LOCUS       sequence-249755-        1706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes spacer sequence as a Type 4 part to be used in the MTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK4_004
FEATURES             Location/Qualifiers
     source          1..1706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     211..230
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(482..516)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(587..1246)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1161..1180
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1428..1706,1..310)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
       61 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
      121 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      181 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      241 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      301 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      361 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta gtcggtctca
      421 atcctaaact agagatctat gtgaggatcc taactcgaga tcgagctgtg agaccagacc
      481 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
      541 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
      601 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
      661 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
      721 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
      781 aactggtgaa actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt
      841 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
      901 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
      961 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     1021 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     1081 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     1141 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     1201 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     1261 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     1321 gaaagttgga acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt
     1381 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1441 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1501 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1561 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1621 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1681 aagtcgtgtc ttaccgggtt ggactc
//
