LOCUS       sequence-249754-       12379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses gRNA in Aedes aegypti.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12379)
  AUTHORS   Li M, Yang T, Kandul NP, Bui M, Gamez S, Raban R, Bennett J, Sanchez
            C HM, Lanzaro GC, Schmidt H, Lee Y, Marshall JM, Akbari OS
  TITLE     Development of a confinable gene drive system in the human disease 
            vector Aedes aegypti.
  JOURNAL   Elife. 2020 Jan 21;9. pii: 51701. doi: 10.7554/eLife.51701.
  PUBMED    31960794
REFERENCE   2  (bases 1 to 12379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-01-21"; volume: "9. pii: 51701. doi"; pages: " 
            10.7554/eLife.51701"
COMMENT     Sequence Label: U6c
FEATURES             Location/Qualifiers
     source          1..12379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..681)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     polyA_signal    795..916
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   1584..1618
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(1799..1815)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1799..1815)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1812..1834)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1823..1839
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1847..1853,1854..1871,1872..1877))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1892..1913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2030..2047)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2201..2789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2281..2300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2960..3751,3752..3820))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3583..3602
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3821..3925)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3993..4011
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4049..4071)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4171..4190
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4384..4406
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4398..4415
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4399..4415
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   5119..5181
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      8959..8968
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    10517..10638
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 atggtgcgct cctccaagaa cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag
       61 ggcaccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      121 ggccacaaca ccgtgaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
      181 atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc
      241 cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      301 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg ctgcttcatc
      361 tacaaggtga agttcatcgg cgtgaacttc ccctccgacg gccccgtaat gcagaagaag
      421 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
      481 gagatccaca aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagtcc
      541 atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga ctccaagctg
      601 gacatcacct cccacaacga ggactacacc atcgtggagc agtacgagcg caccgagggc
      661 cgccaccacc tgttcctgta ggactctaga tcataatcag ccataccaca tttgtagagg
      721 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
      781 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
      841 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      901 tcatcaatgt atcttaaagc ttctaggccg gccgatctcg gatctgacaa tgttcagtgc
      961 agagactcgg ctacgcctcg tggactttga agttgaccaa caatgtttat tcttacctct
     1021 aatagtcctc tgtggcaagg tcaagattct gttagaagcc aatgaagaac ctggttgttc
     1081 aataacattt tgttcgtcta atatttcact accgcttgac gttggctgca cttcatgtac
     1141 ctcatctata aacgcttctt ctgtatcgct ctggacgtca tcttcactta cgtgatctga
     1201 tatttcactg tcagaatcct caccaacaag ctcgtcatcg ctttgcagaa gagcagagag
     1261 gatatgctca tcgtctaaag aactacccat tttattatat attagtcacg atatctataa
     1321 caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt
     1381 atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac
     1441 gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga
