LOCUS       sequence-250005-        9448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Helper plasmid for production of phagemid ssDNA, based on M13KO7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Praetorius F, Kick B, Behler KL, Honemann MN, Weuster-Botz D, Dietz 
            H
  TITLE     Biotechnological mass production of DNA origami.
  JOURNAL   Nature. 2017 Dec 6;552(7683):84-87. doi: 10.1038/nature24650.
  PUBMED    29219963
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2017-12-6- 6"; volume: "552"; issue: "7683"; pages:
            "84-87"
COMMENT     Sequence Label: HP17_KO7
FEATURES             Location/Qualifiers
     source          1..9448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     686..705
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     770..789
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(1136..2086)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFGIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2134..2355
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2847..2941)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(3097..3126)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             3337..4569
                     /codon_start=1
                     /gene="II"
                     /product="pII"
                     /label=M13 gene II
                     /note="replication"
                     /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGMNLSAR
                     TVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGHNV
                     FGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVIDHL
                     RNVSNGQTKSTRSQNWESTVTWNETSRHRTLVAYLKHVELQHQIQQLSSKPSAKMTSYQ
                     KEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQGKDLIF
                     DLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRL
                     VNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRP
                     DWYNEPVLKIA"
     CDS             4581..4844
                     /codon_start=1
                     /gene="V"
                     /product="pV"
                     /label=M13 gene V
                     /note="replication"
                     /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVLV
                     KITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK"
     CDS             4846..4947
                     /codon_start=1
                     /gene="VII"
                     /product="pVII"
                     /label=M13 gene VII
                     /note="minor coat protein"
                     /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR"
     CDS             4944..5042
                     /codon_start=1
                     /gene="IX"
                     /product="pIX"
                     /label=M13 gene IX
                     /note="minor coat protein"
                     /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
     CDS             5039..5260
                     /codon_start=1
                     /gene="VIII"
                     /product="pVIII"
                     /label=M13 gene VIII
                     /note="major coat protein"
                     /translation="MKKSLALKASVAVATLVPMLSFAAEGDDPAKAAFNSLQASATEYI
                     GYAWAMVVVIVGATIGIKLFKKFTSKAS"
     terminator      5264..5312
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             5317..6591
                     /codon_start=1
                     /gene="III"
                     /product="pIII"
