LOCUS       sequence-250339-         375 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses His-tagged iFAST in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Tebo AG, Pimenta FM, Zhang Y, Gautier A
  TITLE     Improved Chemical-Genetic Fluorescent Markers for Live Cell 
            Microscopy.
  JOURNAL   Biochemistry. 2018 Oct 2;57(39):5648-5653. doi: 
            10.1021/acs.biochem.8b00649. Epub 2018 Sep 17.
  PUBMED    30204425
REFERENCE   2  (bases 1 to 375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.8b00649"; journalName: "Biochemistry"; date: 
            "2018-10-2- 2"; volume: "57"; issue: "39"; pages: "5648-5653"
COMMENT     Sequence Label: pAG247 His-iFAST
FEATURES             Location/Qualifiers
     source          1..375
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..375
                     /codon_start=1
                     /product="small protein tag, derived from photoactive 
                     yellow protein, that allows in vivo fluorescent labeling by
                     addition of a cell-permeant fluorogen (Plamont et al., 
                     2016)"
                     /label=Y-FAST
                     /translation="MEHVAFGSEDIENTLAKMDDGQLDGLAFGAIQLDGDGNILQYNAA
                     EGDITGRDPKQVIGKNFFKDVAPGTDSPEFYGKFKEGVASGNLNTMFEWMIPTSRGPTK
                     VKIHMKKALSGDSYWVFVKRV"
ORIGIN
        1 atggagcatg ttgcctttgg cagtgaggac atcgagaaca ctctggccaa aatggacgac
       61 ggacaactgg atgggttggc ctttggcgca attcagctcg atggtgacgg gaatatcctg
      121 cagtacaatg ctgctgaagg agacatcaca ggcagagatc ccaaacaggt gattgggaag
      181 aacttcttca aggatgttgc acctggaacg gattctcccg agttttacgg caaattcaag
      241 gaaggcgtag cgtcagggaa tctgaacacc atgttcgaat ggatgatacc gacaagcagg
      301 ggaccaacca aggtcaagat acacatgaag aaagcccttt ccggtgacag ctattgggtc
      361 tttgtgaaac gggtg
//