     1501 gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa
     1561 gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt
     1621 aaaaaagatt tgcgctttac tcgacctaaa ctttaaacac gtcatagaat cttcgtttga
     1681 caaaaaccac attgtggcca agctgtgtga cgcgacgcgc gctaaagaat ggcaaaccaa
     1741 gtcgcgcgag cgtcgactct agaggatccc cgggtaccga gctcgaattc gtaatcatgt
     1801 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     1861 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     1921 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     1981 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     2041 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     2101 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2161 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2221 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2281 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2341 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2401 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2461 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2521 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2581 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2641 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2701 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2761 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2821 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2881 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2941 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     3001 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     3061 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     3121 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3181 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3241 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     3301 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3361 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3421 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3481 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3541 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3601 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3661 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3721 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3781 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3841 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3901 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     3961 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     4021 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     4081 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     4141 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     4201 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     4261 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     4321 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     4381 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctttgttta aaaatataac
     4441 aaaattgtga tcccacaaaa tgaagtgggg caaaatcaaa taattaatag tgtccgtaaa
     4501 cttgttggtc ttcaactttt tgaggaacac gttggacggc aaatccgtga ctataacaca
     4561 agttgattta ataattttag ccaacacgtc gggctgcgtg ttttttgccg acgcgtctgt
     4621 gtacacgttg attaactggt cgattaaact gttgaaataa tttaattttt ggttcttctt
     4681 taaatctgtg atgaaatttt ttaaaataac tttaaattct tcattggtaa aaaatgccac
     4741 gttttgcaac ttgtgagggt ctaatatgag gtcaaactca gtaggagttt tatccaaaaa
     4801 agaaaacatg attacgtctg tacacgaacg cgtattaacg cagagtgcaa agtataagag
     4861 ggttaaaaaa tatattttac gcaccatata cgcatcgggt tgatatcgtt aatatggatc
     4921 aatttgaaca gttgattaac gtgtctctgc tcaagtcttt gatcaaaacg caaatcgacg
     4981 aaaatgtgtc ggacaatatc aagtcgatga gcgaaaaact aaaaaggcta gaatacgaca
     5041 atctcacaga cagcgttgag atatacggta ttcacgacag caggctgaat aataaaaaaa
     5101 ttagaaacta ttatttaacc ctagaaagat aatcatattg tgacgtacgt taaagataat
     5161 catgcgtaaa attgacgcat gtgttttatc ggtctgtata tcgaggttta tttattaatt
     5221 tgaatagata ttaagtttta ttatatttac acttacatac taataataaa ttcaacaaac
     5281 aatttattta tgtttattta tttattaaaa aaaaacaaaa actcaaaatt tcttctataa
     5341 agtaacaaaa cttttaaaca ttctctcttt tacaaaaata aacttatttt gtactttaaa
     5401 aacagtcatg ttgtattata aaataagtaa ttagcttaac ttatacataa tagaaacaaa
