LOCUS       sequence-249627-        5356 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For in vitro translation of WT ELL2 with HA tag inserted near 
            N-term.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Martincic K, Alkan SA, Cheatle A, Borghesi L, Milcarek C
  TITLE     Transcription elongation factor ELL2 directs immunoglobulin 
            secretion in plasma cells by stimulating altered RNA processing.
  JOURNAL   Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009
            Sep 13.
  PUBMED    19749764
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.1786"; 
            journalName: "Nat Immunol"; date: "2009-10"; volume: "10"; issue: 
            "10"; pages: "1102-9"
COMMENT     Sequence Label: HA tagged Xba ELL2
FEATURES             Location/Qualifiers
     source          1..5356
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(52..85)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(641..652)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1350..1376
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(2546..2564)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2585..2601)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2585..2601)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2598..2620)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2609..2625
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2633..2639,2640..2657,2658..2663))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2678..2699
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2816..2833)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2987..3575)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3067..3086)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3746..4537,4538..4606))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4369..4388
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4607..4711)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4737..5192)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4874..4895)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5086..5105
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5319..5341
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5333..5350
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5334..5350
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattggag ctccaccgcg gtggcggccg cataacttcg
       61 tatagcatac attatacgaa gttatggatc aggccaaatc ggccgagctc gaattcgtcg
      121 agagcgggat aagttggcgc gtcacctaga cgtcagcctc ttacctctcg gacgtcccgt
      181 cccaggagca aaactctagt gactttgggc ccgcccccgt gagagtggag ggcggccgat
      241 gagggcggtt ctgaagggaa caagaccaat gagacgagcc cggggcgggg cgcggggccg
      301 gagggtgggg cgggtggcgg cggtggcagt ccgaggtctg gctccgcgca gtatatgaca
      361 gtacgtcagc agcgggatgg ccctagcagt ggcggcggct gcagaagccc aagcagccgc
      421 ggccgcagtg gaggctagag ccggagcggc ggcggcggcg gcaccccggg gagtttaaga
      481 tggcggcggg ggggacaggg ggcctgcggg aggagcagcg ctatgggctg tcgtgcggac
      541 ggctggggca ggacaacatc accgtactgc atgtgaagct caccgagacg gcgatccggg
      601 cgctcgagac ttaccagagc cacaagaatt taattccttt tcgaccttca atccagttcc
      661 aaggactcca cgggcttgtc aaaattccca aaaatgatcc cctcaatgaa gttcataact
      721 ttaactttta tttgtcaaat gtgggcaaag acaaccctca gggcagcttt gactgcatcc
      781 agcaaacatt ctccagctct ggagcctccc agctcaattg cctgggattt atacaagata
      841 aaattacagt gtgtgcaaca aacgactcgt atcagatgac acgagaaaga atgacccagg
      901 cagaggagga atcccgcaac cgaagcacaa aagttatcaa acccggtgga ccatatgtag
      961 ggaaaagagt gcaaattcgg aaagcacctc aagctgtttc agatacagtt cctgagagga
     1021 aaaggtcaac ccccatgaac cctgcaaata caattcgaaa gacacatagc agcagcacca
     1081 tctctcagag gccatacagg gacagggtga ttcacttact ggccctgaag gcctacaaga
     1141 aaccggagct acttgctaga ctccagaaag atggtgtcaa tcaaaaagac aagaactccc
     1201 tgggagcaat tctgcaacag gtagccaatc tgaattctaa ggacctctca tataccttaa
     1261 aggattatgt ttttaaagag cttcaaagag actggcctgg atacagtgaa atagacagac
     1321 ggtcattgga gtcagtgctc tctagggggt acccatacga cgtcccagac tacgctgcta
     1381 gaaaactaaa tccgtctcag aatgctacag gcaccagccg ttcagaatct cctgtatgtt
     1441 ctagtagaga tgctgtatct tctcctcaga aacggctttt ggattcagag tttattgatc
     1501 ctttaatgaa taaaaaagcc cgaatatctc acctgacgaa cagagtacca ccaacactaa
     1561 atggtcattt gaatcccacc agtgaaaaat cggctgcagg cctcccactg ccccctgcgg
     1621 ctgctgccat ccccacccct ccaccgctgc cttcaaccta tctgcccatc tcacatcctc
     1681 ctcagattgt aaattctaac tccaactccc ctagcactcc agaaggccgg gggactcaag
     1741 acctacctgt tgacagtttt agtcaaaacg atagtatcta tgaggaccag caagacaaat
     1801 atacctctag gacttctctg gaaaccttac cccctggttc cgttctacta aagtgtccaa
     1861 agcctatgga agaaaaccat tcaatgtctc acaaaaagtc caaaaagaag tctaaaaaac
     1921 ataaggaaaa ggaccaaata aaaaagcacg acattgagac tattgaggaa aaggaggaag
     1981 atcttaagag agaagaggaa attgccaagc taaataactc cagtccaaat tccagtggag
     2041 gagttaaaga ggattgcact gcctccatgg aaccttcagc aattgaactc ccagattatt
     2101 tgataaaata tatcgctatc gtctcctatg agcaacgcca gaattataag gatgacttca
     2161 atgcagagta tgatgagtac agagctttgc atgccaggat ggagactgta gctagaagat
     2221 ttatcaaact agatgcacaa agaaagcgcc tttctccagg ctcaaaagag tatcagaatg
     2281 ttcatgaaga agtcttacaa gaatatcaga agataaagca gtctagtccc aattaccatg
     2341 aagaaaaata cagatgtgaa tatcttcata acaagctggc tcacatcaaa aggctaatag
     2401 gtgaatttga ccaacagcaa gcagagtcat ggtcctagaa ctctgcttgg accagaagat
     2461 gtgaataaac ttaagcctct ccagcgctgg atccggccat aagggcctga tccttcgagg
     2521 gggggcccgg taccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
     2581 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     2641 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     2701 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     2761 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2821 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2881 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2941 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3001 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3061 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3121 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3181 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3241 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3301 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3361 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3421 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3481 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3541 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3601 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3661 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3721 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3781 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3841 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3901 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3961 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4021 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4081 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4141 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4201 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4261 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4321 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4381 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4441 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4501 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4561 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4621 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4681 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     4741 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     4801 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     4861 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     4921 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     4981 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     5041 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     5101 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     5161 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
     5221 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     5281 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     5341 gacggccagt gaattg
//
