LOCUS       sequence-250469-        1131 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dominant negative mutant mouse FGFR1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Bordignon P, Bottoni G, Xu X, Popescu AS, Truan Z, Guenova E, Kofler
            L, Jafari P, Ostano P, Rocken M, Neel V, Dotto GP
  TITLE     Dualism of FGF and TGF-beta Signaling in Heterogeneous 
            Cancer-Associated Fibroblast Activation with ETV1 as a Critical 
            Determinant.
  JOURNAL   Cell Rep. 2019 Aug 27;28(9):2358-2372.e6. doi: 
            10.1016/j.celrep.2019.07.092.
  PUBMED    31461652
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.07"; journalName: "Cell Rep"; date: 
            "2019-08-27- 27"; volume: "28"; issue: "9"; pages: "2358-2372.e6"
COMMENT     Sequence Label: pLenti-CMV-Blast-DNFGFR1-HA
FEATURES             Location/Qualifiers
     source          1..1131
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..27
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    111..135
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
       61 catagaagac accgactcta gtccagtgtg gtggaattct gcagatatca acaagtttgt
      121 acaaaaaagc aggctccgcg gccgccccct tcaccatgtg gggctggaag tgcctcctct
      181 tctgggctgt gctggtcaca gccactctct gcactgccag gccagcccca accttgcccg
      241 aacaagatgc actcccatcc tcggaagatg atgacgacga cgatgactcc tcctcggagg
      301 agaaagagac ggacaacacc aaaccaaacc gtaggcctgt agctccctac tggacatccc
      361 cagagaaaat ggagaagaaa ctgcatgcgg tgcccgctgc caagacggtg aagttcaagt
      421 gcccgtcgag tgggacaccc aaccccactc tgcgctggtt gaaaaatggc aaagagttta
      481 agcctgacca ccgaattgga ggctacaagg ttcgctatgc cacctggagc atcataatgg
      541 attctgtggt gccttctgac aagggcaact acacctgcat cgtggagaat gagtatggga
      601 gcatcaacca cacctaccag cttgacgtcg tggaacgatc tccgcaccga cccatccttc
      661 aggcagggct gcctgccaac aagacagtgg ccctgggcag caatgtggag ttcatgtgta
      721 aggtgtacag cgatccgcag cctcacattc agtggctgaa gcacatcgag gtgaacggga
      781 gtaagatcgg gccagacaac ttgccgtatg tccagatcct gaagactgct ggagttaata
      841 ccaccgacaa ggaaatggag gtgcttcatc tacggaatgt ctcctttgag gatgcggggg
      901 agtatacgtg cttggcgggt aactctatcg gactctccca tcactctgca tggttgaccg
      961 ttctggaagc cctggaagag agaccagctg tgatgacctc accgctctac ctggagatca
     1021 ttatctactg caccggggcc ttcctgatct cctgcatgtt gggctctgtc atcatctata
     1081 agatgaagag cggcaccaag aagagcgact tccatagcca gatggctgtg c
//