     5461 ttatacttat tagtcagtca gaaacaactt tggcacatat caatattatg ctctcgacaa
     5521 ataacttttt tgcatttttt gcacgatgca tttgcctttc gccttatttt agaggggcag
     5581 taagtacagt aagtacgttt tttcattact ggctcttcag tactgtcatc tgatgtacca
     5641 ggcacttcat ttggcaaaat attagagata ttatcgcgca aatatctctt caaagtagga
     5701 gcttctaaac gcttacgcat aaacgatgac gtcaggctca tgtaaaggtt tctcataaat
     5761 tttttgcgac tttgaacctt ttctcccttg ctactgacat tatggctgta tataataaaa
     5821 gaatttatgc aggcaatgtt tatcattccg tacaataatg ccataggcca cctattcgtc
     5881 ttcctactgc aggtcatcac agaacacatt tggtctagcg tgtccactcc gcctttagtt
     5941 tgattataat acataaccat ttgcggttta ccggtacttt cgttgataga agcatcctca
     6001 tcacaagatg ataataagta taccatctta gctggcttcg gtttatatga gacgagagta
     6061 aggggtccgt caaaacaaaa catcgatgtt cccactggcc tggagcgact gtttttcagt
     6121 acttccggta tctcgcgttt gtttgatcgc acggttccca caatggttaa ttcgagctcg
     6181 cccggggtcc taggcctcac gccactcgta gaatgaaccc gattgaatca tttttaaacc
     6241 gaaaaaaaaa tcctgccggt gttctctgag ttttctgagg attctgatcg ggatcccaga
     6301 ggaacctggg aattgctatt ggattcctaa cagactcttg agaattacca tcagagtttc
     6361 gtacattttt aacaagattt tttgtggaat catcaccaaa ccatgaaaat ttctagcagg
     6421 cttttggggt tcctcagtgg gatcatgaaa ctccgttaca tcgtttttgc tatgagatta
     6481 cagaaaaaat aacttaagta aacagttttt ctgtaaaaga tatttcactc acttagcttt
     6541 tgaacgcagt ggaattttag accattgttt gcttgacttt ttttttaatc ataatttaaa
     6601 tgttagagtg ttagaaaccg tgttcttata aacaatattc tccttttctt cttccctgtc
     6661 ttgcgaaaca aacgatagag agaaacttat cagtgtacct ggcgtctaag ctgcgaatca
     6721 aggaactacg attttccagc cacttcctat gtaaatcaat cgaagtatcg tttagtacat
     6781 tacatttaat acatttcttc ttcatctatg catctttgac ttttccaaaa tgaatatcct
     6841 atttcattat acattttttc ttttacttcc agcgctacag caccagcagt tatcaggatg
     6901 tagatgaagg tatcaacagt tcgttcggca gcaatgacaa atccacgctg attcaggttt
     6961 ggaaacccaa aagttatggc gccgtcaagg gacaaattcc acagcacgaa cgactcacat
     7021 acacatggaa ggagatcgat gtgtttggag agacgccagg tgataccaaa aaggaacccc
     7081 tgtgcagtag attgtgctgt tgtttctccc gccagaagaa agacttcaat ccccgcaaac
     7141 acctactgaa gaatgtgacc gggatggcta agagtggaga gttactggct gtgatgggta
     7201 gttctggcgc cggtaaaaca accttgctga acgctttgct agcatatgtc ttgccgaagc
     7261 acattaaccc aagccattta tatatcaagt acaagttgat ttgattctag tgatggactg
     7321 acacgaatct atggcactgt tgcagagctt acaaacaggt acccggagtc gcttcaatta
     7381 ttcaatcatc cattcaaaaa cttccatctc ttccaattag ccaatattca aattggaatc
     7441 cagccagccg acggaggcag atggtctacc tggcaactat agagtttcca tgtttccaga
     7501 ctttccctcc cggtaaacgg agacaaaacg acagacgtaa gtaggtacat atgcataccg
     7561 cacggacaaa tcaaatttgt ctggcagctc caattagagt cgttaaaaat ttaacgatgc
     7621 gctaaataac ttcaagctat ttgtctcgct ggattggctt cgagtggtaa gatcctatca
     7681 aatgccgaaa acaaaaaact ttcttcttaa ttgttcgttc ttcaacacct ctccatggtg
     7741 ataacggata cggtttcatt gtcagcatcc atcctccgaa aaatacatta cgccttgaaa
     7801 tatgcaatcg caaacacgga tctgtttgga acatttattt tactatgaag agatgcgata
     7861 ggtaatattt atttgagcgt ttaagatact cattgttctc tcaaagaatg tcattgaaag
     7921 ccaacgaggt caaatcaaat attataataa aaaggtcaaa gaggactaac ttaaagctct
     7981 ctttatggat aggaaaaaat attttcgccc atcgctagaa cttttaccgt ttccattgag
     8041 tatataacta agatgaatga ggctaattga tggtgcgatc ttgacccccg ggtcttagag
     8101 ctatgctgga aacagcatag caagttaaga taaggctagt ccgttatcaa cttgaaaaag
     8161 tggcaccgag tcggtgcttt ttttaagatg cttcggcaat taataataag gaatggtaaa
     8221 gcattcttca aatgttacta tgttctcaaa ttttcaagaa ttaggtattc aagaaattct
     8281 ttcaggcata ttccataatc tgcacaagca gctactttag aaattattgc cgggcttaaa
     8341 ttatatgtac aacatttcct gagtattttc tgacaaaaat tctcacaagg gaaggtcacg
     8401 atggagttac acggggtttt taaccggact atttttgtta gattctacat gtcgaaatat
     8461 gaaaaacccc aaagcctttc gggtgatgga ccagtggtgt agccaggagg ggctctgaaa
     8521 ggggtaattt tgtacgtata ttatattatt aagctaattg gaaatttgac aaaaatattt
     8581 ttttagtatt tattgtgatg gtagagaaca aaattgacat taatgtacgt ttgaaatgta
     8641 tttgccatgc ctttggcgta ctaacatgga gtatggacac caaaaccgat ttcgttaatt
     8701 aattcgagct cgcccgggga tctaattcaa ttagagacta attcaattag agctaattca
     8761 attaggatcc aagcttatcg atttcgaacc ctcgaccgcc ggagtataaa tagaggcgct
     8821 tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc taagcgaaag
