LOCUS       sequence-24976-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Zhang Y, Nash L, Fisher AL.
  TITLE     A simplified, robust, and streamlined procedure for the production 
            of C. elegans transgenes via recombineering
  JOURNAL   BMC Dev Biol. 2008 Dec 30;8(1):119.
  PUBMED    19116030
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2008-12-30"; volume: "8(1)"; pages: "119"
COMMENT     Sequence Label: pMOD4 galK-G
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(202..306)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     374..392
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(430..452)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     552..571
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    710..728
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
ORIGIN
        1 angnnncnng nnaacgttct tcggggcgaa actctcaagg atcttaccgc tgttgagatc
       61 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      121 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      181 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      241 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      301 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
      361 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
      421 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
      481 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
      541 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
      601 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
      661 caactgttgg gaanggcgat cggtgcgggc ctcttcgcta ttacgacagc tgtctcttat
      721 acacatctca accatcatcg atgaattttt ctcgggtgtt ctcgcata
//
