LOCUS       sequence-250020-        2620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the 3' 500 bp hThal5.10-2-hg18 locus homology arm as a type 
            8b part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK8b_009
FEATURES             Location/Qualifiers
     source          1..2620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      77..665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     566..585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1751..1785)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1856..2515)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2430..2449
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg tctcacaatt
      781 agaaatgtat gaattaaatt ggattaatca caatatatac tatataaaaa agtatgcagt
      841 acataaatac acagatacaa aatataggca tcatttttga agttatgtta taatatattg
      901 aggctatttt attagaaaac gaactttatg ctaactgtat aaaggaaaaa agaataattg
      961 cagaagaaat aatttataat ttgcttttcc ttggttttat ttaaaaatta aattaattaa
     1021 attataagta tattgtttcc atataattta taataaataa ggcatatagg ccaggcacgg
     1081 tggctcatgc ctgtaatccc agcactttgg gaggcagagg tgggtgaacg cctgaggtca
     1141 ggcgttcaag accagcctgg ccaacatggt gaaacctcgt ctgtacaaaa aatacaaaaa
     1201 tcagctgggc atggtgtcag atgcctgtaa tcccaggtac ttgggaggct gaggcaggag
     1261 aatctcttga acccaggagg cagaggttgc agtgagccga gatcatgcca ttgcactcct
     1321 gcctggggga caagagcgag acttcgtcta aaaaacaaaa caaaacaaaa caaaaacaaa
     1381 aaaaaacaca aagaaacaaa aaaccattta aagcaacaaa ttactcattt cttagctata
     1441 ttctgtttaa ttaaaaaata gggtatcctc tatgtaaaga aaaatataat gttattgagc
     1501 cattttcttc agaagaacaa gctactctac ctatgtagag tgataatgta tcctcaggat
     1561 tgaagatcaa aagaggtaat atatacaaac attctttgaa aggtgcctta taaaacaaaa
     1621 aattgtttta tcataaaaat ttaaggtagt gcagagagct aacatgaaaa atgtaaacat
     1681 tggcaataag aacaaagata tactatgttt gaaaaatggt tgaagtccct ttccccttga
     1741 gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     1801 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
     1861 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     1921 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     1981 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     2041 ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa
     2101 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     2161 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     2221 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     2281 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     2341 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     2401 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     2461 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     2521 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     2581 cattatggtg aaagttggaa cctcttacgt gcccgatcaa
//