     8881 ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat cggggtaccg ctagagtcga
     8941 cggtacgatc caccggtcgc caccatggtg agcaagggcg aggaggtcat caaagagttc
     9001 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     9061 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     9121 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
     9181 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
     9241 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
     9301 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
     9361 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     9421 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
     9481 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
     9541 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     9601 gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg caccggcagc
     9661 accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc cgttatcaag
     9721 gaatttatgc gcttcaaagt taggatggag ggatccatga acggacatga gttcgagatc
     9781 gagggagagg gcgagggacg cccgtatgaa ggcacacaaa cagccaaact caaggtcacc
     9841 aagggcggac cactgccctt cgcctgggat atcctgagtc cccagtttat gtacggcagc
     9901 aaggcctacg ttaagcaccc cgctgacata ccggactaca aaaagctgtc ctttccggaa
     9961 ggcttcaagt gggagcgcgt gatgaatttc gaagacggag gactggtcac tgtgacccaa
    10021 gatagcagtt tgcaggacgg tacactgatc tataaggtta aaatgcgcgg cactaacttt
    10081 ccgccagatg gcccagtgat gcagaagaag accatgggtt gggaggcatc caccgaacgt
    10141 ctgtaccctc gagacggagt gctcaagggc gagatccatc aggccctcaa actgaaagat
    10201 ggtggtcact acctggtcga atttaagacc atttacatgg ccaagaagcc ggttcagctg
    10261 cccggatatt attatgtgga tacgaaactg gatataactt cgcataacga agactacacc
    10321 attgtcgagc agtatgagcg cagcgaaggc cgacatcacc tgttcctcta cggcatggac
    10381 gagctgtaca agtaggcggc cgcgactcta gatcataatc agccatacca catttgtaga
    10441 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
    10501 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    10561 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    10621 actcatcaat gtatcttaaa gcttgtttaa actcaagatc gcaccgactt cagtacgtgc
    10681 cctaaacgga atcccagtca acgcggaaca gttgcgagca cgttgcgcct atgtgcaaca
    10741 ggacgatctt ttcattcccg cgcttacaac tcgagaacac ctcgtattcc acgcaatgct
    10801 gcgtatgggt aaagatgtac ccaagagtgt caagatgaat cgagtcaacg aggtgcttca
    10861 ggagctttcc ttagcgaaat gcgcagacac gatcattgga gcgccaggtc gaatgaaagg
    10921 cctgtccgga ggtgaacgga aacggttggc ctttgcatcg gaaaccctga ccgatcctca
    10981 cctgttgctt tgtgatgaac ccacttccgg gttggattcg ttcatggctc acagtgttct
    11041 acaggtgctg aagggaatgg ccctcaaagg aaagacgatt atactgacca tacatcaacc
    11101 ttcgtcagaa ctgtattgct tgttcgacaa aattctccta gtggcagaag gacgcgtggc
    11161 tttccttgga tctccctatc aggcttcgga attcttttct cagtgagtag acttgtgaaa
    11221 tcctgacgat tttgctttac tagcagggct tgtggtgtct tgcctcttaa aacaccgaac
    11281 atgaaaccca aaagccaaac gcaataggaa tcagacgatg agagttcttc atagatctga
    11341 ccagacatgt ctctaagaat atttctaaga cttttttttt ggaaattcat cgcgatcttg
    11401 acattacgcg taatccgcgt aatcatcgta tttttccaaa gttacattca gatattacgg
    11461 cagaaattct ttaggagatt tcatcaggag ataacatatt ggtgaattac ggattcgttg
    11521 tatttatctt caagaatact acctgatttt agtttagcaa ttacttcaga gatttcattt
    11581 agaattcaag attcatgcag aaatattctt tcattgatag aacttccttc tatgcagaac
    11641 atctaccaga attcaaccag aaatttctac ttatatacct ctaacacggc caattgcggc
    11701 cagacagtaa aataatagtg gccttagtaa ctaaaatgat caaaattgtg acccggtaaa
    11761 gtgatttaat aaaacagttt ttagtccaaa caatattagg ggtcatccaa aaacgacttc
    11821 aatcattagg ggcgcgccca tactcggtgg cctccccacc accaactttt ttgcactgca
    11881 aaaaaacacg cttttgcacg cgggcccata catagtacaa actctacgtt tcgtagacta
    11941 ttttacataa atagtctaca ccgttgtata cgctccaaat acactaccac acattgaacc
    12001 tttttgcagt gcaaaaaagt acgtgtcggc agtcacgtag gccggcctta tcgggtcgcg
    12061 tcctgtcacg tacgaatcac attatcggac cggacgagtg ttgtcttatc gtgacaggac
    12121 gccagcttcc tgtgttgcta accgcagccg gacgcaactc cttatcggaa caggacgcgc
    12181 ctccatatca gccgcgcgtt atctcatgcg cgtgaccgga cacgaggcgc ccgtcccgct
    12241 tatcgcgcct ataaatacag cccgcaacga tctggtaaac acagttgaac agcatctgtt
    12301 acagcgacac aacatgagcc ggtccaacaa cgccaacgcg cccacgccat ccaaccgccg
    12361 ccgcaacctg tctctggtg
//
