LOCUS       sequence-250472-       12145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR A289T.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12145)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-A289T
FEATURES             Location/Qualifiers
     source          1..12145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    267..384
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        432..1610
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          663..1601
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1558..1578
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1630..1654
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1657..1728,1729..3591,3592..3660,3661..5289)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGTTCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5291..5315)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5368..5409
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5430..5997
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5447..5999
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5591..5608)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5818..5837
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6011..6013,6014..6016,6017..6730)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6011..6013,6014..6016,6017..6727)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6056..6077)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6317..6336)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6664..6685
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6744..7332
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6797..6817)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7215..7226)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7407..7640
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7759..7777)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7845..7949
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7950..8018,8019..8810)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8981..9569
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9470..9489
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9723..9740
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9980..10001
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10016..10021,10022..10039,10040..10046)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10054..10070
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10059..10081
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10078..10094
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10078..10094
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10144..10163
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10355..10988
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11035..11160
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11657..11890
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12075..12119
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
       61 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
      121 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
      181 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat
      241 tcacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
      301 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
      361 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
      421 actagtcgtg aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
      481 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
      541 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
      601 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
      661 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
      721 tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg
      781 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
      841 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
      901 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
      961 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
     1021 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
     1081 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg
     1141 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
     1201 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
     1261 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
     1321 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca
     1381 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
     1441 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
     1501 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
     1561 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga agcggccctg
     1621 cagatatcaa caagtttgta caaaaaagca ggcaccatgc gaccctccgg gacggccggg
     1681 gcagcgctcc tggcgctgct ggctgcgctc tgcccggcga gtcgggctct ggaggaaaag
     1741 aaagtttgcc aaggcacgag taacaagctc acgcagttgg gcacttttga agatcatttt
     1801 ctcagcctcc agaggatgtt caataactgt gaggtggtcc ttgggaattt ggaaattacc
     1861 tatgtgcaga ggaattatga tctttccttc ttaaagacca tccaggaggt ggctggttat
     1921 gtcctcattg ccctcaacac agtggagcga attcctttgg aaaacctgca gatcatcaga
     1981 ggaaatatgt actacgaaaa ttcctatgcc ttagcagtct tatctaacta tgatgcaaat
     2041 aaaaccggac tgaaggagct gcccatgaga aatttacagg aaatcctgca tggcgccgtg
     2101 cggttcagca acaaccctgc cctgtgcaac gtggagagca tccagtggcg ggacatagtc
     2161 agcagtgact ttctcagcaa catgtcgatg gacttccaga accacctggg cagctgccaa
     2221 aagtgtgatc caagctgtcc caatgggagc tgctggggtg caggagagga gaactgccag
     2281 aaactgacca aaatcatctg tgcccagcag tgctccgggc gctgccgtgg caagtccccc
     2341 agtgactgct gccacaacca gtgtgctgca ggctgcacag gcccccggga gagcgactgc
     2401 ctggtctgcc gcaaattccg agacgaagcc acgtgcaagg acacctgccc cccactcatg
     2461 ctctacaacc ccaccacgta ccagatggat gtgaaccccg agggcaaata cagctttggt
     2521 accacctgcg tgaagaagtg tccccgtaat tatgtggtga cagatcacgg ctcgtgcgtc
     2581 cgagcctgtg gggccgacag ctatgagatg gaggaagacg gcgtccgcaa gtgtaagaag
     2641 tgcgaagggc cttgccgcaa agtgtgtaac ggaataggta ttggtgaatt taaagactca
     2701 ctctccataa atgctacgaa tattaaacac ttcaaaaact gcacctccat cagtggcgat
     2761 ctccacatcc tgccggtggc atttaggggt gactccttca cacatactcc tcctctggat
     2821 ccacaggaac tggatattct gaaaaccgta aaggaaatca cagggttttt gctgattcag
     2881 gcttggcctg aaaacaggac ggacctccat gcctttgaga acctagaaat catacgcggc
     2941 aggaccaagc aacatggtca gttttctctt gcagtcgtca gcctgaacat aacatccttg
     3001 ggattacgct ccctcaagga gataagtgat ggagatgtga taatttcagg aaacaaaaat
     3061 ttgtgctatg caaatacaat aaactggaaa aaactgtttg ggacctccgg tcagaaaacc
     3121 aaaattataa gcaacagagg tgaaaacagc tgcaaggcca caggccaggt ctgccatgcc
     3181 ttgtgctccc ccgagggctg ctggggcccg gagcccaggg actgcgtctc ttgccggaat
     3241 gtcagccgag gcagggaatg cgtggacaag tgcaaccttc tggagggtga gccaagggag
     3301 tttgtggaga actctgagtg catacagtgc cacccagagt gcctgcctca ggccatgaac
     3361 atcacctgca caggacgggg accagacaac tgtatccagt gtgcccacta cattgacggc
     3421 ccccactgcg tcaagacctg cccggcagga gtcatgggag aaaacaacac cctggtctgg
     3481 aagtacgcag acgccggcca tgtgtgccac ctgtgccatc caaactgcac ctacggatgc
     3541 actgggccag gtcttgaagg ctgtccaacg aatgggccta agatcccgtc catcgccact
     3601 gggatggtgg gggccctcct cttgctgctg gtggtggccc tggggatcgg cctcttcatg
     3661 cgaaggcgcc acatcgttcg gaagcgcacg ctgcggaggc tgctgcagga gagggagctt
     3721 gtggagcctc ttacacccag tggagaagct cccaaccaag ctctcttgag gatcttgaag
     3781 gaaactgaat tcaaaaagat caaagtgctg ggctccggtg cgttcggcac ggtgtataag
     3841 ggactctgga tcccagaagg tgagaaagtt aaaattcccg tcgctatcaa ggaattaaga
     3901 gaagcaacat ctccgaaagc caacaaggaa atcctcgatg aagcctacgt gatggccagc
     3961 gtggacaacc cccacgtgtg ccgcctgctg ggcatctgcc tcacctccac cgtgcagctc
     4021 atcacgcagc tcatgccctt cggctgcctc ctggactatg tccgggaaca caaagacaat
     4081 attggctccc agtacctgct caactggtgt gtgcagatcg caaagggcat gaactacttg
     4141 gaggaccgtc gcttggtgca ccgcgacctg gcagccagga acgtactggt gaaaacaccg
     4201 cagcatgtca agatcacaga ttttgggctg gccaaactgc tgggtgcgga agagaaagaa
     4261 taccatgcag aaggaggcaa agtgcctatc aagtggatgg cattggaatc aattttacac
     4321 agaatctata cccaccagag tgatgtctgg agctacgggg tgaccgtttg ggagttgatg
     4381 acctttggat ccaagccata tgacggaatc cctgccagcg agatctcctc catcctggag
