LOCUS       sequence-249698-       11963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-express EGFP-FLAG-cGAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11963)
  AUTHORS   Gentili M, Lahaye X, Nadalin F, Nader GPF, Puig Lombardi E, Herve S,
            De Silva NS, Rookhuizen DC, Zueva E, Goudot C, Maurin M, Bochnakian 
            A, Amigorena S, Piel M, Fachinetti D, Londono-Vallejo A, Manel N
  TITLE     The N-Terminal Domain of cGAS Determines Preferential Association 
            with Centromeric DNA and Innate Immune Activation in the Nucleus.
  JOURNAL   Cell Rep. 2019 Feb 26;26(9):2377-2393.e13. doi: 
            10.1016/j.celrep.2019.01.105.
  PUBMED    30811988
REFERENCE   2  (bases 1 to 11963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.01"; journalName: "Cell Rep"; date: 
            "2019-02-26- 26"; volume: "26"; issue: "9"; pages: "2377-2393.e13"
COMMENT     Sequence Label: pTRIP-SFFV-EGFP-FLAG-cGAS
FEATURES             Location/Qualifiers
     source          1..11963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          31..96
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             226..246
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    671..805
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(708..727)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     762..781
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             973..1606
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1653..1778
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2271..2504
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2689..2733
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3026..3143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3283..3690
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      3724..3733
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3730..3732,3733..3735,3736..4446)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3730..3732,3733..3735,3736..4446)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3775..3796)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4036..4055)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4383..4404
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4453..4476
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     LTR             6314..6494
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8022..8041)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8255..8274)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8374..8396
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8434..8452)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8520..8624
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8625..8693,8694..9485)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8843..8862)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9656..10244
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10145..10164
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10398..10415
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10485..10505)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10490..10819
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10670..10805
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
       61 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
      121 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
      181 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
      241 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
      301 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
      361 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
      421 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
      481 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
      541 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
      601 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
      661 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
      721 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
      781 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
      841 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
      901 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
      961 aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt
     1021 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     1081 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     1141 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     1201 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     1261 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     1321 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     1381 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     1441 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1501 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1561 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     1621 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     1681 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     1741 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     1801 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     1861 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1921 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1981 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     2041 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     2101 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     2161 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     2221 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     2281 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     2341 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     2401 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     2461 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     2521 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     2581 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     2641 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     2701 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     2761 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     2821 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     2881 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     2941 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccgaatt
     3001 cacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     3061 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     3121 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     3181 tgatacgcgt ctgcagcccc gataaaataa aagattttat ttagtctcca gaaaaagggg
     3241 ggaatgaaag accccacctg taggtttggc aagctagctg cagtaacgcc attttgcaag
     3301 gcatggaaaa ataccaaacc aagaatagag aagttcagat caagggcggg tacatgaaaa
     3361 tagctaacgt tgggccaaac aggatatctg cggtgagcag tttcggcccc ggcccggggc
     3421 caagaacaga tggtcaccgc agtttcggcc ccggcccgag gccaagaaca gatggtcccc
     3481 agatatggcc caaccctcag cagtttctta agacccatca gatgtttcca ggctccccca
     3541 aggacctgaa atgaccctgc gccttatttg aattaaccaa tcagcctgct tctcgcttct
     3601 gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc acaacccctc actcggcgcg
     3661 ccagtcctcc gacagactga gtcgcccggg ccgcggccgc gggctagcgg atccccaccg
     3721 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     3781 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     3841 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     3901 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     3961 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     4021 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     4081 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     4141 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     4201 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     4261 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     4321 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     4381 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     4441 tacaagacta gtgactacaa agacgatgac gacaagatgc agccttggca cggaaaggcc
     4501 atgcagagag cttccgaggc cggagccact gcccccaagg cttccgcacg gaatgccagg
     4561 ggcgccccga tggatcccaa cgagtctccg gctgcccccg aggccgccct gcctaaggcg
     4621 ggaaagttcg gccccgccag gaagtcggga tcccggcaga aaaagagcgc cccggacacc