                     /label=M13 gene III
                     /note="minor coat protein"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     CDS             6594..6932
                     /codon_start=1
                     /gene="VI"
                     /product="pVI"
                     /label=M13 gene VI
                     /note="minor coat protein"
                     /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFLA
                     LIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQKI
                     VSYLDWDK"
     CDS             6934..7980
                     /codon_start=1
                     /gene="I"
                     /product="pI"
                     /label=M13 gene I
                     /note="phage assembly"
                     /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLPQ
                     VGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDKER
                     QPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTLYSL
                     ITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGVYSYL
                     TPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPEVKKVV
                     SQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTVSIKKGN
                     SNEIVKCN"
     CDS             7958..9238
                     /codon_start=1
                     /gene="IV"
                     /product="pIV"
                     /label=M13 gene IV
                     /note="phage assembly"
                     /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGES
                     VIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSNPSIIQKYNPNNQDYIDE
                     LPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKSSNV
                     LSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFAAGSQ
                     RGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRILTLSGQ
                     KGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIVLDITSK
                     ADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFLSKIPLIG
                     LLFSSRSDSNEESTLYVLVKATIVRAL"
     terminator      9281..9312
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(join(9411..9448,1..778))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
       61 aaaactcacc gaggcagttc caaagaatgg caaggtcctg gtaacggtct gcgattccga
      121 cccgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
      181 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caagagcttg tgcatttctt
      241 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
      301 aaccgttatt catgcgtgat tgcgcctgag caagacgaaa tacacgatcg ctgttaaaag
      361 gacaattaca aacaggaatc gaatgtaacc ggcgcaggaa cacggccagc gcatcaacaa
      421 tattttcacc tgaatcagga tattcttcta atacctggaa ggctgttttc ccaggaatcg
      481 cggtggtgag taaccacgca tcatcaggag tacggataaa atgcttgatg gtcgggagag
      541 gcataaactc cgtcagccag ttgagacgga ccatctcatc tgtaacatca ttggcaacgc
      601 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aagcgataga
      661 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcgt
      721 ccatgttgga gtttaagcgc ggacgggagc aagacgtttc ccgttgaata tggctcataa
      781 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt
      841 tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt tgaataaatc
      901 gaacttttgc tgagttgaag gatcagctct agtagttaca ttgtcgatct gttcatggtg
      961 aacagctttg aatgcaccaa aaactcgtaa aagctctgat gtatctatct tttttacacc
     1021 gttttcatct gtgcatatgg acagttttcc ctttgatatg taacggtgaa cagttgttct
