cacattgtca ggtcccgtgg agacccggga tggacccagc acagcctcaa gtgggctggg
     5941 agccgaagag cctttgagta gcatgacaga cgacaccagc agccccctga gcacaggcta
     6001 taacacccgc agtggcagtg aggagattgt caccgacgct ggggacctcc aggctgggac
     6061 ctccctgcac ggctcccaag agcttttagc caatgctcgt acccggatgc gcacggccag
     6121 cgagctgcta ctggataggc gcctgcatat ccctgagctg gaggaggccg agcggtttga
     6181 ggcacagcag ggccggactc tgcggctgct cagggctggg taccagtgcg tggcggcaca
     6241 ctcggagctg ctctgttact tcatcatcat ccttaaccac atggtgacag cctcggctgc
     6301 ctccctggtg ctgcccgtgc ttgtgttcct gtgggccatg ctgaccatcc cgaggcctag
     6361 caagcgcttt tggatgacag ctatcgtctt cactgaggtc atggtggtca ccaaatacct
     6421 gttccagttc ggcttcttcc cctggaacag ctacgttgtg ctgcggcgct atgagaacaa
     6481 gccctacttc cctccgcgaa tcctgggcct tgagaaaacg gacagctaca tcaagtatga
     6541 cctggtgcag ctcatggccc tcttcttcca ccgctcgcag ctactgtgtt atggcctctg
     6601 ggaccatgag gaggatcgct atcccaagga ccattgcagg agtagtgtga aggaccggga
     6661 ggccaaggaa gagccagaag ctaagctgga atcgcagtct gagacaggca ctgggcatcc
     6721 caaggagcca gtgttggccg gtactcccag ggaccacatc caagggaaag gaagtattag
     6781 atccaaggat gttatccaag atcccccaga ggaccttaag ccccggcaca cgaggcacat
     6841 cagcatacgc ttcaggaggc gcaaggagac tccaggaccc aaaggaacag cagtcatgga
     6901 gactgagcac gaggagggag aaggaaaaga aactacagag agaaagaggc cgcgtcacac
     6961 tcaagaaaaa tcgaagtttc gggagagaat gaaggcagct gggcgccggc tgcagagctt
     7021 ctgtgtgtca ctggcccaga gcttctacca acccttgcag cgtttcttcc atgacattct
     7081 gcacacaaag taccgggcgg ccaccgacgt ctacgccctc atgttcctgg ccgatattgt
     7141 cgacatcatc atcatcatct ttggtttttg ggcttttggg aagcactctg cagccacaga
     7201 cattgcatcc tcgctgtcag atgaccaggt gccacaggcc ttcctgttca tgctgctggt
     7261 ccagtttggc accatggtca tcgaccgtgc cctctacctg cgcaagactg tcctgggaaa
     7321 gctggccttt caggtggtcc tggtggtggc gattcacatc tggatgttct ttatcttacc
     7381 ggctgtcact gagaggatgt tcagccagaa tgcggtggca cagctgtggt acttcgtcaa
     7441 gtgcatttac tttgccctgt ccgcctacca gatccgctgt ggctacccca cccgtatctt
     7501 gggcaacttc ctcaccaaga aatacaacca tctaaacctc ttccttttcc aggggttccg
     7561 tctagtgccg ttcctggtgg agctgcgggc cgtcatggac tgggtgtgga ccgacaccac
     7621 gctgtccctg tccaactgga tgtgtgtgga agacatctat gccaacatct ttatcatcaa
     7681 gtgcagccga gagacagaga agaaataccc ccagcccaag gggcagaaga agaagaaaat
     7741 tgtcaagtat ggtatgggag gcctcattat cctcttcctc atcgccatca tctggttccc
     7801 tctgctcttc atgtcactga tccgctctgt ggtcggggtc gtcaaccagc ccattgatgt
     7861 caccgtcacc ctcaagctag gcggctatga gccactgttc accatgagcg cccagcagcc
     7921 atccattgtg ccattcacac cccaggccta cgaggagctg tcccagcagt ttgaccccta
     7981 tccactagcc atgcagttca ttagccagta cagtcctgag gacatcgtca ctgcacagat
     8041 cgagggcagc tcgggggcgc tgtggcgcat cagcccaccc agccgagccc agatgaagca
     8101 ggagctgtac aacggcacag ccgacattac actgcgcttt acctggaatt tccaaaggga
     8161 cctggccaag ggtggcactg tggagtatac taatgagaag cacaccttgg agctggcccc
     8221 caacagtacg gcacgaaggc agctggccca actgctcgag ggcagacctg accagtcagt
     8281 ggtcattccc catctcttcc ccaagtacat tcgtgctccc aatgggcctg aagccaaccc
     8341 tgtgaagcag ctgcagccag atgaggaaga ggactacctt ggtgtgcgca tccagctgcg
     8401 gagggagcaa gtgggcacag gggcctctgg ggagcaagcg ggcaccaagg cctccgactt
     8461 cctcgagtgg tgggtcatcg agctgcagga ctgcaaggct gactgcaacc tgctgcccat
     8521 ggtcatcttc agtgacaagg tcagcccacc tagcctgggc ttcctggccg gctacgggat
     8581 tgtggggctg tatgtctcca tcgtgctggt ggttggcaag tttgtgcggg gcttcttcag
     8641 cgagatctct cactccatca tgttcgagga actgccgtgt gtggaccgca tcctcaagct
     8701 gtgccaggac atcttcttgg tgcgcgagac ccgggagctg gagctggagg aggagctata
     8761 cgccaagctc atcttcctgt accgatctcc agagaccatg attaagtgga cacgtgagag
     8821 ggagtaagaa ttcagatccg cccctctccc tccccccccc ctaacgttac tggccgaagc
     8881 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     8941 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     9001 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     9061 ctggtagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     9121 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     9181 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     9241 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     9301 tctgatctgg ggcctcggta cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     9361 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
     9421 cacaaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     9481 gctggacggc gacgtaaacg gccacaagtt cagcgtgagg ggcgagggcg agggcgatgc
