LOCUS       sequence-249972-        2269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the 5' 500 bp cRosa26 locus homology arm as a type 7 part to
            be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK7_009
FEATURES             Location/Qualifiers
     source          1..2269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      77..665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     566..585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1400..1434)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1505..2164)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2079..2098
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg tctcagagta
      781 gatcgcgggg gagggaggga ttcttctagg cccagggcgg tccttaggag acaggaggca
      841 gccgagaact cccataaagg tattgcggca ctcccctccc cctaccgaga agggtgcggc
      901 cttctctccg cctcctccac tgcagatccc tcaggattgc agctcgcgcc gattttcaga
      961 gatcacgcac ctccctccca caaaccagca ggaccgaccc ccgctcctcc ccccacctcc
     1021 gcgagtccgc gagcggatcc agctcatctc gtccttcagg cggcggacgc ccgggacgga
     1081 gccgcggtcg ccgccaccca gaagcctcga cccagcagtc cgcctccgcc tccgcctccg
     1141 cctctagtgc gtgcgcaggg gcgcggggcc agacactgcg gcgcggggcc tcgggaagga
     1201 ccgtaccaac gaccgcctcc ctgcccctcc gccccctctg ctgacccgct cagtggtttc
     1261 gctcaattgg gacccgagcc aatggcaagg ggccgttccc cggcttcagc agccaatcag
     1321 gtgccgaagc cacacgggcg gcgggtaacc ctactccccg cgagggtttt cccagtcacg
     1381 acccgatgag accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     1441 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
     1501 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     1561 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     1621 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     1681 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca
     1741 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     1801 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     1861 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     1921 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
     1981 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     2041 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     2101 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     2161 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     2221 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaa
//