     4441 aaaggagaac gcctccctca gccacccata tgtaccatcg atgtctacat gatcatggtc
     4501 aagtgctgga tgatagacgc agatagtcgc ccaaagttcc gtgagttgat catcgaattc
     4561 tccaaaatgg cccgagaccc ccagcgctac cttgtcattc agggggatga aagaatgcat
     4621 ttgccaagtc ctacagactc caacttctac cgtgccctga tggatgaaga agacatggac
     4681 gacgtggtgg atgccgacga gtacctcatc ccacagcagg gcttcttcag cagcccctcc
     4741 acgtcacgga ctcccctcct gagctctctg agtgcaacca gcaacaattc caccgtggct
     4801 tgcattgata gaaatgggct gcaaagctgt cccatcaagg aagacagctt cttgcagcga
     4861 tacagctcag accccacagg cgccttgact gaggacagca tagacgacac cttcctccca
     4921 gtgcctgaat acataaacca gtccgttccc aaaaggcccg ctggctctgt gcagaatcct
     4981 gtctatcaca atcagcctct gaaccccgcg cccagcagag acccacacta ccaggacccc
     5041 cacagcactg cagtgggcaa ccccgagtat ctcaacactg tccagcccac ctgtgtcaac
     5101 agcacattcg acagccctgc ccactgggcc cagaaaggca gccaccaaat tagcctggac
     5161 aaccctgact accagcagga cttctttccc aaggaagcca agccaaatgg catctttaag
     5221 ggctccacag ctgaaaatgc agaataccta agggtcgcgc cacaaagcag tgaatttatt
     5281 ggagcataga acccagcttt cttgtacaaa gtggttgata tccagcacag tggcggccgc
     5341 tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc
     5401 gattctacgc gtaccggtta gtaatgagat ccctcccccc cccctaacgt tactggccga
     5461 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     5521 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     5581 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     5641 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     5701 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     5761 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     5821 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     5881 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     5941 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat
     6001 ggccacacat atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     6061 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     6121 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     6181 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     6241 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     6301 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     6361 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     6421 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     6481 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     6541 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     6601 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     6661 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     6721 gtacaagtaa atcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga
     6781 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6841 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6901 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     6961 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     7021 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7081 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     7141 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7201 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7261 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7321 ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg tagatcttag
     7381 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     7441 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7501 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7561 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7621 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7681 tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa gggctagatc
     7741 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     7801 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7861 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7921 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7981 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     8041 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     8101 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     8161 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     8221 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     8281 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     8341 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     8401 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8461 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     8521 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8581 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8641 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8701 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8761 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8821 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8881 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8941 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     9001 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     9061 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     9121 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     9181 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9241 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9301 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9361 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9421 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9481 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9541 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9601 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9661 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     9721 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     9781 cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc
     9841 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     9901 aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg
     9961 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    10021 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    10081 gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca ctgcattcta
    10141 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
    10201 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
    10261 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
    10321 gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga
    10381 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
    10441 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
    10501 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
    10561 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
    10621 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
    10681 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
    10741 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
    10801 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
    10861 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
    10921 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
    10981 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
    11041 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
    11101 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
    11161 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
    11221 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
    11281 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
    11341 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
    11401 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
    11461 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
    11521 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
    11581 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
    11641 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
    11701 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
    11761 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
    11821 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
    11881 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
    11941 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
    12001 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
    12061 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
    12121 ggaattggtt taacataaca aattg
//