     4681 caggagaggc cgcccgtccg cgcaactggg gcccgcgcca aaaaggcccc tcagcgcgcc
     4741 caggacacgc agccgtctga cgccaccagc gcccctgggg cagaggggct ggagcctcct
     4801 gcggctcggg agccggctct ttccagggct ggttcttgcc gccagagggg cgcgcgctgc
     4861 tccacgaagc caagaccccc gcccgggccc tgggacgtgc ccagccccgg cctgccggtc
     4921 tcggccccca ttctcgtacg gagggatgcg gcgcctgggg cctcgaagct ccgggcggtt
     4981 ttggagaagt tgaagctcag ccgcgatgat atctccacgg cggcggggat ggtgaaaggg
     5041 gttgtggacc acctgctgct cagactgaag tgcgactccg cgttcagagg cgtcgggctg
     5101 ctgaacaccg ggagctacta tgagcacgtg aagatttctg cacctaatga atttgatgtc
     5161 atgtttaaac tggaagtccc cagaattcaa ctagaagaat attccaacac tcgtgcatat
     5221 tactttgtga aatttaaaag aaatccgaaa gaaaatcatc tgagtcagtt tttagaaggt
     5281 gaaatattat cagcttctaa gatgctgtca aagtttagga aaatcattaa ggaagaaatt
     5341 aacgacatta aagatacaga tgtcatcatg aagaggaaaa gaggagggag ccctgctgta
     5401 acacttctta ttagtgaaaa aatatctgtg gatataaccc tggctttgga atcaaaaagt
     5461 agctggcctg ctagcaccca agaaggcctg cgcattcaaa actggctttc agcaaaagtt
     5521 aggaagcaac tacgactaaa gccattttac cttgtaccca agcatgcaaa ggaaggaaat
     5581 ggtttccaag aagaaacatg gcggctatcc ttctctcaca tcgaaaagga aattttgaac
     5641 aatcatggaa aatctaaaac gtgctgtgaa aacaaagaag agaaatgttg caggaaagat
     5701 tgtttaaaac taatgaaata ccttttagaa cagctgaaag aaaggtttaa agacaaaaaa
     5761 catctggata aattctcttc ttatcatgtg aaaactgcct tctttcacgt atgtacccag
     5821 aaccctcaag acagtcagtg ggaccgcaaa gacctgggcc tctgctttga taactgcgtg
     5881 acatactttc ttcagtgcct caggacagaa aaacttgaga attattttat tcctgaattc
     5941 aatctattct ctagcaactt aattgacaaa agaagtaaag aatttctgac aaagcaaatt
     6001 gaatatgaaa gaaacaatga gtttccagtt tttgatgaat tttgactcga gacctagaaa
     6061 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     6121 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     6181 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6241 ggctaattca ctcccaacga agacaaaatc gtcgagagat gctgcatata agcagctgct
     6301 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6361 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6421 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6481 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     6541 gaaatgaata tcagagagtg agaggccttg acattataat agatttagca ggaattgaac
     6601 taggagtgga gcacacaggc aaagctgcag aagtacttgg aagaagccac cagagatact
     6661 cacgattctg cacatacctg gctaatccca gatcctaagg attacattaa gtttactaac
     6721 atttatataa tgatttatag tttaaagtat aaacttatct aatttactat tctgacagat
     6781 attaattaat cctcaaatat cataagagat gattactatt atccccattt aacacaagag
     6841 gaaactgaga gggaaagatg ttgaagtaat tttcccacaa ttacagcatc cgttagttac
     6901 gactctatga tcttctgaca caaattccat ttactcctca ccctatgact cagtcgaata
     6961 tatcaaagtt atggacatta tgctaagtaa caaattaccc ttttatatag taaatactga
     7021 gtagattgag agaagaaatt gtttgcaaac ctgaatagct tcaagaagaa gagaagtgag
     7081 gataagaata acagttgtca tttaacaagt tttaacaagt aacttggtta gaaagggatt
     7141 caaatgcata aagcaaggga taaatttttc tggcaacaag actatacaat ataaccttaa
     7201 atatgacttc aaataattgt tggaacttga taaaactaat taaatattat tgaagattat
     7261 caatattata aatgtaattt acttttaaaa agggaacata gaaatgtgta tcattagagt
     7321 agaaaacaat ccttattatc acaatttgtc aaaacaagtt tgttattaac acaagtagaa
     7381 tactgcattc aattaagttg actgcagatt ttgtgttttg ttaaaattag aaagagataa
     7441 caacaatttg aattattgaa agtaacatgt aaatagttct acatacgttc ttttgacatc
     7501 ttgttcaatc attgatcgaa gttctttatc ttggaagaat ttgttccaaa gactctgaaa
     7561 taaggaaaac aatctattat atagtctcac acctttgttt tacttttagt gatttcaatt
     7621 taataatgta aatggttaaa atttattctt ctctgagatc atttcacatt gcagatagaa
     7681 aacctgagac tggggtaatt tttattaaaa tctaatttaa tctcagaaac acatctttat
     7741 tctaacatca atttttccag tttgatatta tcatataaag tcagccttcc tcatctgcag
     7801 gttccacaac aaaaatccaa ccaactgtgg atcaaaaata ttgggaaaaa attaaaaata
     7861 gcaatacaac aataaaaaaa tacaaatcag aaaaacagca cagtataaca actttattta
     7921 gcatttacaa tctattaggt attataagta atctagccag atcctctacg ccggacgcat
     7981 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
     8041 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
     8101 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
     8161 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
     8221 gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     8281 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     8341 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     8401 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     8461 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     8521 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     8581 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     8641 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     8701 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     8761 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     8821 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     8881 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     8941 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     9001 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     9061 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     9121 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     9181 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     9241 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     9301 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     9361 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     9421 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     9481 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     9541 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     9601 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     9661 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     9721 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     9781 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     9841 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     9901 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     9961 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    10021 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    10081 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    10141 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    10201 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    10261 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    10321 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    10381 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    10441 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt
    10501 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
    10561 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    10621 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
    10681 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
    10741 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
    10801 aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa
    10861 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
    10921 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
    10981 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
    11041 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
    11101 aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg
    11161 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
    11221 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
    11281 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
    11341 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
    11401 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
    11461 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
    11521 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
    11581 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
    11641 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
    11701 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg
    11761 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
    11821 tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc
    11881 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
    11941 taaagctcta aggtaaatat aaa
//