     1081 acttttgttt gttagtcttg atgcttcact gatagataca agagccataa gaacctcaga
     1141 tccttccgta tttagccagt atgttctcta gtgtggttcg ttgtttttgc gtgagccatg
     1201 agaacgaacc attgagatca tacttacttt gcatgtcact caaaaatttt gcctcaaaac
     1261 tggtgagctg aatttttgca gttaaagcat cgtgtagtgt ttttcttagt ccgttatgta
     1321 ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc attcattttt atctggttgt
     1381 tctcaagttc ggttacgaga tccatttgtc tatctagttc aacttggaaa atcaacgtat
     1441 cagtcgggcg gcctcgctta tcaaccacca atttcatatt gctgtaagtg tttaaatctt
     1501 tacttattgg tttcaaaacc cattggttaa gccttttaaa ctcatggtag ttattttcaa
     1561 gcattaacat gaacttaaat tcatcaaggc taatctctat atttgccttg tgagttttct
     1621 tttgtgttag ttcttttaat aaccactcat aaatcctcat agagtatttg ttttcaaaag
     1681 acttaacatg ttccagatta tattttatga atttttttaa ctggaaaaga taaggcaata
     1741 tctcttcact aaaaactaat tctaattttt cgcttgagaa cttggcatag tttgtccact
     1801 ggaaaatccc aaagccttta accaaaggat tcctgatttc cacagttctc gtcatcagct
     1861 ctctggttgc tttagctaat acaccataag cattttccct actgatgttc atcatctgag
     1921 cgtattggtt ataagtgaac gataccgtcc gttctttcct tgtagggttt tcaatcgtgg
     1981 ggttgagtag tgccacacag cataaaatta gcttggtttc atgctccgtt aagtcatagc
     2041 gactaatcgc tagttcattt gctttgaaaa caactaattc agacatacat ctcaattggt
     2101 ctaggtgatt ttaatcacta taccaattga gatgggctag tcaatgataa ttacatgtcc
     2161 ttttcctttg agttgtgggt atctgtaaat tctgctagac ctttgctgga aaacttgtaa
     2221 attctgctag accctctgta aattccgcta gacctttgtg tgtttttttg tttatattca
     2281 agtggttata atttatagaa taaagaaaga ataaaaaaag ataaaaagaa tagatcccag
     2341 ccctgtgtat aactcactac tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac
     2401 gctgtttgct cctctacaaa acagacctta aaaccctaaa ggcttaagta gcaccctcgc
     2461 aagctcgggc aaatcgctga atattccttt tgtctccgac catcaggcac ctgagtcgct
     2521 gtctttttcg tgacattcag ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat
     2581 ggcactacag gcgcctttta tggattcatg caaggaaact acccataata caagaaaagc
     2641 ccgtcacggg cttctcaggg cgttttatgg cgggtctgct atgtggtgct atctgacttt
     2701 ttgctgttca gcagttcctg ccctctgatt ttccagtctg accacttcgg attatcccgt
     2761 gacaggtcat tcagactggc taatgcaccc agtaaggcag cggtatcatc aacaggctta
     2821 cccgtcttac tgtccctagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     2881 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     2941 ccaagcgagc tcgtaaactt ggtctgacag ctctagctcc ggcaaaaaaa cgggcaaggt
     3001 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgttt gccacctgac
     3061 gtctaagaaa aggaatattc agcaatttgc ccgtgccgaa gaaaggccca cccgtgaagg
     3121 tgagccagtg agttgattgc tacgtaatta gttagttagc ccttagtgac tccagacgcg
     3181 aattattttt gatggcgttc ctattggtta aaaaatgagc tgatttaaca aaaatttaat
     3241 gcgaatttta acaaaatatt aacgtttaca atttaaatat ttgcttatac aatcttcctg
     3301 tttttggggc ttttctgatt atcaaccggg gtacatatga ttgacatgct agttttacga
     3361 ttaccgttca tcgattctct tgtttgctcc agactctcag gcaatgacct gatagccttt
     3421 gtagacctct caaaaatagc taccctctcc ggcatgaatt tatcagctag aacggttgaa
     3481 tatcatattg atggtgattt gactgtctcc ggcctttctc acccttttga atctttacct
     3541 acacattact caggcattgc atttaaaata tatgagggtt ctaaaaattt ttatccttgc
     3601 gttgaaataa aggcttctcc cgcaaaagta ttacagggtc ataatgtttt tggtacaacc
     3661 gatttagctt tatgctctga ggctttattg cttaattttg ctaattcttt gccttgcctg
     3721 tatgatttat tggatgttaa cgctactact attagtagaa ttgatgccac cttttcagct
     3781 cgcgccccaa atgaaaatat agctaaacag gttattgacc atttgcgaaa tgtatctaat