     9541 caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     9601 gcccaccctc gtgaccaccc tgagccacgg cgtgcagtgc ttcgcccgct accccgacca
     9661 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     9721 catcttcttc aaggacgacg gcacctacaa gacccgcgcc gaggtgaagt tcgagggcga
     9781 caccctggtg aaccgcatcg agctgaaggg cgtcgacttc aaggaggacg gcaacatcct
     9841 ggggcacaag ctggagtaca acttcaacag ccacaacatc tatatcatgg ccgtcaagca
     9901 gaagaacggc atcaaggtga acttcaagat ccgccacaac gtggaggacg gcagcgtgca
     9961 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
    10021 cagccactac ctgagcaccc agtccgtgct gagcaaagac cccaacgaga agcgcgatca
    10081 catggtcctg ctggagttcc gcaccgccgc cgggatcact ctcggcatgg acgagctgta
    10141 caagtaaacc ggtgctcgag gtaccgatga atagctaagg tcgaggccgc aggtaagtat
    10201 caaggttaca agacaggttt aaggagacca atagaaactg ggcttgtcga gacagagaag
    10261 actcttgcgt ttctgatagg cacctattgg tcttactgac atccactttg cctttctctc
    10321 cacaggtgtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
    10381 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
    10441 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
    10501 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
    10561 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
    10621 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
    10681 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
    10741 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
    10801 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
    10861 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
    10921 gcctggaatt cgagctcggt acgatcagcc tcgactgtgc cttctagttg ccagccatct
    10981 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
    11041 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
    11101 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagccc agcttttgtt
    11161 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
    11221 gaaattgtta tccgctaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct
    11281 ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat
    11341 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
    11401 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
    11461 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
    11521 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
    11581 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
    11641 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
    11701 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
    11761 agatcggccg gccgccggcg tcgacgcgcg cggccgcggc gcgccgttgc tggcgttttt
    11821 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    11881 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    11941 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    12001 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
    12061 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    12121 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    12181 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    12241 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    12301 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    12361 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    12421 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    12481 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    12541 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    12601 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    12661 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    12721 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    12781 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    12841 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    12901 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    12961 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    13021 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    13081 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    13141 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    13201 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    13261 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    13321 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    13381 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    13441 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    13501 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    13561 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    13621 cacgaggccc tttcgtctac gcgcgcgccg gcggcgcgcc gtcgacggcc ggccgcggcc
    13681 gc
//
