LOCUS       sequence-25038-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Venken KJ, Kasprowicz J, Kuenen S, Yan J, Hassan BA, Verstreken P
  TITLE     Recombineering-mediated tagging of Drosophila genomic constructs for
            in vivo localization and acute protein inactivation.
  JOURNAL   Nucleic Acids Res. 2008 Oct;36(18):e114. doi: 10.1093/nar/gkn486. 
            Epub 2008 Aug 1.
  PUBMED    18676454
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2008-10"; volume: "36(18):e114. doi"; pages: " 
            10.1093/nar/gkn486. Epub 2008 Aug 1"
COMMENT     Sequence Label: PL-452 C-EGFP
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(121..138)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    127..351
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    408..441
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(507..528)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gggctgancg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
       61 tctatcgcct tcttgacgag ttcttctgag gggatcaatt ctctagagct cgctgatcag
      121 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
      181 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
      241 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
      301 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
      361 cggaaagaac cagctggggc tcgactagag cttgcggaac ccttaatata acttcgtata
      421 atgtatgcta tacgaagtta ttaggtccct cgaggggatc catggtgagc aagggcgagg
      481 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      541 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      601 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      661 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      721 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcanngac gacggcanct
      781 acaagacccg cgccnnngtg aagttcgagg gcga
//