     3841 ggtcaaacta aatctactcg ttcgcagaat tgggaatcaa ctgttacatg gaatgaaact
     3901 tccagacacc gtactttagt tgcatattta aaacatgttg agctacagca ccagattcag
     3961 caattaagct ctaagccatc cgcaaaaatg acctcttatc aaaaggagca attaaaggta
     4021 ctctctaatc ctgacctgtt ggagtttgct tccggtctgg ttcgctttga agctcgaatt
     4081 aaaacgcgat atttgaagtc tttcgggctt cctcttaatc tttttgatgc aatccgcttt
     4141 gcttctgact ataatagtca gggtaaagac ctgatttttg atttatggtc attctcgttt
     4201 tctgaactgt ttaaagcatt tgagggggat tcaatgaata tttatgacga ttccgcagta
     4261 ttggacgcta tccagtctaa acattttact attaccccct ctggcaaaac ttcttttgca
     4321 aaagcctctc gctattttgg tttttatcgt cgtctggtaa acgagggtta tgatagtgtt
     4381 gctcttacta tgcctcgtaa ttccttttgg cgttatgtat ctgcattagt tgaatgtggt
     4441 attcctaaat ctcaactgat gaatctttct acctgtaata atgttgttcc gttagttcgt
     4501 tttattaacg tagatttttc ttcccaacgt cctgactggt ataatgagcc agttcttaaa
     4561 atcgcataag gtaattcaca atgattaaag ttgaaattaa accatctcaa gcccaattta
     4621 ctactcgttc tggtgtttct cgtcagggca agccttattc actgaatgag cagctttgtt
     4681 acgttgattt gggtaatgaa tatccggttc ttgtcaagat tactcttgat gaaggtcagc
     4741 cagcctatgc gcctggtctg tacaccgttc atctgtcctc tttcaaagtt ggtcagttcg
     4801 gttcccttat gattgaccgt ctgcgcctcg ttccggctaa gtaacatgga gcaggtcgcg
     4861 gatttcgaca caatttatca ggcgatgata caaatctccg ttgtactttg tttcgcgctt
     4921 ggtataatcg ctgggggtca aagatgagtg ttttagtgta ttctttcgcc tctttcgttt
     4981 taggttggtg ccttcgtagt ggcattacgt attttacccg tttaatggaa acttcctcat
     5041 gaaaaagtct ttagccctca aagcctctgt agccgttgct accctcgttc cgatgctgtc
     5101 tttcgctgct gagggtgacg atcccgcaaa agcggccttt aactccctgc aagcctcagc
     5161 gaccgaatat atcggttatg cgtgggcgat ggttgttgtc attgtcggcg caactatcgg
     5221 tatcaagctg tttaagaaat tcacctcgaa agcaagctga taaaccgata caattaaagg
     5281 ctccttttgg agcctttttt tttggagatt ttcaacgtga aaaaattatt attcgcaatt
     5341 cctttagttg ttcctttcta ttctcactcc gctgaaactg ttgaaagttg tttagcaaaa
     5401 ccccatacag aaaattcatt tactaacgtc tggaaagacg acaaaacttt agatcgttac
     5461 gctaactatg agggctgtct gtggaatgct acaggcgttg tagtttgtac tggtgacgaa
     5521 actcagtgtt acggtacatg ggttcctatt gggcttgcta tccctgaaaa tgagggtggt
     5581 ggctctgagg gtggcggttc tgagggtggc ggttctgagg gtggcggtac taaacctcct
     5641 gagtacggtg atacacctat tccgggctat acttatatca accctctcga cggcacttat
     5701 ccgcctggta ctgagcaaaa ccccgctaat cctaatcctt ctcttgagga gtctcagcct
     5761 cttaatactt tcatgtttca gaataatagg ttccgaaata ggcagggggc attaactgtt
     5821 tatacgggca ctgttactca aggcactgac cccgttaaaa cttattacca gtacactcct
     5881 gtatcatcaa aagccatgta tgacgcttac tggaacggta aattcagaga ctgcgctttc
     5941 cattctggct ttaatgagga tccattcgtt tgtgaatatc aaggccaatc gtctgacctg
     6001 cctcaacctc ctgtcaatgc tggcggcggc tctggtggtg gttctggtgg cggctctgag
     6061 ggtggtggct ctgagggtgg cggttctgag ggtggcggct ctgagggagg cggttccggt
     6121 ggtggctctg gttccggtga ttttgattat gaaaagatgg caaacgctaa taagggggct
     6181 atgaccgaaa atgccgatga aaacgcgcta cagtctgacg ctaaaggcaa acttgattct
     6241 gtcgctactg attacggtgc tgctatcgat ggtttcattg gtgacgtttc cggccttgct
     6301 aatggtaatg gtgctactgg tgattttgct ggctctaatt cccaaatggc tcaagtcggt
     6361 gacggtgata attcaccttt aatgaataat ttccgtcaat atttaccttc cctccctcaa
     6421 tcggttgaat gtcgcccttt tgtctttggc gctggtaaac catatgaatt ttctattgat
     6481 tgtgacaaaa taaacttatt ccgtggtgtc tttgcgtttc ttttatatgt tgccaccttt
     6541 atgtatgtat tttctacgtt tgctaacata ctgcgtaata aggagtctta atcatgccag
     6601 ttcttttggg tattccgtta ttattgcgtt tcctcggttt ccttctggta actttgttcg
