LOCUS       sequence-250088-        3731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBC with 4 repeats of 80bp DNA template.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Chavan D, Chen H, Crum M, Vu B, Safari M, Smith M, Vekilov P, Conrad
            JC, Kourentzi K, Willson RC
  TITLE     Neutral DNA-avidin nanoparticles as ultrasensitive reporters in 
            immuno-PCR.
  JOURNAL   Analyst. 2020 Jul 21;145(14):4942-4949. doi: 10.1039/d0an00134a. 
            Epub 2020 Jun 5.
  PUBMED    32500871
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/d0an00134a"; journalName: "Analyst"; date: "2020-07-21- 
            21"; volume: "145"; issue: "14"; pages: "4942-4949"
COMMENT     Sequence Label: pMCRW2
FEATURES             Location/Qualifiers
     source          1..3731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1398..1414
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1398..1414
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(1448..1464)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1449..1465)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(1489..1508)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1490..1508)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1518..1535)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1518..1534)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1527..1549)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1676..2131
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1763..1782)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1973..1994
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2157..2261
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(2750..3409)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3324..3343
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3410..3512)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagcttca ggtagtagta cgtctgtttc acgatgagac
     1081 tggatgaatc acggtagcat aaggtgcaag ataatactct cgcagcagag cttcaggtag
     1141 tagtacgtct gtttcacgat gagactggat gaatcacggt agcataaggt gcaagataat
     1201 actctcgcag cagagcttca ggtagtagta cgtctgtttc acgatgagac tggatgaatc
     1261 acggtagcat aaggtgcaag ataatactct cgcagcagag cttcaggtag tagtacgtct
     1321 gtttcacgat gagactggat gaatcacggt agcataaggt gcaagataat actctcgcag
     1381 cagagctcat gtcaccatct agaactagtg gatcccccgg gctgcaggaa ttcgatatca
     1441 agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag
     1501 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     1561 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     1621 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     1681 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     1741 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     1801 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     1861 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     1921 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     1981 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     2041 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     2101 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     2161 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     2221 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     2281 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     2341 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     2401 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     2461 tgagcacttt ttgatcagaa aaaaaggatc atatcgtcaa ttattacctc cacggggaga
     2521 gcctgagcaa actggcctca ggcatttgag aagcacacgg tcacactgct tccggtagtc
     2581 aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg
     2641 acgaccgggt cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc
     2701 aggcgtagca accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc
     2761 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     2821 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     2881 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     2941 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
     3001 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     3061 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     3121 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     3181 ttgccatacg taattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     3241 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     3301 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     3361 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     3421 tagctcctga aaatctcgac aactcaaaaa atacgcccgg tagtgatctt atttcattat
     3481 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc
     3541 agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg
     3601 tcacaggtat ttattcggtc gaaaaggatc taggtgaaga tcctttttga taatctcatg
     3661 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3721 aaaggatctt c
//
