LOCUS       sequence-249771-        2440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes mScarlet as a Type 4a part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK4a_012
FEATURES             Location/Qualifiers
     source          1..2440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(805..839)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(910..1569)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1484..1503
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1751..2339
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2240..2259
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa tccgtatcaa aaggcgaggc ggtcattaaa gagttcatga ggtttaaagt
       61 ccatatggaa ggaagcatga atggtcacga gtttgagata gagggagagg gtgagggaag
      121 gccgtacgaa gggacgcaaa ctgcgaagtt gaaggtaacg aaaggcggcc ctctgccatt
      181 ctcttgggac atcctttcac cgcaatttat gtacggctct agggccttca caaaacaccc
      241 cgccgatatt ccggattact ataaacaaag ttttcctgag ggcttcaagt gggaaagagt
      301 aatgaacttc gaggatgggg gagctgtgac ggttactcaa gacactagcc tggaggacgg
      361 tactcttata tacaaggtaa aactaagagg gacgaatttt ccgccagacg gccccgtgat
      421 gcaaaaaaaa actatggggt gggaagcatc caccgagagg ctgtaccccg aagacggggt
      481 cctgaagggt gatattaaaa tggcattaag attaaaagat ggcgggagat acttagctga
      541 cttcaagact acatataaag ccaaaaaacc tgtacagatg cctggagctt acaatgtgga
      601 tcgtaagctg gatattacat cccacaacga agattatacc gtggtggagc agtacgagcg
      661 ttcagagggg cgtcactcca ccgggggtat ggatgaatta tacaagagcg gtcttagaag
      721 cagggcgcaa gcgtcaaatt cagcggtaga tggaacagca ggtccaggta gtactggcag
      781 cagataatgg ctgagaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac
      841 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
      901 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
      961 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     1021 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     1081 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa
     1141 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     1201 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     1261 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     1321 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
     1381 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     1441 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     1501 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     1561 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     1621 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga
     1681 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1741 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1801 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1861 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1921 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1981 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2041 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2101 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2161 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2221 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2281 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2341 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2401 tctttcctgc gttatcccct gattctgtgg ataaccgtag
//