     6661 gctatctgct tacttttctt aaaaagggct tcggtaagat agctattgct atttcattgt
     6721 ttcttgctct tattattggg cttaactcaa ttcttgtggg ttatctctct gatattagcg
     6781 ctcaattacc ctctgacttt gttcagggtg ttcagttaat tctcccgtct aatgcgcttc
     6841 cctgttttta tgttattctc tctgtaaagg ctgctatttt catttttgac gttaaacaaa
     6901 aaatcgtttc ttatttggat tgggataaat aatatggctg tttattttgt aactggcaaa
     6961 ttaggctctg gaaagacgct cgttagcgtt ggtaagattc aggataaaat tgtagctggg
     7021 tgcaaaatag caactaatct tgatttaagg cttcaaaacc tcccgcaagt cgggaggttc
     7081 gctaaaacgc ctcgcgttct tagaataccg gataagcctt ctatatctga tttgcttgct
     7141 attgggcgcg gtaatgattc ctacgatgaa aataaaaacg gcttgcttgt tctcgatgag
     7201 tgcggtactt ggtttaatac ccgttcttgg aatgataagg aaagacagcc gattattgat
     7261 tggtttctac atgctcgtaa attaggatgg gatattattt ttcttgttca ggacttatct
     7321 attgttgata aacaggcgcg ttctgcatta gctgaacatg ttgtttattg tcgtcgtctg
     7381 gacagaatta ctttaccttt tgtcggtact ttatattctc ttattactgg ctcgaaaatg
     7441 cctctgccta aattacatgt tggcgttgtt aaatatggcg attctcaatt aagccctact
     7501 gttgagcgtt ggctttatac tggtaagaat ttgtataacg catatgatac taaacaggct
     7561 ttttctagta attatgattc cggtgtttat tcttatttaa cgccttattt atcacacggt
     7621 cggtatttca aaccattaaa tttaggtcag aagatgaaat taactaaaat atatttgaaa
     7681 aagttttctc gcgttctttg tcttgcgatt ggatttgcat cagcatttac atatagttat
     7741 ataacccaac ctaagccgga ggttaaaaag gtagtctctc agacctatga ttttgataaa
     7801 ttcactattg actcttctca gcgtcttaat ctaagctatc gctatgtttt caaggattct
     7861 aagggaaaat taattaatag cgacgattta cagaagcaag gttattcact cacatatatt
     7921 gatttatgta ctgtttccat taaaaaaggt aattcaaatg aaattgttaa atgtaattaa
     7981 ttttgttttc ttgatgtttg tttcatcatc ttcttttgct caggtaattg aaatgaataa
     8041 ttcgcctctg cgcgattttg taacttggta ttcaaagcaa tcaggcgaat ccgttattgt
     8101 ttctcccgat gtaaaaggta ctgttactgt atattcatct gacgttaaac ctgaaaatct
     8161 acgcaatttc tttatttctg ttttacgtgc aaataatttt gatatggtag gttctaaccc
     8221 ttccattatt cagaagtata atccaaacaa tcaggattat attgatgaat tgccatcatc
     8281 tgataatcag gaatatgatg ataattccgc tccttctggt ggtttctttg ttccgcaaaa
     8341 tgataatgtt actcaaactt ttaaaattaa taacgttcgg gcaaaggatt taatacgagt
     8401 tgtcgaattg tttgtaaagt ctaatacttc taaatcctca aatgtattat ctattgacgg
     8461 ctctaatcta ttagttgtta gtgctcctaa agatatttta gataaccttc ctcaattcct
     8521 ttcaactgtt gatttgccaa ctgaccagat attgattgag ggtttgatat ttgaggttca
     8581 gcaaggtgat gctttagatt tttcatttgc tgctggctct cagcgtggca ctgttgcagg
     8641 cggtgttaat actgaccgcc tcacctctgt tttatcttct gctggtggtt cgttcggtat
     8701 ttttaatggc gatgttttag ggctatcagt tcgcgcatta aagactaata gccattcaaa
     8761 aatattgtct gtgccacgta ttcttacgct ttcaggtcag aagggttcta tctctgttgg
     8821 ccagaatgtc ccttttatta ctggtcgtgt gactggtgaa tctgccaatg taaataatcc
     8881 atttcagacg attgagcgtc aaaatgtagg tatttccatg agcgtttttc ctgttgcaat
     8941 ggctggcggt aatattgttc tggatattac cagcaaggcc gatagtttga gttcttctac
     9001 tcaggcaagt gatgttatta ctaatcaaag aagtattgct acaacggtta atttgcgtga
     9061 tggacagact cttttactcg gtggcctcac tgattataaa aacacttctc aggattctgg
     9121 cgtaccgttc ctgtctaaaa tccctttaat cggcctcctg tttagctccc gctctgattc
     9181 taacgaggaa agcacgttat acgtgctcgt caaagcaacc atagtacgcg ccctgtagca
     9241 tgcaggagtc actaagggtt agttagttag attagcagaa agtcaaaagc ctccgaccgg
     9301 aggcttttga ctaaaacttc ccttggggtt atcattgggg ctcactcaaa ggcggtaatc
     9361 agataaaaaa aatccttagc tttcgctaag gatgatttct gctagtatta ttagaaaaac
     9421 tcatcgagca tcaaatgaaa ctgcaatt
//
