LOCUS       sequence-249441-       13601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PPI (BiFC/TriFC) assay vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13601)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 13601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-500    N-Tag GFP[1] L10 Piezo1 mCherry
FEATURES             Location/Qualifiers
     source          1..13601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        153..532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        533..736
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     687..707
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     733..757
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          872..1004
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     regulatory      1019..1028
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(8769..9343,9344..9346,9347..9355)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8936..8953)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9163..9182
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9356..10066
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9501..9519)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9769..9788
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     intron          10112..10244
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    10253..10841
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10306..10326)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10724..10735)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10868..10885)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10874..10985
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11081..11101)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11081..11099)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11120..11136)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11120..11136)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11133..11155)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     complement(11238..11257)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    11303..11358
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(11357..11376)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(11737..12325)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11817..11836)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12496..13287,13288..13356))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13119..13138
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13357..13461)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13529..13547
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcctgcctcc aggcctcaat attggccatt agccatatta ttcattggtt atatagcata
       61 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
      121 tattggctca tgtccaatat gaccgccatg ttggcattga ttattgacta gttattaata
      181 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      241 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      301 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      361 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
      421 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
      481 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      541 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      601 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      661 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
      721 ctatataagc agagctcgtt tagtgaaccg tcagatcact agaagcttta ttgcggtagt
      781 ttatcacagt taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag
      841 ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta
      901 aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc
      961 acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccaggc
     1021 caccatggat ctgcctgatg accactacct gagcacacag accatcctct ccaaggacct
     1081 taacgggagc tctgccagcg gtggcagcac ctccgagccg cacgtgctgg gcgccgggct
     1141 ctactggctg ttgctgccct gcacgctcct ggcggcctcc ctgttacgct tcaatgctct
     1201 ctcgctggtc tatttgctgt ttctactgct gctgccctgg cttccaggcc cctcaagaca
     1261 cagcatacca ggtcacacag gtcgcctgct ccgtgcacta ctctgcctca gcctcctctt
     1321 cctggtggcc caccttgcct ttcagatatg cctacacacc gtgcctcacc tggaccagtt
     1381 tctgggacaa aacggtagcc tttgggtgaa ggtgtctcaa cacatagggg ttacgaggct
     1441 ggacctgaag gacatcttta acaccaccag gctggtagca cctgacctgg gagtgctgct
     1501 ggcgtcctcc ctttgccttg gcctctgtgg acgcctcacg aggaaagccg ggcagagtcg
     1561 gcgcacccag gagctgcagg atgatgacga tgacgatgat gacgacgatg aagacataga
     1621 tgctgcccca gccgtggggc tgaagggagc ccctgccctg gcaaccaaac gcaggctgtg
     1681 gctggcctcc cgcttccggg tcacggccca ctggctgctg atgacctctg gacggacgct
