LOCUS       sequence-250334-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FAST (also called YFAST) fused to zebrafish histone H2B in
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Plamont MA, Billon-Denis E, Maurin S, Gauron C, Pimenta FM, Specht 
            CG, Shi J, Querard J, Pan B, Rossignol J, Moncoq K, Morellet N, 
            Volovitch M, Lescop E, Chen Y, Triller A, Vriz S, Le Saux T, Jullien
            L, Gautier A
  TITLE     Small fluorescence-activating and absorption-shifting tag for 
            tunable protein imaging in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):497-502. doi: 
            10.1073/pnas.1513094113. Epub 2015 Dec 28.
  PUBMED    26711992
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1513094113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-01-19- 19"; volume: "113"; issue: "3"; pages: "497-502"
COMMENT     Sequence Label: pAG109 H2B-FAST
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      403..412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             409..783
                     /codon_start=1
                     /product="small protein tag, derived from photoactive 
                     yellow protein, that allows in vivo fluorescent labeling by
                     addition of a cell-permeant fluorogen (Plamont et al., 
                     2016)"
                     /label=Y-FAST
                     /translation="MEHVAFGSEDIENTLAKMDDGQLDGLAFGAIQLDGDGNILQYNAA
                     EGDITGRDPKQVIGKNFFKDVAPGTDSPEFYGKFKEGVASGNLNTMFEWMIPTSRGPTK
                     VKVHMKKALSGDSYWVFVKRV"
ORIGIN
        1 atgcccgaac ctgcgaagtc agcgcccgct cccaaaaaag gctctaaaaa agctgtcgcc
       61 aagacccaga agaaggggga taagaaaagg cgtaagacca ggaaagagag ttacgccatt
      121 tacgtgtaca aagtactaaa acaagtccac ccggacactg gcatctcctc aaaggcgatg
      181 ggcattatga actcatttgt aaacgacatc ttcgagcgca tcgccggaga agcgtcgcgc
      241 ctggcgcatt acaacaagcg ctccactatc acatcccggg agatccagac ggccgtgcgc
      301 ctgctcctgc ccggagaact ggccaaacac gctgtgtctg agggcacaaa ggccgtgacc
      361 aagtacacca gctccaaggg cggaggctcc ggaggcggat ctgccaccat ggagcatgtt
      421 gcctttggca gtgaggacat cgagaacact ctggccaaaa tggacgacgg acaactggat
      481 gggttggcct ttggcgcaat tcagctcgat ggtgacggga atatcctgca gtacaatgct
      541 gctgaaggag acatcacagg cagagatccc aaacaggtga ttgggaagaa cttcttcaag
      601 gatgttgcac ctggaacgga ttctcccgag ttttacggca aattcaagga aggcgtagcg
      661 tcagggaatc tgaacaccat gttcgaatgg atgataccga caagcagggg accaaccaag
      721 gtcaaggtgc acatgaagaa agccctttcc ggtgacagct attgggtctt tgtgaaacgg
      781 gtg
//
