LOCUS       sequence-25040-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Venken KJ, Kasprowicz J, Kuenen S, Yan J, Hassan BA, Verstreken P
  TITLE     Recombineering-mediated tagging of Drosophila genomic constructs for
            in vivo localization and acute protein inactivation.
  JOURNAL   Nucleic Acids Res. 2008 Oct;36(18):e114. doi: 10.1093/nar/gkn486. 
            Epub 2008 Aug 1.
  PUBMED    18676454
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2008-10"; volume: "36(18):e114. doi"; pages: " 
            10.1093/nar/gkn486. Epub 2008 Aug 1"
COMMENT     Sequence Label: PL-452 C-Flag-4C
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(121..138)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    127..351
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    408..441
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             465..488
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             513..530
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     primer_bind     complement(564..583)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(565..583)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(590..607)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(590..606)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(599..621)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
       61 tctatcgcct tcttgacgag ttcttctgag gggatcaatt ctctagagct cgctgatcag
      121 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
      181 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
      241 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
      301 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
      361 cggaaagaac cagctggggc tcgactagag cttgcggaac ccttaatata acttcgtata
      421 atgtatgcta tacgaagtta ttaggtccct cgaggggatc catggattac aaggatgacg
      481 acgataaggg ctccgccgga tcgtttttga attgctgccc cggctgctgc atggagccct
      541 aaccgcggtg gagctccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg
      601 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      661 atcccccttt cgccagctgg cgtaatagcg aagaggcnnn cacnnatcgc ccttcccaac
      721 agttgcgcag cctg
//