     1741 ggtcattgtg ttgctggccc tggcaggcat agcccaccct tcggccttct ccagcatcta
     1801 cctggtggtg ttcctggcca tctgcacctg gtggtcctgt cactttcctc tcagccccct
     1861 gggctttaac accctctgtg tcatggtgag ctgttttggt gccggccatc tcatttgcct
     1921 atactgctat cagacaccat ttatccagga catgttaccc cctgggaaca tctgggccag
     1981 gctatttggt ctcaagaact tcgtagacct ccctaactac tccagcccca acgccctggt
     2041 actcaacact aagcacgcct ggcccatcta tgtgagtccc ggaatcctgc tgctgctata
     2101 ttacacagcc acctctctcc tgaagctcca caagagctgt ccctcagagc tgaggaagga
     2161 gacacccagg gaggatgagg agcatgagct ggaactggac cacctggagc cagagcccca
     2221 ggctagggac gccacccagg gtgagatgcc catgaccacg gaacctgacc ttgacaactg
     2281 caccgtgcat gtactaacca gccagagccc tgtccgccag cgtccagttc gccccaggct
     2341 ggctgagctg aaagagatgt caccgctaca tggcctgggc cacctcatca tggaccagag
     2401 ctatgtgtgt gccctgattg ccatgatggt gtggagcatc atgtaccaca gttggctgac
     2461 cttcgtcctg ctgctctggg cctgcctcat ctggacggtg cgtagccgtc accagctggc
     2521 tatgctctgc tcgccctgca tcctgctgta tgggctgacg ctctgctgcc tgaggtatgt
     2581 gtgggccatg gaacttcctg agctgcccac caccctgggc cctgtcagcc tgcaccagtt
     2641 gggactggaa cacacacgct acccttgcct ggacctcggt gccatgctcc tctatctgct
     2701 cacattctgg ctccttctgc gtcagttcgt gaaggagaag ctgctgaaga agcaaaaggt
     2761 gcctgcggcg ctgctggagg tcacggtggc cgacactgag cccacacaga cccagacgct
     2821 gctgcggagc ctgggggagc tggtcaccgg catctacgtc aaatactgga tctatgtgtg
     2881 cgccggcatg ttcattgtgg tcagcttcgc cggccgcctg gtggtctaca aaatcgtcta
     2941 catgttcctc ttcctgctgt gcctcacctt gttccaggtc tactacaccc tgtggaggaa
     3001 gctgctacgt gtcttctggt ggctcgtggt ggcctataca atgctcgtgc tcatcgctgt
     3061 gtacaccttc cagttccagg acttccccac ctattggcgc aacctcacgg gcttcacaga
     3121 cgagcagttg ggcgacctgg gcctggagca gttcagtgtg tcggagctct tttccagtat
     3181 cctcatccct ggcttcttcc tgctggcctg catcctgcag ctgcactact tccacagacc
     3241 gttcatgcag ctcactgacc tggagcacgt gccgccacca ggcacccgcc accctcgatg
     3301 ggctcacagg caggatgcag tgagcgaggc ccctctgctt gagcatcagg aggaagagga
     3361 agtcttcagg gaagatgggc agagcatgga tgggccccac caggccacac aggtccctga
     3421 gggtacggcc agcaagtggg gcctggtggc tgaccggctg ctggacctag cggccagctt
     3481 ctcggctgtc ctcacccgaa tccaggtgtt cgttcggcgc ttgctagaac ttcacgtctt
     3541 caagctggtg gccctctaca ctgtctgggt ggccctgaaa gaagtgtctg tgatgaacct
     3601 gctgctggtg gtgctatggg ccttcgcctt gccctatccg cgcttccggc ccatggcttc
     3661 ctgcctgtcc accgtgtgga cctgtatcat cattgtatgc aagatgctct atcagctcaa
     3721 gattgtcaac ccgcatgagt actccagcaa ctgcactgag cccttcccca acaataccaa
     3781 cttgcagcct ttggagatca accagtcttt gctgtaccgt ggccctgttg accctgccaa
     3841 ctggtttggg gtgcggaagg gttaccccaa cttgggctat atccagaacc acctgcagat
     3901 ccttctgttg ctggtgtttg aggccgtggt gtaccggcgc caagagcact accgccggca
     3961 gcaccagcag gcccctctgc ccgcccaggc tgtgtgcgca gatggcaccc gccagaggtt
     4021 ggaccaggac ctacttagct gcctcaagta tttcatcaac ttcttcttct acaaattcgg
     4081 gctggagatc tgcttcttga tggccgtgaa tgtgattggg cagcgtatga acttcatggt
     4141 gatcttacac ggttgctggt tggtggccat ccttacacgc cggcgccgtg aggccatcgc
     4201 ccgcctctgg cctaactact gtctgttcct cacgctgttc ctgctgtacc agtacctgct
     4261 gtgtttgggc atgccccctg cactgtgcat tgactatcca tggcgctgga gcaaggccat
     4321 ccccatgaat tccgccctca tcaagtggct gtacctacct gacttcttca gagcccccaa
     4381 ctccaccaac cttatcagtg acttcctcct gctgctttgc gcctcccagc agtggcaggt
     4441 cttctcagcg gagcgaacgg aggagtggca acgcatggcg ggcatcaaca ctgaccacct
     4501 ggagcccctg cgtggggagc ccaaccctat acccaacttc atccactgca ggtcctatct
     4561 ggatatgctg aaggtggccg tcttccgcta cctgttctgg ctggtgctcg ttgtggtgtt
     4621 tgttgcgggg gccacccgca taagcatctt cgggctgggg tacctgctag cctgcttcta
     4681 cctgctgctg tttggcacta ccctgctgca gaaggacacg cgagcccagc tcgtgctgtg
     4741 ggactgcctc atcctctata atgtcactgt catcatctct aagaatatgc tgtcgctcct
     4801 gtcctgtgtc ttcgtggagc aaatgcagag caacttctgc tgggtcatcc agctcttcag
     4861 cctcgtgtgc acagtcaaag gctactatga tcccaaagag atgatgacca gggaccggga
     4921 ctgcctgctg cctgtggagg aggccgggat catctgggac agtatctgct tcttcttcct
     4981 gctcttgcaa cggcgcatct ttctcagcca ctacttcctg catgtcagcg ctgacctgaa
     5041 agccacagcc ctgcaggcat ccaggggctt tgccctctac aatgcagcca acctgaagag
     5101 catcaacttc catcgccaga ttgaggagaa gtccctggcc cagctgaaaa gacagatgaa
     5161 gcgcatccgt gccaaacagg agaagtacag gcagagccag gcaagtcgtg gccaactcca
     5221 gtccaaagac cctcaggatc ccagccagga gccagggcct gacagcccag ggggctcctc
     5281 cccgccacgg agacagtggt ggcgcccctg gctggaccac gccacagtca tccactctgg
     5341 cgactacttc ctgtttgagt cagatagcga ggaggaagag gaggccctac ctgaggaccc
     5401 caggcctgca gctcagagtg ccttccagat ggcataccag gcatgggtaa ccaatgccca
