LOCUS       sequence-249981-        1107 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human Preprotachykinin A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1107)
  TITLE     Michael Kressel plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Preprotachykinin A
FEATURES             Location/Qualifiers
     source          1..1107
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      403..412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             409..1107
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 atgaaaatcc tcgtggcctt ggcagtcttt tttcttgtct ccactcagct gtttgcagaa
       61 gaaataggag ccaatgatga tctgaattac tggtccgact ggtacgacag cgaccagatc
      121 aaggaggaac tgccggagcc ctttgagcat cttctgcaga gaatcgcccg gagacccaag
      181 cctcagcagt tctttggatt aatgggcaaa cgggatgctg attcctcaat tgaaaaacaa
      241 gtggccctgt taaaggctct ttatggacat ggccagatct ctcacaaaag acataaaaca
      301 gattcctttg ttggactaat gggcaaaaga gctttaaatt ctgtggctta tgaaaggagt
      361 gcaatgcaga attatgaaag aagacgtggg gatccaccgg tcgccaccat ggtgagcgag
      421 ctgattaagg agaacatgcc catgaagctg tacatggagg gcaccgtgaa caaccaccac
      481 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
      541 aaggtcgtcg agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg
      601 tacggcagca gaaccttcat caagcaccct ccgggcatcc ccgacttctt taagcagtcc
      661 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
      721 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa ggttagaggg
      781 gtgaacttcc cagccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggcctcc
      841 accgagacga tgtaccccgc tgacggcggc ctggaaggcg catgtgacat ggccctgaag
      901 ctcgtgggcg ggggccacct gatctgcaac cttgagacca catacagatc caagaaaccc
      961 gctacgaacc tcaagatgcc cggcgtctac aacgtggacc acagactgga aagaatcaag
     1021 gaggccgacg atgagaccta cgtcgagcag cacgaggtgg ctgtggccag atactctact
     1081 ggtggcgctg gtgatggagg taaatga
//