     5461 gacagtgctg aggcagcgtc gggagcgggc acggcaggag cgggcagagc agctggcttc
     5521 tggaggtgac ttgaacccag atgtggaacc agtagatgtc ccagaagatg agatggcagg
     5581 ccgtagccac atgatgcagc gtgtgctaag caccatgcag ttcctgtggg tgctgggcca
     5641 ggccacggta gacgggctga cgcgctggct gcgtgcattc acgaagcacc accgcaccat
     5701 gagcgatgtg ctgtgcgcag agcgctacct gctcacccag gagcttcttc gggttggaga
     5761 ggtacgccga ggtgtgctgg accagcttta tgtgggtgaa gatgaggcca cattgtcagg
     5821 tcccgtggag acccgggatg gacccagcac agcctcaagt gggctgggag ccgaagagcc
     5881 tttgagtagc atgacagacg acaccagcag ccccctgagc acaggctata acacccgcag
     5941 tggcagtgag gagattgtca ccgacgctgg ggacctccag gctgggacct ccctgcacgg
     6001 ctcccaagag cttttagcca atgctcgtac ccggatgcgc acggccagcg agctgctact
     6061 ggataggcgc ctgcatatcc ctgagctgga ggaggccgag cggtttgagg cacagcaggg
     6121 ccggactctg cggctgctca gggctgggta ccagtgcgtg gcggcacact cggagctgct
     6181 ctgttacttc atcatcatcc ttaaccacat ggtgacagcc tcggctgcct ccctggtgct
     6241 gcccgtgctt gtgttcctgt gggccatgct gaccatcccg aggcctagca agcgcttttg
     6301 gatgacagct atcgtcttca ctgaggtcat ggtggtcacc aaatacctgt tccagttcgg
     6361 cttcttcccc tggaacagct acgttgtgct gcggcgctat gagaacaagc cctacttccc
     6421 tccgcgaatc ctgggccttg agaaaacgga cagctacatc aagtatgacc tggtgcagct
     6481 catggccctc ttcttccacc gctcgcagct actgtgttat ggcctctggg accatgagga
     6541 ggatcgctat cccaaggacc attgcaggag tagtgtgaag gaccgggagg ccaaggaaga
     6601 gccagaagct aagctggaat cgcagtctga gacaggcact gggcatccca aggagccagt
     6661 gttggccggt actcccaggg accacatcca agggaaagga agtattagat ccaaggatgt
     6721 tatccaagat cccccagagg accttaagcc ccggcacacg aggcacatca gcatacgctt
     6781 caggaggcgc aaggagactc caggacccaa aggaacagca gtcatggaga ctgagcacga
     6841 ggagggagaa ggaaaagaaa ctacagagag aaagaggccg cgtcacactc aagaaaaatc
     6901 gaagtttcgg gagagaatga aggcagctgg gcgccggctg cagagcttct gtgtgtcact
     6961 ggcccagagc ttctaccaac ccttgcagcg tttcttccat gacattctgc acacaaagta
     7021 ccgggcggcc accgacgtct acgccctcat gttcctggcc gatattgtcg acatcatcat
     7081 catcatcttt ggtttttggg cttttgggaa gcactctgca gccacagaca ttgcatcctc
     7141 gctgtcagat gaccaggtgc cacaggcctt cctgttcatg ctgctggtcc agtttggcac
     7201 catggtcatc gaccgtgccc tctacctgcg caagactgtc ctgggaaagc tggcctttca
     7261 ggtggtcctg gtggtggcga ttcacatctg gatgttcttt atcttaccgg ctgtcactga
     7321 gaggatgttc agccagaatg cggtggcaca gctgtggtac ttcgtcaagt gcatttactt
     7381 tgccctgtcc gcctaccaga tccgctgtgg ctaccccacc cgtatcttgg gcaacttcct
     7441 caccaagaaa tacaaccatc taaacctctt ccttttccag gggttccgtc tagtgccgtt
     7501 cctggtggag ctgcgggccg tcatggactg ggtgtggacc gacaccacgc tgtccctgtc
     7561 caactggatg tgtgtggaag acatctatgc caacatcttt atcatcaagt gcagccgaga
     7621 gacagagaag aaataccccc agcccaaggg gcagaagaag aagaaaattg tcaagtatgg
     7681 tatgggaggc ctcattatcc tcttcctcat cgccatcatc tggttccctc tgctcttcat
     7741 gtcactgatc cgctctgtgg tcggggtcgt caaccagccc attgatgtca ccgtcaccct
     7801 caagctaggc ggctatgagc cactgttcac catgagcgcc cagcagccat ccattgtgcc
     7861 attcacaccc caggcctacg aggagctgtc ccagcagttt gacccctatc cactagccat
     7921 gcagttcatt agccagtaca gtcctgagga catcgtcact gcacagatcg agggcagctc
     7981 gggggcgctg tggcgcatca gcccacccag ccgagcccag atgaagcagg agctgtacaa
     8041 cggcacagcc gacattacac tgcgctttac ctggaatttc caaagggacc tggccaaggg
     8101 tggcactgtg gagtatacta atgagaagca caccttggag ctggccccca acagtacggc
     8161 acgaaggcag ctggcccaac tgctcgaggg cagacctgac cagtcagtgg tcattcccca
     8221 tctcttcccc aagtacattc gtgctcccaa tgggcctgaa gccaaccctg tgaagcagct
     8281 gcagccagat gaggaagagg actaccttgg tgtgcgcatc cagctgcgga gggagcaagt
     8341 gggcacaggg gcctctgggg agcaagcggg caccaaggcc tccgacttcc tcgagtggtg
     8401 ggtcatcgag ctgcaggact gcaaggctga ctgcaacctg ctgcccatgg tcatcttcag
     8461 tgacaaggtc agcccaccta gcctgggctt cctggccggc tacgggattg tggggctgta
     8521 tgtctccatc gtgctggtgg ttggcaagtt tgtgcggggc ttcttcagcg agatctctca
     8581 ctccatcatg ttcgaggaac tgccgtgtgt ggaccgcatc ctcaagctgt gccaggacat
     8641 cttcttggtg cgcgagaccc gggagctgga gctggaggag gagctatacg ccaagctcat
     8701 cttcctgtac cgatctccag agaccatgat taagtggaca cgtgagaggg agtaagaatt
     8761 cagatccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     8821 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     8881 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     8941 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggtagcttct
     9001 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     9061 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     9121 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     9181 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     9241 cctcggtaca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     9301 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt
     9361 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     9421 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     9481 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     9541 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     9601 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     9661 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     9721 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     9781 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     9841 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     9901 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     9961 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
    10021 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaaaccg gtgctcgagg
    10081 taccgatgaa tagctaaggt cgaggccgca ggtaagtatc aaggttacaa gacaggttta
    10141 aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc
    10201 acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcg acaatcaacc
    10261 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
    10321 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
    10381 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
    10441 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
    10501 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
    10561 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
    10621 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
    10681 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
    10741 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
    10801 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta
    10861 cgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
    10921 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
    10981 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
    11041 aagggggagg attgggaaga caatagccca gcttttgttc cctttagtga gggttaattg
    11101 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctaattc
    11161 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct
    11221 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc
    11281 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
    11341 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
    11401 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa
    11461 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
    11521 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
    11581 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
    11641 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatcggccgg ccgccggcgt
    11701 cgacgcgcgc ggccgcggcg cgccgttgct ggcgtttttc cataggctcc gcccccctga
    11761 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    11821 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    11881 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    11941 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    12001 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    12061 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    12121 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    12181 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    12241 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    12301 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    12361 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    12421 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    12481 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    12541 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    12601 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    12661 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    12721 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    12781 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    12841 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    12901 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    12961 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    13021 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    13081 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    13141 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    13201 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    13261 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    13321 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    13381 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    13441 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    13501 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctacg
    13561 cgcgcgccgg cggcgcgccg tcgacggccg gccgcggccg c
//
